Skip to main content
SpringerLink
Log in

Carbazole/dioxin-degrading car gene cluster is located on the chromosome of Pseudomonas stutzeri strain OM1 in a form different from the simple transposition of Tn4676

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The carbazole-degrading (car) operon on the chromosome of Pseudomonas stutzeri strain OM1 showed >99% identity to that in the 72.8 kb catabolic transposon, Tn4676, on plasmid pCAR1. Southern hybridization using probes prepared from the pCAR1 sequence and sequencing analyses showed that the OM1 chromosome contained the 55 kb DNA region, almost all of which was a part of Tn4676, flanked by two copies of novel insertion sequence, ISPst3, and included the car gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Berger B and Haas D (2001) Transposase and cointegrase: specialized transposition proteins of the bacterial insertion sequence IS21 and related elements. Cell Mol. Life Sci. 58: 403–419.

    Google Scholar 

  • Dyda F, Hickman AB, Jenkins TM, Engelman A, Craigie R, Davies DR (1994) Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases. Science 266: 1981–1986.

    Google Scholar 

  • Ka JO and Tiedje JM (1994) Integration and excision of a 2,4-dichlorophenoxyacetic acid-degradative plasmid in Alcaligenes paradoxus and evidence of its natural intergeneric transfer. J. Bacteriol. 176: 5284–5289.

    Google Scholar 

  • Maeda K, Nojiri H, Shintani M, Yoshida T, Habe H, Omori T (2003) Complete nucleotide sequence of carbazole/dioxindegrading plasmid pCAR1 in Pseudomonas resinovorans strain CA10 indicates its mosaicity and the presence of large catabolic transposon Tn4676. J. Mol. Biol. 326: 21–33.

    Google Scholar 

  • Nojiri H, Sekiguchi H, Maeda K, Urata M, Nakai S, Yoshida T, Habe H, Omori T (2001) Genetic characterization and evolutionary implications of car gene cluster in the carbazole degrader Pseudomonas sp. strain CA10. J. Bacteriol. 183: 3663–3679.

    Google Scholar 

  • Ouchiyama N, Miyachi S, Omori T (1998) Cloning and nucleotide sequence of carbazole catabolic genes from Pseudomonas stutzeri strain OM1, isolated from activated sludge. J. Gen. Appl. Microbiol. 44: 57–63.

    Google Scholar 

  • Ouchiyama N, Zhang Y, Omori T, and Kodama T (1993) Biodegradation of carbazole by Pseudomonas spp. CA06 and CA10. Biosci. Biotechnol. Biochem. 57: 455–460.

    Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular Cloning. A Laboratory Manual, 3rd edn. Cold Spring Harbour, NY: Cold Spring Harbour Laboratory Press.

    Google Scholar 

  • Sato S, Nam J-W, Kasuga K, Nojiri H, Yamane H, Omori T (1997a) Identification and characterization of genes encoding carbazole 1,9a-dioxygenase in Pseudomonas sp. strain CA10. J. Bacteriol. 179: 4850–4858.

    Google Scholar 

  • Sato S, Ouchiyama N, Kimura T, Nojiri H, Yamane H Omori T (1997b) Cloning of genes involved in carbazole degradation of Pseudomonas sp. strain CA10: nucleotide sequences of genes and characterization of meta-cleavage enzymes and hydrolase. J. Bacteriol. 179: 4841–4849.

    Google Scholar 

  • Soby S, Kirkpatrick B, Kosuge T (1993) Characterization of an insertion sequence (IS53) located within IS51 on the iaacontaining plasmid of Pseudomonas syringae pv. savastanoi. Plasmid 29: 135–141.

    Google Scholar 

  • Solinas F, Marconi AM, Ruzzi M, Zennaro E (1995) Characterization of a novel insertion sequence, IS1162, from Pseudomonas fluorescens. Gene 155: 77–82.

    Google Scholar 

  • Tsuda M, Genka H (2001) Identification and characterization of Tn4656, a novel class II transposon carrying a set of toluenedegrading genes from TOL plasmid pWW53. J. Bacteriol. 183: 6215–6224.

    Google Scholar 

  • Tsuda M, Iino T (1987) Genetic analysis of a transposon carrying toluene degrading genes on a TOL plasmid pWW0. Mol. Gen. Genet. 210: 270–276.

    Google Scholar 

  • Tsuda M, Iino T (1988) Identification and characterization of Tn4653, a transposon covering the toluene transposon Tn4651 on TOL plasmid pWW0. Mol. Gen. Genet. 213: 72–77.

    Google Scholar 

  • Yeo CC, Poh CL (1997) Characterization of IS1474, an insertion sequence of the IS21 family isolated from Pseudomonas alcaligenes NCIB9867. FEMS Microbiol. Lett. 149: 257–263.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Masaki Shintani, Hideaki Nojiri, Takako Yoshida, Hiroshi Habe & Toshio Omori

Authors
  1. Masaki Shintani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hideaki Nojiri
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takako Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroshi Habe
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Toshio Omori
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hideaki Nojiri.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shintani, M., Nojiri, H., Yoshida, T. et al. Carbazole/dioxin-degrading car gene cluster is located on the chromosome of Pseudomonas stutzeri strain OM1 in a form different from the simple transposition of Tn4676 . Biotechnology Letters 25, 1255–1261 (2003). https://doi.org/10.1023/A:1025079027730

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1025079027730

Search

Navigation