Skip to main content
Log in

Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Silkworm hemolymph when added at 5% (v/v) to medium increased the production of recombinant β-galactosidase in Spodoptera frugipera/ Autographa californica nuclear polyhedrosis virus (AcNPV) system by 4.5-fold. Silkworm hemolymph also increased the host cell longevity by two-fold. Viability of the host cell was maintained at a constant level for 6 days after baculovirus infection in the medium containing 5% silkworm hemolymph, while host cells began to die 3 days after infection in the medium without hemolymph.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ha, SH, Park, TH and Kim, S-E (1996). Biotechnol. Tech. 10:401-406

    Google Scholar 

  • Hara, T, Tarui, H, Kawaguchi, H, Yoshimura, H and Kawarabata, T (1995). J. Seric. Sci. Jpn. 64:477-481

    Google Scholar 

  • Lee, SH and Park, TH (1994). Biotechnol. Lett. 16:327-332

    Google Scholar 

  • Lee, SH, and Park, TH. (1995). Biotechnol. Tech. 9:719-724

    Google Scholar 

  • Licari, P, Jarvis, DL and Bailey, JE (1993). Biotechnol. Prog. 9:146-152

    Google Scholar 

  • Lindsay, DA and Betenbaugh, MJ (1992). Biotechnol. Bioeng. 39:614-618

    Google Scholar 

  • Maiorella, B, Inlow, D, Shauger, A and Harano, D (1988). Bio/technol. 6:1406-1410

    Google Scholar 

  • Miller, JH (1972). Purification of β-galactosidase. In: Experiments in Molecular Genetics, pp 398-404, New York: Cold Spring Harbor Laboratory Press

    Google Scholar 

  • Miltenburger, HG (1982). Investigation on the cultivation of insect cell lines in serum-free media. In: Hormonally Defined Media: A Tool in Cell Biology, G Fisher and RJ Wieser, eds pp 31-43, New York: Springer-Verlag

    Google Scholar 

  • Murhammer, DW and Goochee, CF (1988). Bio/technol. 6:1411-1418

    Google Scholar 

  • Reuveny, S, Kim, YJ, Kemp, CW and Shiloach, J (1993). Biotechnol. Bioeng. 42:235-239

    Google Scholar 

  • Wang, M-Y, Kwong, S and Bentley, WE (1993). Biotechnol. Prog. 9:355-361

    Google Scholar 

  • Wickham, TJ, Davis, T, Granados, RR, Shuler, ML and Wood, HA (1992). Biotechnol. Prog. 8:391-396

    Google Scholar 

  • Wilkie, GEI, Stockdale, H and Pirt, SV (1980). Develop. Biol. Stand. 46:29-37

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ha, S., Park, T. Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph. Biotechnology Letters 19, 1087–1091 (1997). https://doi.org/10.1023/A:1018484309194

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018484309194

Keywords

Navigation