Skip to main content
SpringerLink
Log in

Fluorescence Characteristics of Variously Charged Asymmetric Monomethine Cyanine Dyes in the Presence of Nucleic Acids

  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

Three asymmetric monomethine cyanine dyes bearing one, two, and three positive charges have been synthesized, and their absorption and fluorescence characteristics in the presence of nucleic acids were studied. The maxima of their longest wavelength absorption band lie between 500 and 520 nm. The dyes do not show fluorescence of their own in TE buffer (pH = 7.5), but become strongly fluorescent (QF = 0.2–0.6) on binding to double-stranded DNA. The fluorescence maxima of the investigated dye-dsDNA complexes are in the region of 530–550 nm. The influence of the dye/DNA ratio on both the position and intensity of the fluorescence maxima of the complexes is investigated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. G. Cosa, K.-S. Focsaneanu, J. R. N. McLean, J. P. McNamee, and J. C. Scaiano (2001) Photochem. Photobiol. 73, 585–599.

    Google Scholar 

  2. H. S. Rye, S. Yue, D. E. Wemmer, M. A. Quesada, R. P. Haugland, R. A. Mathies, and A. N. Glazer (1992) Nucleic Acids Res. 20, 2803–2812.

    Google Scholar 

  3. S. Prodhomme, J. P Demaret, S. Vinogradov, U. Asseline, L. Morin-Allory, and P. Vigny (1999) J. Photochem. Photobiol. B: Biol. 53, 60–69.

    Google Scholar 

  4. I. Timtcheva, V. Maximova, T. Deligeorgiev, N. Gadjev, R. Sabnis, and I. Ivanov (1997) FEBS Lett. 405, 141–144.

    Google Scholar 

  5. I. Timtcheva, V. Maximova, T. Deligeorgiev, D. Zaneva, and I. Ivanov (2000) J. Photochem. Photobiol. A: Chem. 130, 7–11.

    Google Scholar 

  6. I. Timtcheva, V. Maximova, T. Deligeorgiev, N. Gadjev, K. H. Drexhage, and I. Petkova (2000) J. Photochem. Photobiol. B: Biol. 58, 130–135.

    Google Scholar 

  7. N. I. Gadjev, T. G. Deligeorgiev, and S. H. Kim (1999) Dyes and try Pigments 40, 181–186.

    Google Scholar 

  8. T. Deligeorgiev, N. Gadjev, K.-H. Drexhage, and R. Sabnis (1995) Dyes and Pigments, 29, 315–322.

    Google Scholar 

  9. R. P. Haugland (1992) in Molecular Probes: Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes, Eugene, OR

    Google Scholar 

  10. J. B. Chaires, N. Dattagupta, and D. M. Crothers (1982) Biochemistry 21, 3933–3940.

    Google Scholar 

  11. K. G. Strothkamp, R. E. Strothkamp (1994) J. Chem. Educ. 71, 1.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Organic Chemical Technology, Faculty of Chemistry, University of Sofia, 1, James Bouchier Avenue, 1126, Sofia, Bulgaria

    Todor Deligeorgiev

  2. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Ilijana Timtcheva

  3. Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Vera Maximova

  4. University of Sofia, 1, James Bouchier Avenue, 1126, Sofia, Bulgaria

    Nikolai Gadjev

  5. Department of Physical Chemistry, University of Siegen, D-57068, Siegen, Germany

    Karl-Heinz Drexhage

Authors
  1. Todor Deligeorgiev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ilijana Timtcheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vera Maximova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nikolai Gadjev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Karl-Heinz Drexhage
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Todor Deligeorgiev.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Deligeorgiev, T., Timtcheva, I., Maximova, V. et al. Fluorescence Characteristics of Variously Charged Asymmetric Monomethine Cyanine Dyes in the Presence of Nucleic Acids. Journal of Fluorescence 12, 225–229 (2002). https://doi.org/10.1023/A:1016817018665

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1016817018665

Search

Navigation