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Improving dispersed growth of Frankia using Carbopol

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Abstract

In laboratory culture, strains of the actinomycete Frankia grow slowly and form dense mycelial pellets. In this study, we have shown that by adding the anionic polymer Carbopol 941® to liquid growth media, dispersed growth of Frankia is improved. Cell protein measurements indicated higher biomass production in cultures maintained in media supplemented with Carbopol. Fluorescence staining and microscopy were used to compare viability of well-dispersed mycelia and pellets.

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Authors and Affiliations

  1. Biology Department, Fairfield University, 1073 North Benson Road, Fairfield, CT, 06824, USA

    Olivia T. Harriott

  2. Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06268, USA

    Aaron Bourret

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  1. Olivia T. Harriott
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  2. Aaron Bourret
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Correspondence to Olivia T. Harriott.

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Harriott, O.T., Bourret, A. Improving dispersed growth of Frankia using Carbopol. Plant and Soil 254, 69–74 (2003). https://doi.org/10.1023/A:1024998829163

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  • DOI: https://doi.org/10.1023/A:1024998829163

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