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Regulation of alternative oxidase gene expression in soybean

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Abstract

Soybean (Glycine max cv. Stevens) suspension cells were used to investigate the expression of the alternative oxidase (Aox) multigene family. Suspension cells displayed very high rates of cyanide-insensitive respiration, but Aox3 was the only isoform detected in untreated cells. Incubation with antimycin A, citrate, salicylic acid or at low temperature (10 °C) specifically induced the accumulation of the Aox1 isoform. Aox2 was not observed under any conditions in the cells. Increases in Aox1 protein correlated with increases in Aox1 mRNA. Treatment of soybean cotyledons with norflurazon also induced expression of Aox1. Reactive oxygen species (ROS) were detected upon incubation of cells with antimycin, salicylic acid or at low temperature, but not during incubation with citrate. Aox1 induction by citrate, but not by antimycin, was prevented by including the protein kinase inhibitor staurosporine in the medium. The results suggest that multiple pathways exist in soybean to regulate expression of Aox genes and that Aox1 specifically is induced by a variety of stress and metabolic conditions via at least two independent signal transduction pathways.

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Authors and Affiliations

  1. Plant Molecular Biology Group, Biochemistry and Molecular Biology, School of Biomedical and Chemical Sciences, University of Western Australia, 35 Stirling Highway, Crawley, 6009, WA, Australia

    Ira Djajanegara, Christel Mathieu, Tulene McCabe, James Whelan & David A. Day

  2. School of Science, Food and Horticulture, College of Science, Technology and Environment, University of Western Sydney, Locked Bag 1797, Penrith South, DC, 1797, NSW, Australia

    Patrick M. Finnegan

Authors
  1. Ira Djajanegara
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  2. Patrick M. Finnegan
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  3. Christel Mathieu
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  4. Tulene McCabe
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  5. James Whelan
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  6. David A. Day
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Djajanegara, I., Finnegan, P.M., Mathieu, C. et al. Regulation of alternative oxidase gene expression in soybean. Plant Mol Biol 50, 735–742 (2002). https://doi.org/10.1023/A:1019942720636

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