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Induction of a 72-kDa Heat-Shock Protein in Cultured Rat Gastric Mucosal Cells and Rat Gastric Mucosa by Zinc l-Carnosine

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Abstract

An antiulcer drug, zinc l-carnosine (polaprezinc), provides gastric mucosal protection against various irritants. In this study, we evaluated the effects of zinc l-carnosine on expression of 72-kDa heat shock protein (HSP72, stress inducible HSP70), which is known as an endogenous cytoprotectant in a wide variety of cells, including rat gastric mucosa in vitro and in vivo. Expression of HSP72 after exposure to zinc l-carnosine, zinc sulfate, or l-carnosine (1–300 μM) in rat gastric mucosal cells (RGM1) and intragastric administration of zinc l-carnosine, zinc sulfate (30 or 100 mg/kg) and l-carnosine (76 mg/kg) was investigated by western blotting and densitometric analysis. Exposure to zinc l-carnosine and zinc sulfate increased the expression of HSP72 significantly in RGM1 cells. Intragastric administration of zinc l-carnosine and zinc sulfate showed significant increment in HSP72 in rat gastric mucosa also in vivo. The ability to induce HSP72 is significantly higher in zinc l-carnosine compared with zinc sulfate based on molecular concentration in vivo. However, l-carnosine did not increase the expression of HSP72 in vitro and in vivo. Zinc derivatives, especially zinc l-carnosine, could be a strong HSP72 (chaperon) inducer, which has been known to enhance mucosal protective ability.

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Authors and Affiliations

  1. First Department of Internal Medicine, Akita University School of Medicine, Akita, Japan

    Masaru Odashima, Michiro Otaka, Mario Jin, Noriaki Konishi, Toshihiro Sato, Sayuri Kato, Tamotsu Matsuhashi, Chieko Nakamura & Sumio Watanabe

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  1. Masaru Odashima
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  2. Michiro Otaka
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  3. Mario Jin
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  4. Noriaki Konishi
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  5. Toshihiro Sato
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  6. Sayuri Kato
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  7. Tamotsu Matsuhashi
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  8. Chieko Nakamura
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  9. Sumio Watanabe
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Odashima, M., Otaka, M., Jin, M. et al. Induction of a 72-kDa Heat-Shock Protein in Cultured Rat Gastric Mucosal Cells and Rat Gastric Mucosa by Zinc l-Carnosine. Dig Dis Sci 47, 2799–2804 (2002). https://doi.org/10.1023/A:1021029927386

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