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Copper-induced oxidative stress and antioxidant defence in Arabidopsis thaliana

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Abstract

Content of reactive oxygen species (ROS): O2 \(\underline \bullet \) •, H2O2 and OH• as well as activities of antioxidant enzymes: superoxide dismutase (SOD), guaiacol peroxidase (POX) and catalase (CAT) were studied in leaves of Arabidopsis thaliana ecotype Columbia, treated with Cu excess (0, 5, 25, 30, 50, 75, 100, 150 and 300 μM). After 7 days of Cu action ROS content and the activity of SOD and POX increased, while CAT activity decreased in comparison with control. Activities of SOD, POX and CAT were correlated both with Cu concentration (0–75 μM) in the growth medium and with OH• content in leaves. Close correlation was also found between OH• content and Cu concentration. Oxidative stress in A. thaliana under Cu treatment expressed in elevated content of O2 \(\underline \bullet \)> •, H2O2 and OH• in leaves. To overcome it very active the dismutase- and peroxidase-related (and not catalase-related, as in other plants) ROS scavenging system operated in A. thaliana. Visual symptoms of phytotoxicity: chlorosis, necrosis and violet colouring of leaves as well as a reduction of shoot biomass occurred in plants.

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Authors and Affiliations

  1. Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland

    Maria Drążkiewicz, Ewa Skórzyńska-Polit & Zbigniew Krupa

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  1. Maria Drążkiewicz
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  2. Ewa Skórzyńska-Polit
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  3. Zbigniew Krupa
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Correspondence to Maria Drążkiewicz.

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Drążkiewicz, M., Skórzyńska-Polit, E. & Krupa, Z. Copper-induced oxidative stress and antioxidant defence in Arabidopsis thaliana . Biometals 17, 379–387 (2004). https://doi.org/10.1023/B:BIOM.0000029417.18154.22

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  • DOI: https://doi.org/10.1023/B:BIOM.0000029417.18154.22

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