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Albumin adsorption on Cibacron Blue F3G-A immobilized onto oligo(ethylene glycol)-terminated self-assembled monolayers

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Abstract

Self-assembled monolayers can be tailored with specific ligands to a certain protein and at the same time prevent the non-specific adsorption of other proteins. Cibacron Blue F3G-A (CB-thiol) was successfully immobilized onto tetra(ethylene glycol)-terminated alkanethiol (CB-thiol). The affinity of human serum albumin (HSA) to immobilized Cibacron Blue F3G-A was studied using mixed thiolate self-assembled monolayers on gold with different n-alkyl chain lengths and functional terminal groups (CH3-; OH- and tetra(ethylene glycol)). Surfaces were characterized using X-ray photoelectron spectroscopy and water contact angle measurements. Albumin adsorption and exchangeability of the adsorbed albumin molecules with other albumin molecules in solution were evaluated using 125I-radiolabeled HSA. Competitive adsorption between albumin and fibrinogen to the different self-assembled monolayers (SAMs) was also investigated. Results showed that the incorporation of CB-thiol on the monolayers does not increase the HSA adsorption and reversibility on the SAMs. However, although specific adsorption of HSA to the immobilized Cibacron Blue F3G-A was not demonstrated, the presence of CB-thiol decreases the affinity of fibrinogen to the OH-terminated SAMs.

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Authors and Affiliations

  1. INEB -; Instituto de Engenharia Biomédica, Laboratório de Biomateriais, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal

    Ma Cristina L. Martins & Mário A. Barbosa

  2. Departamento de Engenharia Metalúrgica e Materiais, Universidade do Porto, Faculdade de Engenharia, Porto, Portugal

    Ma Cristina L. Martins & Mário A. Barbosa

  3. Department of Bioengineering and Department of Chemical Engineering, University of Washington, Seattle, WA, 98195, USA

    Esmaeel Naeemi & Buddy D. Ratner

Authors
  1. Ma Cristina L. Martins
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  2. Esmaeel Naeemi
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  3. Buddy D. Ratner
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  4. Mário A. Barbosa
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Correspondence to Ma Cristina L. Martins.

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Martins, M.C.L., Naeemi, E., Ratner, B.D. et al. Albumin adsorption on Cibacron Blue F3G-A immobilized onto oligo(ethylene glycol)-terminated self-assembled monolayers. Journal of Materials Science: Materials in Medicine 14, 945–954 (2003). https://doi.org/10.1023/A:1026394431100

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