Abstract
Eight lines of grain sorghum and their F1hybrids were evaluated for contents of crude protein (CP), fat (FAT), and starch(STA); protein digestibility (PD); and in vitro dry matter disappearance (IVDMD).The effect of seed weight (SW) on these traits and the potential use of near infrared reflectance spectroscopy (NIRS) to predict them also were investigated. The male lines included three normal-seeded lines (TX2737, TX435, and P954063) and two large-seeded lines (PL-1 and Eastin1). The female lines included common U.S. seed parent lines (Wheatland, Redlan, andSA3042). The lines and their hybrids were grown under dry land conditions at Kansas State University experiment fields in Ashland and Belleville, Kansas, in 1999.The experiments were conducted using a randomized complete block design with four replications at each location. The effect of genotype was significant for all measured traits. The male parent lines were highly variable and expressed high levels of genetic variation in combining ability for CP, PD, STA, and SW. The female parents were genetically more uniform; however, significant general combining ability effects were noted for PD and SW. Significant negative correlations were noted between CP and STA and between SW and STA. Significant positive correlations were found between CP and SW and between FAT and IVDMD. Crude protein content was predicted accurately by NIRS. Fat content and IVDMD could not be predicted by NIRS. The NIRS equations based on ground samples were more accurate than those based on whole-seed samples.
Similar content being viewed by others
References
Association of Official Analytical Chemists, 1990. Official Methods of Analysis. 16th edn. AOAC, Arlington, VA.
Axtell, J.D., A.W. Kirleis, M.M. Hassen, N.D. Mason, E.T. Mertz & L. Munck, 1981. Digestibility of Sorghum Proteins. Proc Natl Acad Sci USA 78: 1333–1335.
Beil, G.M. & R.E. Atkins, 1967. Estimates of general and specific combining ability in F1 hybrids for grain yield and its components in grain sorghum, Sorghum vulgare Pers. Crop Sci 7: 225–228.
Bramel-Cox, P.J., M.A. Lauver & M.E. Witt, 1990. Potential gain from selection in grain sorghum for higher protein digestibility. Crop Sci 30: 521–524.
Buffo, A.R., C.L. Weller & A. Parkhurt, 1998. Relationship among grain sorghum quality factors. Cereal Chem 75(1): 100–104.
Comstock, R.E. & H.F. Robinson, 1952. Estimation of average dominance of genes. In: J.W. Gowen (Ed.), Heterosis, pp. 494–516. Iowa State College Press, Ames, IA.
Cox, D.J. & K.J. Frey, 1984. Combining ability and the selection of parents for interspecific oat matings. Crop Sci 24: 963–967.
Hallauer, A.R. & J.B. Miranda, 1988. Quantitative Genetics in Maize Breeding. 2nd edn. Iowa State University Press, Ames, IA.
Hamaker, B.R., A.W. Kirleis, L.G. Butler, J.D. Axtell & E.T. Mertz, 1987. Improving the in vitro protein digestibility of sorghum with reducing agents. Proc Natl Acad Sci USA 84: 626–628.
Hamaker, B.R., A.W. Kirleis, E.T. Mertz & J.D. Axtell, 1986. Effect of cooking on the protein profiles and in vitro digestibility of sorghum and maize. J Agric Food Chem 34: 647–649.
Hibberd, C.A., R.L. Hintz & D.G.Wagner, 1980. The Effect of Location on the Nutritive Characteristics of Several Grain Sorghum Hybrids. Anim Sci Research Report. Agric Exp Station. Oklahoma State Univ. and USDA-SEA-AR.
Hibberd, C.A., E.D. Mitchell, D.G. Wagner & R.L. Hintz, 1979. Seed Characteristics of Different Varieties of Grain Sorghum. Anim Sci Research Report, MP-104. Agric Exp Station. Oklahoma State Univ. and USDA-SEA-AR.
Hibberd, C.A., D.G. Wagner, R.L. Hintz & D.D. Griffin, 1983. Effect of Sorghum Grain Variety and Processing Method on the Site and Extent of Starch Digestion in Steers. Agric Exp Station. Oklahoma State Univ. and USDA-SEA-AR. Anim Sci Res Report. MP-114.
Hibberd, C.A., D.G. Wagner, R.L. Schemm, E.D. Mitchell, Jr., R.L. Hintz & D.E. Weibel, 1982. Nutritive characteristics of different varieties of sorghum and corn grains. J Anim Sci 55: 665–672.
