Skip to main content
SpringerLink
Log in

Apoptosis in Etiolated Wheat Seedlings: 1. Ultrastructure of the Apoptotic Cell

  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

We studied the process of apoptosis in etiolated wheat (Triticum aestivum L.) seedlings. As a result, an integral pattern of the apoptotic plant cell ultrastructure was established. In the apoptotic cells of the coleoptile, we observed chromatin condensation and margination, an increased density and specific cytoplasm fragmentation accompanied by the appearance of unusual cytoplasmic vesicles containing subcellular organelles, mitochondria in particular, in the vacuoles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Vanyushin, B.F., Apoptosis in Plants, Usp. Biol. Khim., 2001, vol. 41, pp. 3-38.

    Google Scholar 

  2. Greenberg, J.T., Programmed Cell Death: A Way of Life for Plants, Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 12094-12097.

    Google Scholar 

  3. Joza, N., Susin, S.A., Dauglas, E., Stanford, W.L., Cho, S.K., Li, C.Y.J., Sasaki, T., Ella, A.J., Cheng, H.-Y.M., Ravagnan, L., Ferri, K.F., Zamzaml, N., Wakeham, A., Hakem, R., Yoshida, H., Kong, Y.-Y., Mak, T.W., Zuniga-Pfluecker, J.C., Kroemer, G., and Penninger, J.M., Essential Role of the Mitochondrial Apoptosis-Inducing Factor in Programmed Cell Death, Nature, 2001, vol. 410, pp. 549-554.

    Google Scholar 

  4. Wang, M., Hoekstra, S., van Bergen, S., Lamers, G.E., Oppedijk, B.J., van der Heijden, M.W., de Priester, W., and Schilperoort, R.A., Apoptosis in Developing Anthers and the Role of ABA in This Process during Androgenesis in Hordeum vulgure L., Plant Mol. Biol., 1999, vol. 39, pp. 489-501.

    Google Scholar 

  5. Belhke, P.C., Lonsdale, J.E., Fath, A., Jones, R.L., Young, T.E., and Gallie, D.R., Regulation of Programmed Cell Death in Maize Endosperm by Abscisic Acid, Plant Mol. Biol., 2000, vol. 42, pp. 397-414.

    Google Scholar 

  6. Wang, M., Oppedijk, B.J., Lu, X., van Duijn, B., and Schilperoort, R.A., Apoptosis in Barley Aleurone during Germination and Its Inhibition by Abscisic Acid, Plant Mol. Biol., 1996, vol. 32, pp. 1125-1134.

    Google Scholar 

  7. Schmid, M., Simpson, D., and Gietl, C., Programmed Cell Death in Castor Bean Endosperm Is Associated with the Accumulation and Release of a Cysteine Endopeptidase from Ricinosomes, Proc. Natl. Acad. Sci. USA, 1999, vol. 96, pp. 14159-14164.

    Google Scholar 

  8. Kirnos, M.D., Aleksandrushkina, N.I., and Vanyushin, B.F., Apoptosis in the Cells of the First Leaf and Coleoptile of Wheat Seedlings: Internucleosome Fragmentation of Genome and Synthesis of “Heavy” Oligonucleosome-Size DNA Fragments, Biokhimiya, 1997, vol. 62, pp. 1008-1014.

    Google Scholar 

  9. Xu, Y. and Hanson, M.R., Programmed Cell Death during Pollination-Induced Petal Senescence in Petunia, Plant Physiol., 2000, vol. 122, pp. 1323-1333.

    Google Scholar 

  10. Gallois, P., Makishima, T., Hecht, V., Despres, B., Laudie, M., Nishimoto, T., and Cooke, R., An Arabidopsis thaliana cDNA Complementing a Hamster Apoptosis Suppressor Mutant, Plant J., 1997, vol. 11, pp. 1325-1331.

    Google Scholar 

  11. Jiang, Z.F., Zhu, S., Sun, Y.L., and Zhai, Z.H., Induction of Apoptosis in Purified Animal and Plant Nuclei by Xenopus Egg Extracts, Cell Res., 1999, vol. 9, pp. 79-90.

    Google Scholar 

  12. O'Brien, I.E., Reutelingsperger, C.P., and Holdaway, K.M., Annexin-V and TUNEL Use in Monitoring the Progression of Apoptosis in Plants, Cytometry, 1997, vol. 29, pp. 28-33.

    Google Scholar 

  13. O'Brien, I.E., Murray, B.G., Baguley, B.C., Morris, B.A., and Ferguson, I.B., Major Changes in Chromatin Condensation Suggest the Presence of an Apoptotic Pathway in Plant Cells, Exp. Cell Res., 1998, vol. 241, pp. 46-54.

