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A Heterogeneously Structured Composite Based on Poly(lactic-co-glycolic acid) Microspheres and Poly(vinyl alcohol) Hydrogel Nanoparticles for Long-Term Protein Drug Delivery

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Abstract

Purpose. To prepare a heterogeneously structured composite based on poly (lactic-co-glycolic acid) (PLGA) microspheres and poly(vinyl alcohol) (PVA) hydrogel nanoparticles for long-term protein drug delivery.

Methods. A heterogeneously structured composite in the form of PLGA microspheres containing PVA nanoparticles was prepared and named as PLGA-PVA composite microspheres. A model protein drug, bovine serum albumin (BSA), was encapsulated in the PVA nanoparticles first. The BSA-containing PVA nanoparticles was then loaded in the PLGA microspheres by using a phase separation method. The protein-containing PLGA-PVA composite microspheres were characterized with regard to morphology, size and size distribution, BSA loading efficiency, in vitroBSA release, and BSA stability.

Results. The protein-containing PLGA-PVA composite microspheres possessed spherical shape and nonporous surface. The PLGA-PVA composite microspheres had normal or Gaussian size distribution. The particle size ranged from 71.5 μm to 282.7 μm. The average diameter of the composite microspheres was 180 μm. The PLGA-PVA composite microspheres could release the protein (BSA) for two months. The protein stability study showed that BSA was protected during the composite microsphere preparation and stabilized inside the PLGA-PVA composite microspheres.

Conclusions. The protein-containing PLGA-PVA composite may be suitable for long-term protein drug delivery.

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Author notes

  1. Nuo Wang

    Present address: Mutual Pharmaceutical Company, Inc., 1100 Orthodox Street, Philadelphia, Pennsylvania, 19124

  2. Jia Kui Li

    Present address: Pharmaceutical R&D, Hoffmann-La Roche, 340 Kingsland St., Nutley, New Jersey, 07110

Authors and Affiliations

  1. Division of Pharmaceutics and Industrial Pharmacy, Arnold and Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, Brooklyn, New York, 11201

    Nuo Wang

  2. Synthon, BV, Microweg 22, 6545 CM, Nijmegen, The Netherlands. To whom correspondence should be addressed

    Xue Shen Wu

Authors
  1. Nuo Wang
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Correspondence to Xue Shen Wu.

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Wang, N., Wu, X.S. & Li, J.K. A Heterogeneously Structured Composite Based on Poly(lactic-co-glycolic acid) Microspheres and Poly(vinyl alcohol) Hydrogel Nanoparticles for Long-Term Protein Drug Delivery. Pharm Res 16, 1430–1435 (1999). https://doi.org/10.1023/A:1018911411381

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