Kansas Grain Sorghum Commission, 1997. Kansas grain sorghum quality study. www.ksgrains.com/sorghum
Keating, E.K., W.J., Saba, W.H. Hale & B. Taylor, 1965. Further observations on the digestion of milo and barley by steers and lambs. J Anim Sci 34: 1079.
Liang, G.H.L., T.L. Walter, C.D. Nickell, & Y.O. Koh, 1969. Heritability estimates and interrelationships among agronomic traits in grain sorghum, Sorghum bicolor (L.) Moench. Can J Genet Cytol 11: 199–208.
Martens, H. & T. Naes, 1989. Multivariate Calibrations. John Wiley and Sons, Guilford, UK.
Mertz, E.T., M.M. Hassen, C. Cairns-Whitten, A.W. Kirleis, L. Tu & J.D. Axtell, 1984. Pepsin digestibility of proteins in sorghum and other major cereals. Proc Natl Acad Sci USA 81: 1–2.
Oria, M.P., B.R. Hamaker & J.M. Schull, 1995. In vitro pepsin digestibility of developing and mature sorghum in grain in relation to α, β, and γ-kafirin disulfide crosslinking. J Cereal Sci 22: 85–93.
Pazdernik, D.L., A.S. Killam & J.H. Orf, 1997. Analysis of amino and fatty acid composition in soybean seed, using near infrared reflectance spectroscopy. Agron J 89: 679–685.
Pedersen, J.F., T. Milton & R.A. Mass, 2000. A twelve-hour in vitro procedure for sorghum grain feed quality assessment. Crop Sci 40: 204–208.
Rooney, L.W. & R.L. Pflugfelder, 1986. Factors affecting starch digestibility with special emphasis on sorghum and corn. J Anim Sci 63: 1607–1623.
Saba, W.J., W.H. Hale, F. Hubbert Jr., J. Kiernat & B. Taylor, 1964. Digestion of milo and barley by cattle. J Anim Sci 23: 533–536.
SAS Institute, 1990. SAS/STAT User's Guide. Version 6. 4th edn. Vol. SAS Inst., Cary, NC.
Sastry, L.V.S., J.W. Paulis, J.A. Bietz & J.S. Wall, 1986. Genetic variation of storage proteins in sorghum grain: Studies by isoelectric focusing and high-performance liquid chromatography. Cer Chem 63: 420–427.
Schake, L.M., A. Driedger, J.K. Riggs & D.N. Clamme, 1976. Corn and grain sorghum evaluation for beef cattle. J Anim Sci 43: 959–965.
Sepel, N.A. & O.F. Sepel, 1970. The effect of hybridization and external conditions on grain yield and quality in sorghum. Plant Breeding Abstr 40: 895.
Streeter, M.N., G.M. Hill, D.G. Wagner, C.A. Hibberd & F.N. Owens, 1990. The effect of sorghum grain variety on site and extent of digestion in beef heifers. J Anim Sci 68: 1121–1132.
Streeter, M.N., G.M. Hill, D.G. Wagner, C.A. Hibberd & F.N. Owens, 1993. Chemical and physical properties and in vitro dry matter and starch digestion of eight sorghum grain hybrids and maize. Anim Feed Sci and Techn 44: 45–58.
Van Barneveld, R.J., J.D. Nuttall, P.C. Flinn & B.G. Osborne, 1999. Near infrared reflectance measurement of digestible energy content of cereals for growing pigs. J Near Infrared Spectrosc 7: 1–7.
Weaver, C.A., B.R. Hamaker & J.D. Axtell, 1998. Discovery of grain sorghum germplasm with high uncooked and cooked in vitro protein digestibilities. Cereal Chem 75: 665–670.
Wester, T.J., S.M. Gramlich, R.A. Britton, & R.A. Stock, 1992. Effect of grain sorghum hybrid on in vitro rate of starch disappearance and finishing performance of ruminants. J Anim Sci 70: 2866–2876.
Williams, P.C., K.R. Preston, K.H. Norris & P.M. Starkey, 1984. Determination of amino acids in wheat and barley by near-infrared reflectance spectroscopy. J Food Sci 49: 17–20.
Williams, P.C. & D.C. Sobering, 1993. Comparison of commercial near infrared transmittance and reflectance instruments for analysis of whole grains and seeds. J Near Infrared Spectrosc 1: 25–32.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hicks, C., Tuinstra, M., Pedersen, J. et al. Genetic analysis of feed quality and seed weight of sorghum inbred lines and hybrids using analytical methods and NIRS. Euphytica 127, 31–40 (2002). https://doi.org/10.1023/A:1019943805514
Issue Date:
DOI: https://doi.org/10.1023/A:1019943805514