Abstract
The relative sensitivity of tannery effluent before and after treatment was compared by employing Nostoc muscorum and microtox assay in laboratory. The effect on chlorophyll, protein and biomass content of Nostoc muscorum was studied with the luminescent property inhibition of Photobacterium phosphorium and compared with algal bioassay. The results of microtox assay after 5, 15 and 30 min of exposure were compared with data obtained from algal bioassay. It was observed that the luminescent property of Photobacterium phosphorium in microtox assay as well as the chlorophyll content of Nostoc muscorum in algal assay were the most sensitive parameters in toxicity evaluation of tannery effluent. The microtox assay produced notably comparable EC50 values with that of algalbioassay. The microtox assay of toxicity showed that EC50 (%) in 30 min was 3.19 and 63.49 for untreated and treated tannery effluent, respectively while in algal bioassay the EC50 for chlorophyll was in between 0–2.5% and 100%, respectively, in untreated and treated effluent. More than 60% reduction of toxicity was noted in treated tannery effluent in both test system.
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Alexander, M.: 1995, ‘How toxic are chemicals in soil’, Environ. Sci. Technol. 29, 2713–2713.
APHA: 1992, Standard Methods for the Examination of Water and Wastewater, 18th ed., American Public Health Association, Washington, D.C.
Arnon, D. I.: 1949, ‘Copper enzymes in isolated chloroplasti polyphenol oxidase in Beta vulgaris’, Plant Physiol. 24, 1–15.
Athey, L. A., Thomas, J. M., Miller, W. E. and Word, J. Q.: 1989, ‘Evaluation of bioassys for designing sediment cleanup strategies at a wood treatment site’, Environ. Toxicol. Chem. 7, 223–230.
Backhaus, T. and Grimme, L. H.: 1999, ‘The toxicity of antibiotics agents to the luminescent bacterium Vibrio fischeri’, Chemosphere 38(14), 3291–3301.
Baun, A., Kasper, B., Justesen, Niels Nyholm: 2002, ‘Algal tests with soil suspensions and eutriates: A comparative evaluation for PAH contaminated soils’, Chemosphere 46, 251–258.
Blaise, C. R.: 1986, ‘Micromethod for acute aquatic toxicity assessment using the green alga Selenastrum capricornutum’, Toxicity Assessment: Inter. Quart. 1, 377–385.
Boluda, R., Quintanills, J. E., Bonilla, T. A., Saez, E. and Gamon, M.: 2002, ‘Application of the Microtox® test and pollution indices to the study of water toxicity in the Albufera Natural Park (Valencia, Spain)’, Chemosphere 46, 355–369.
Brierley, C. L. and Brierley, J. A.: 1993, in A. E. Torma, M. L. Apel and C. L. Brierley (eds), Biohydrometallurgical Technologies, 2. The Minerals, Metals and Materials Society, Warrendale, PA, pp. 35–44.
Bringmann, G. and Kuhn, R.: 1978a, ‘Testing of substances for their toxicity threshold: Model organisms Microcystis (Diplocystis) aeruginosa and Scenedesmus quadricauda’, Mitt. Int. Ver. Theor. Angew. Limnol. 21, 275–284.
Bundy, J. G., Wardell, J. L., Campbell, C. D., Killham, K. and Paton, G. I.: 1997, ‘Application of bioluminescence-based microbial biosensors to the ecotoxicity assessment of organotins’, Lett. Appl. Microbiol. 25, 353–358.
Coleman, Richard, N., Ansar, A. and Qureshi: 1985, ‘Microtox and Spirillum volutang tests for assessing toxicity of environmental samples’, Bull. Environ. Contam. Toxicol. 35, 443–451.
Dutka B. J. and Kwan K. K.: 1981, ‘Comparison of three microbial toxicity screening tests with the Microtox test’, Bull. Environm. Contam. Toxicol. 27, 753–757.
EPA: 1993, Methods for Aquatic Toxicity Identification Evaluations: Phase II Toxicity Identification Procedures for Samples Exhibiting Acute and Chromic Toxicity.
Guzzella, L.: 1998, ‘Comparition of tests procedures for sediments toxicity evaluation with Vibrio fischeri bacteria’, Chemosphere 37(14–15), 2895–2909.
