Abstract
Manganese and lignin peroxidase (MnP, LiP) activities were measured in straw extracts from cultures of Phanerochaete chrysosporium. Out of six MnP substrates, the MBTH/DMAB (3-methyl-2-benzothiazolinone hydrazone/3-(dimethylamino)benzoic acid), gave the highest MnP activity. Detection of LiP activity as veratryl alcohol oxidation was inhibited by phenols in the straw culture extracts. Appropriate levels of veratryl alcohol and peroxide (4 mM and 0.4 mM, respectively), and a restricted sample volume (not larger than 10%) were necessary to detect activity.
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del Pilar Castillo, M., Ander, P. & Stenstrom, J. Lignin and manganese peroxidase activity in extracts from straw solid substrate fermentations. Biotechnology Techniques 11, 701–706 (1997). https://doi.org/10.1023/A:1018423829918
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DOI: https://doi.org/10.1023/A:1018423829918