    Google Scholar 

  14. Groover, A. and Jones, A.M., Tracheary Element Differentiation Uses a Novel Mechanism Coordinating Programmed Cell Death and Secondary Cell Wall Synthesis, Plant Physiol., 1999, vol. 119, pp. 375-384.

    Google Scholar 

  15. Drew, M.C., He, I.I., and Morgan, P.W., Programmed Cell Death and Aerenchyma Formation in Roots, Trends Plant Sci., 2000, vol. 5, pp. 123-127.

    Google Scholar 

  16. Fukuda, H., Programmed Cell Death of Tracheary Elements as a Paradigm in Plants, Plant Mol. Biol., 2000, vol. 44, pp. 245-253.

    Google Scholar 

  17. DeLong, A., Calderon-Urrea, A., and Dellaporta, S.L., Sex Determination Gene TASSELSEED2 of Maize Encodes a Short-Chain Alcohol Dehydrogenase Required for Stage-Specific Floral Organ Abortion, Cell, 1993, vol. 74, pp. 757-768.

    Google Scholar 

  18. Kirnos, M.D., Bakeeva, L.E., Volkova, S.A., Ganicheva, N.I., and Vanyushin, B.F., Mitochondrial Traits of Newly Formed DNA in Aging Coleoptiles of Etiolated Wheat Seedlings, Biokhimiya, 1983, vol. 48, pp. 150--1512.

    Google Scholar 

  19. Salamatova, T.S., Fiziologiya rastite'noi kletki (Physiology of the Plant Cell), Leningrad: Leningr. Gos. Univ., 1983.

    Google Scholar 

  20. James, T.N., Terasaki, F., Pavlovich, E.R., and Vikhert, A.M., Apoptosis and Pleomorphic Micromitochondriosis in the Sinus Nodes Surgically Excised from Five Patients with the Long QT Syndrome, J. Lab. Clin. Med., 1993, vol. 122, pp. 309-323.

    Google Scholar 

  21. Atlas ul'trastruktury rastitel'nykh kletok (Atlas of the Plant Cell Ultrastructure), Kozubov, G.M. and Danilova, M.F., Eds., Petrozavodsk, 1972, pp. 178-188.

  22. Bakeeva, L.E., Kirnos, M.D., Aleksandrushkina, N.I., Kazimirchyuk, S.B., Shorning, B.Yu., Zamyatnina, V.A., Yaguzhinsky, L.S., and Vanyushin, B.F., Subcellular Reorganization of Mitochondria Producing Heavy DNA in Aging Wheat Coleoptiles, FEBS Lett., 1999, vol. 457, pp. 122-125.

    Google Scholar 

  23. Kirnos, M.D., Aleksandrushkina, N.I., Shorning, B.Yu., Bubenshchikova, S.N., and Vanyushin, B.F., Superproduction of “Heavy” DNA in Nondividing Cells of Aging Wheat Seedling Coleoptiles during Apoptosis, Biokhimiya, 1997, vol. 62, pp. 1579-1589.

    Google Scholar 

  24. Kirnos, M.D., Aleksandrushkina, N.I., Bakeeva, L.E., Kazimirchuk, S.B., Shorning, B.Yu., Alekseeva, V.A., Yaguzhinskii, L.S., and Vanyushin, B.F., Unusual Fast Sedimented Mitochondria Producing “Heavy” DNA in the Cells of Aging Coleoptiles of Wheat Seedlings, Biokhimiya, 1999, vol. 64, pp. 368-380.

    Google Scholar 

  25. Lippman, R.D., The Prolongation of Life: A Comparison of Antioxidants and Geroprotectors versus Superoxide in Human Mitochondria, J. Gerontol., 1981, vol. 36, pp. 550-557.

    Google Scholar 

  26. Kirnos, M.D., Alexandrushkina, N.I., Zagorskaya, G.Ya., Kireev, I.I., and Vanyushin, B.F., Superproduction of Heavy Minicircular Mitochondrial DNA in Aging Wheat Coleoptiles, FEBS Lett., 1992, vol. 298, pp. 109-112.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Belozersky Institute of Physicochemical Biology, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia

    V. A. Zamyatnina, L. E. Bakeeva, N. I. Aleksandrushkina & B. F. Vanyushin

Authors
  1. V. A. Zamyatnina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. E. Bakeeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. I. Aleksandrushkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. F. Vanyushin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zamyatnina, V.A., Bakeeva, L.E., Aleksandrushkina, N.I. et al. Apoptosis in Etiolated Wheat Seedlings: 1. Ultrastructure of the Apoptotic Cell. Russian Journal of Plant Physiology 49, 736–745 (2002). https://doi.org/10.1023/A:1020901309679

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1020901309679

Search

Navigation