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Total protein measurement using a fiber-optic evanescent wave-based biosensor

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Abstract

A novel method and instrumental system to determine the total protein concentration in a liquid sample is described. It uses a fiber optic total protein sensor (FOPS) based on the principles of fiber optic evanescent wave spectroscopy. The FOPS applies a dye-immobilized porous glass coating on a multi-mode optical fiber. The evanescent waves at the fiber optic core-cladding interface are used to monitor the protein-induced changes in the sensor element. The FOPS offers a single-step method for quantifying protein concentrations without destroying the sample. The response time and reusability of the FOPS are evaluated. This unique sensing method presents a sensitive and accurate platform for the quantification of protein.

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Authors and Affiliations

  1. School of Mechanical and Production Engineering, c/o School of Mechanical and Production Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798

    P.V. Preejith, M.S. John & A. Asundi

  2. Biomedical Engineering Research Centre, c/o School of Mechanical and Production Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798

    C.S. Lim & A. Kishen

Authors
  1. P.V. Preejith
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  2. C.S. Lim
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  3. A. Kishen
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  4. M.S. John
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  5. A. Asundi
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Correspondence to C.S. Lim.

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Preejith, P., Lim, C., Kishen, A. et al. Total protein measurement using a fiber-optic evanescent wave-based biosensor. Biotechnology Letters 25, 105–110 (2003). https://doi.org/10.1023/A:1021955032291

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  • DOI: https://doi.org/10.1023/A:1021955032291

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