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High-Fat Corn Oil Diet Promotes the Development of High Histologic Grade Rat DMBA-Induced Mammary Adenocarcinomas, While High Olive Oil Diet Does Not

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Abstract

Effects of a high corn oil and a high olive oil diet on the histopathologic characteristics of rat dimethylbenz(α)anthracene-induced mammary adenocarcinomas were investigated in comparison with those of a control low-fat diet. Two experimental series (A and B) studied the influence of a high corn oil diet on the initiation and the promotion of mammary carcinogenesis, while another one (C) assessed the effects of the two dietary lipids on the promotion. Nine parameters have been analyzed and a new histologic grading method, adapted to rat tumors, has been applied in each carcinoma. High corn oil diets, particularly when acting as promoters, associated with higher-grade carcinomas than control (p < 0.05) and high olive oil groups. Stromal invasion and tumoral necrosis were more prominent and a prevailing cribriform pattern was observed (p < 0.05). High olive oil diet adenocarcinomas exhibited a predominantly low histologic grade and few necrotic and invasive areas, similar to the control, and they presented the highest percentage of papillary areas. Lymphoplasmacytic and mast cell infiltration were also influenced by the dietary lipids. Thus, high corn oil diet adenocarcinomas presented a higher degree of morphological malignancy than control and high olive oil tumors, which is in line with the greater clinical malignancy described in rats from the former group and the non-promoting effect of the high olive oil diet. As far as we are concerned, a similar histopathologic approach of the effects of the dietary lipids on experimental breast cancer has not been carried out up to now.

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References

  1. Jemal A, Thomas A, Murray T, Thun M: Cancer statistics 2002. CA Cancer J Clin 52: 23–47, 2002

    PubMed  Google Scholar 

  2. World Cancer Research Foundation and American Institute for Cancer Research. Food, Nutrition and the Prevention of Cancer: a Global Perspective. World Cancer Research Foundation/American Institute for Cancer Research, Washington, DC, 1997.

  3. Clinton SK: Diet, anthropometry and breast cancer: integration of experimental and epidemiologic approaches. J Nutr 127: 916S–920S, 1997

    PubMed  Google Scholar 

  4. Bartsch H, Nair J, Owen RW: Dietary polyunsaturated fatty acids and cancers of the breast and colorectum: emerging evidence for their role as risk modifiers. Carcinogenesis 26: 2209–2218, 1999

    Article  Google Scholar 

  5. Welsch CW: Relationship between dietary fat and experimental mammary tumorigenesis: a review and critique. Cancer Res 52: 2040s–2048s, 1992

    PubMed  Google Scholar 

  6. Fay MP, Freedman LS, Clifford C, Midthune N: Effect of different types and amounts of fat on the development of mammary tumors in rodents: a review. Cancer Res 57: 3979–3988, 1997

    PubMed  Google Scholar 

  7. Ip C: Review of the effects of trans fatty acids, oleic acid, n–3 polyunsaturated fatty acids, and conjugated linoleic acid on mammary carcinogenesis in animals. Am J Clin Nutr 66: 1523S–1529S, 1997

    PubMed  Google Scholar 

  8. Lipworth L, Martí nez ME, Angell J, Hsieh C, Trichopoulos D: Olive oil and human cancer: an assessment of the evidence. Prev Med 26: 181–190, 1997

    Article  PubMed  Google Scholar 

  9. Zusman I, Gurevich P, Madar Z, Nyska A, Korol D, Timar B, Zuckerman A: Tumor–promoting and tumor–protective effects of high–fat diets on chemically induced mammary cancer in rats. Anticancer Res 17: 349–356, 1997

    PubMed  Google Scholar 

  10. Lee MM, Lin SS: Dietary fat and breast cancer. Annu Rev Nutr 20: 221–248, 2000

    Article  PubMed  Google Scholar 

  11. Escrich E: Validity of the DMBA–induced mammary cancer model for the study of human breast cancer. Int J Biol Markers 2: 197–206, 1987

    PubMed  Google Scholar 

  12. Costa I, Solanas M, Escrich E: Histopathologic characterization of mammary neoplastic lesions induced with 7,12 dimethylbenz(a)anthracene in the rat. A comparative analysis with human breast tumors. Arch Pathol Lab Med 126: 915–927, 2002

    PubMed  Google Scholar 

  13. Escrich E, Solanas M, Soler M, Ruiz de Villa MC, Sá nchez JA, Segura R: Dietary polyunsaturated n–6 lipids effects on the growth and fatty acid composition of rat mammary tumors. J Nutr Biochem 12: 536–549, 2001

    Article  PubMed  Google Scholar 

  14. Solanas M, Moral R, Escrich E: The stimulating effect of a high–fat n–6 polyunsaturated diet on rat DMBA–induced mammary tumors is not related to changes in c–Ha–ras1 mRNA tumor expression. Nutr Res 21: 1261–1273, 2001

    Article  Google Scholar 

  15. Solanas M, Hurtado A, Costa I, Moral R, Menendez JA, Colomer R, Escrich E: Effects of a high olive oil diet on the clinical behavior and histopathological features of rat DMBA–induced mammary tumors compared with a high corn oil diet. Int J Oncol 21: 745–753, 2002

    PubMed  Google Scholar 

  16. Moral R, Solanas M, García G, Colomer R, Escrich E: Modulation of EGFR and new expression by n–6 and n–9 high–fat diets in experimental mammary adenocarcinomas. Oncol Rep 10: 1417–1424, 2003

    PubMed  Google Scholar 

  17. Hopkins GH, Hard GC, West CE: Carcinogenesis induced by 7,12–dimethylbenza[a]anthracene in C3H–AvyfB mice: influence of different dietary fats. J Natl Cancer Inst 60: 849–853, 1978

