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Use of Isopropyl Alcohol in Histological Assays: Dehydration of Tissue, Enbessing into Paraffin, and Processing of Paraffin Sections

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Abstract

Isopropyl alcohol (isopropanol) can be used as a substitute for ethyl alcohol in tissue dehydration during embedding into paraffin and dehydration of stained sections. The use of isopropyl alcohol during paraffinization allows us to exclude treatment with intermediate solvents of paraffin (chloroform, xylene, and benzene), which reduces the degree of tissue compaction and simplifies and accelerates histological assay.

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Authors and Affiliations

  1. Laboratory of Experimental Neurocytology, Institute of Brain Research, Russian Academy of Medical Sciences, Moscow

    I. V. Viktorov & S. S. Proshin

Authors
  1. I. V. Viktorov
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  2. S. S. Proshin
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Viktorov, I.V., Proshin, S.S. Use of Isopropyl Alcohol in Histological Assays: Dehydration of Tissue, Enbessing into Paraffin, and Processing of Paraffin Sections. Bulletin of Experimental Biology and Medicine 136, 105–106 (2003). https://doi.org/10.1023/A:1026017719668

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  • DOI: https://doi.org/10.1023/A:1026017719668

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