Harkey, G. A., Landrum, P. F. and Klaine, S. J.: 1994, ‘Comparison of whole-sediment, elutriate and pore-water exposures for use in assessing sediment associated organic contaminants in bioassays’, Environ. Toxicol. Chem. 13(8), 1315–1329.
Joubert, G.: 1980, ‘A bioassay application for quantitative toxicity measurements using the green alga Selenastrum capricornutum’, Water Res. 14, 1759–1763.
Kaiser, K. L. E., Lum, K. R. and Palabrica, V. S.: 1988, ‘Review of field applications of the microtox test in great lakes and Saint Lawrence river water’, Water Pollut. Res. J. Canada 23(2), 270–278.
Khasim, I. D. and Nand Kumar, N. V.: 1989, ‘Environmental contamination of chromium in agricultural and animal products near a chromate industry’, Bull. Environ. Contam. Toxicol. 43, 742–746.
Kotrba, P., Doleckova, L., De Lorenzo, V. and Ruml, T.: 1999, ‘Enhanced bioaccumulation of heavy metals ions by bacterial cells due to surface display of short metal binding peptides’, Appl. Environ. Microbiol. 65(1), 1098–1098.
Lowry, O. H., Rosenbrough, N. J., Farr, P. A. L. and Randall, R. J.: 1951, ‘Protein measurement with folin phenol reagent’, J. Biol. Chem. 193, 265–275.
Miller, W. E., Peterson, S. A., Greene, J. C. and Callahan, C. A.: 1985, ‘Comparative toxicology of laboratory organisms for assessing hazardous waste site’, J. Environ. Qual. 14, 569–574.
OECD (Organisation for Economic Co-operation and Development): 1984, OECD Guidelines for the testing of chemicals I. Head of Publications Service OECD 2 rue Andrepascal 75775 PARIS CEDEX 16 France.
Okino, S., Iwasaki, K., Yagi, O. and Tanaka, H.: 2000, ‘Development of a biological mercury removal recovery system’, Biotechnol. Lett. 22, 783–788.
Prakasham, R. S., Sheno Merrie, J., Sheela, R., Sawathi, N. and Rama Krishna, S. V.: 1999, ‘Biosorption of chromium VI by free and immobilized Rhizopus arrhizus’, Environ. Pollut. 104, 421–427.
Qureshi, A. A., Coleman, R. N. and Paran, J. H.: 1984, ‘Evaluation and Refinement of the Microtox Test for Use in Toxicity Screening’, in D. Liu and B. J. Dutka (eds), Toxicity Screening Procedures using Bacterial System, Marcel Dekker, New York, pp. 1–22.
Rai, U. N. and Chandra, P.: 1989, ‘Removal of heavy metals from polluted water by Hydrodictyon reticulatum (Linn.) Lagerheim’, Sci. Total Environ. 87(88), 509–515.
Ribo, J. M.: 1987, ‘Interlaboratory comparison studies of the luminescent bacteria toxicity bioassay’, Environ. Toxicol. Water Qual. 12(4), 283–294.
Ruiz, M. J., Lopez-Jaramillo, L., Redondo, M. J. and Font, G.: 1997, ‘Toxicity assessment of pesticides using the microtox test: Application to environmental samples’, Bull. Environ. Contam. Toxicol. 59, 619–625.
Safferman, R. S. and Morris, M. E.: 1964, ‘Growth characteristics of the blue green algal virus LPP-1’, J. Bacteriol. 88, 771–775.
Thomas, J. M., Skalshi, J. R., Cline, J. F., McShane, M. C., Simpson, J. C., Miller, W. E., Peterson, S. A., Callahan, C. A. and Greene, J. C.: 1986, ‘Characterization of chemical waste site contamination and determination of its extent using bioassays’, Environ. Toxicol. Chem. 5, 487–501.
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Chandra, R., Pandey, P.K. & Srivastava, A. Comparative Toxicological Evaluation of Untreated and Treated Tannery Effluent with Nostoc Muscorum L. (Algal Assay) and Microtox Bioassay. Environ Monit Assess 95, 287–294 (2004). https://doi.org/10.1023/B:EMAS.0000029909.87977.a5
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DOI: https://doi.org/10.1023/B:EMAS.0000029909.87977.a5