    PubMed  Google Scholar 

  18. Fischer SM, Conti CJ, Locniskar M, Belury MA, Maldve RE, Lee ML, Leyton J, Slaga TJ, Bechtel DH: The effect of dietary fat on the rapid development of mammary tumors induced by 7,12–dimethylbenz(a)anthracene in SENCAR mice. Cancer Res 52: 662–666, 1992 I Costa et al. 234

    PubMed  Google Scholar 

  19. Ghoshal A, Preisegger K–H, Takayama S, Thorgeirsson SS, Snyderwine EG: Induction of mammary tumors in female Sprague–Dawley rats by the food–derived carcinogen 2–amino–1–methyl–6–phenylimidazol[4,5–b]pyridine and effect of dietary fat. Carcinogenesis 15: 2429–2433, 1994

    PubMed  Google Scholar 

  20. Cohen LA, Epstein M, Pittman B, Rivenson A: The influence of different varieties of olive oil on N–methylnitrosourea (NMU)–induced mammary tumorigenesis. Anticancer Res 20: 2307–2312, 2000

    PubMed  Google Scholar 

  21. Huggins C, Morii S, Grand LC: Mammary cancer induced by a single dose of polynuclear hydrocarbons: routes of administration. Ann Surg 154: 315–318, 1961

    PubMed  Google Scholar 

  22. Escrich E, Solanas M, Segura R: Experimental diets for the study of lipid influence on the induced mammary carcinoma in rats: I–Diet definition. In vivo 8: 1099–1106, 1994

    PubMed  Google Scholar 

  23. Escrich E, Solanas M, Ruiz de Villa MC, Ribalta T, Muntané J, Segura R: Experimental diets for the study of lipid influence on the induced mammary carcinoma in rats: II–Suitability of the diets. In vivo 8: 1107–1112, 1994

    PubMed  Google Scholar 

  24. Young S, Hallowes RC: Tumours of the mammary gland. In: Turusov V (ed.) Pathology of Tumours in Laboratory Animals, Tumours of the Rat, Vol. I. IARC Sci Publ, Lyon, 1973, pp 31–86

    Google Scholar 

  25. Dalton LW, Page DL, Dupont WD: Histologic grading of breast carcinoma. A reproducibility study. Cancer 73: 2765–2770, 1994

    PubMed  Google Scholar 

  26. Welsch CW, DeHoog JV, O'Connor DH, Sheffield LG: Influence of dietary fat levels on development and hormone responsiveness of the mouse mammary gland. Cancer Res 45: 6147–6154, 1985

    PubMed  Google Scholar 

  27. Zhang L, Bird RP, Bruce WR: Proliferative activity of murine mammary epithelium as affected by dietary fat and calcium. Cancer Res 47: 4905–4908, 1987

    PubMed  Google Scholar 

  28. Cunningham DC, Harrison LY, Shultz TD: Proliferative responses of normal human mammary and MCF–7 breast cancer cells to linoleic acid, conjugated linoleic acid and eicosanoid synthesis inhibitors in culture. Anticancer Res 17(1A): 197–203, 1997

    PubMed  Google Scholar 

  29. Snyderwine EG, Thorgeirsson UP, Venugopal M, Roberts–Thomson SJ: Mammary gland carcinogenicity of 2–amino–1–methyl–6–phenylimidazol [4,5–b]pyridine in Sprague–Dawley rats on high and low–fat diets. Nutr Cancer 31: 160–167, 1998

    PubMed  Google Scholar 

  30. Rogers AE, Conner B, Boulanger C, Lee S: Mammary tumorigenesis in rats fed diets high in lard. Lipids 21: 275–280, 1986

    PubMed  Google Scholar 

  31. Rose DP, Connolly JM: Influence of dietary linoleic acid on experimental human breast cancer cell metastasis in athymic nude mice. Int J Oncol 13: 1179–1183, 1998

    PubMed  Google Scholar 

  32. Hubbard NE, Erickson KL: Influence of dietary fats on cell populations of line 168 mouse mammary tumors: a morphometric and ultrastructural study. Cancer Lett 35: 281–294, 1987.

    Article  PubMed  Google Scholar 

  33. Rosen PP, Oberman HA: Tumors of the Mammary Gland. Atlas of Tumor Pathology (3rd series, fascicle 7). Armed Forces Institute of Pathology, Washington, DC, 1992.

  34. Innami S, Yang MG, Mickelsen O, Hafs HD: The influence of high–fat diets on estrous cycles, sperm production and fertility of rats. Proc Soc Exp Biol Med 143: 63–68, 1973

    PubMed  Google Scholar 

  35. Russo IH, Russo J: Mammary gland neoplasia in longterm rodent studies. Environ Health Persp 104: 938–967, 1996

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Cell Biology, Physiology and Immunology, Physiology Unit, Medical School, Universitat Autònoma de Barcelona, Spain

    Irmgard Costa, Raquel Moral, Montserrat Solanas & Eduard Escrich

  2. Department of Pathology, Hospital General de Granollers, Barcelona, Spain

    Irmgard Costa

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  1. Irmgard Costa
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  2. Raquel Moral
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  3. Montserrat Solanas
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  4. Eduard Escrich
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Costa, I., Moral, R., Solanas, M. et al. High-Fat Corn Oil Diet Promotes the Development of High Histologic Grade Rat DMBA-Induced Mammary Adenocarcinomas, While High Olive Oil Diet Does Not. Breast Cancer Res Treat 86, 225–235 (2004). https://doi.org/10.1023/B:BREA.0000036896.75548.0c

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  • DOI: https://doi.org/10.1023/B:BREA.0000036896.75548.0c

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