Skip to main content
Log in

Collection of Arabidopsis thalianaMutants with Altered Sensitivity to Oxidative Stress Inductors

  • Published:
Biology Bulletin of the Russian Academy of Sciences Aims and scope Submit manuscript

Abstract

Selective systems for screening Arabidopsis thaliana(L.) Heynh. mutants with altered sensitivity to the oxidative stress (OS) inductors norflurazon (NF), acifluorfen (AF), and plumbagin (PB) were developed and a collection of 28 mutants was obtained. Dwarf and necrotic forms predominated among the NF-tolerant mutants, while pigment mutants and those with changed root morphology prevailed among the AF-tolerant and PB-sensitive mutants, respectively. Genetic and biochemical analysis of certain mutants was performed; quantitative and qualitative changes in the content of superoxide dismutase and peroxidase isoforms have been revealed. These data, complemented by the data on the cross-tolerance (sensitivity) of the mutants to paraquat, indicate a correlation between tolerance to the OS inductors and the functions of antioxidant systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  • Beyer, W.F. andFridovich, I., Assaying for Superoxide Dismutase Activity: Some Large Consequences of Minor Changes in Conditions, Anal. Biochem., 1987, vol. 161, pp. 559-566.

    Google Scholar 

  • Brigham, Z.A.,Michaels, P.J., andFlores, H.E., Cell-Specific Production and Antimicrobial Activity of Naphthoquinones in Roots of Lithospermum erythrorhizon, Plant Physiol., 1999, vol. 119, pp. 417-428.

    Google Scholar 

  • Deitrich, R.A.,Delaney, T.P.,Uknes, S.J., et al., Arabidopsis Mutants Simulating Disease Resistance Response, Cell, 1994, vol. 77, pp. 565-577.

    Google Scholar 

  • Donahue, J.L.,Okpodu, C.M.,Cramer, C.L., et al., Responses of Antioxidants to Paraquat in Pea Leaves, Plant Physiol., 1997, vol. 113, pp. 249-257.

    Google Scholar 

  • Duke, S.O.,Becerril, J.M.,Sherman, T.D., et al., Protoporphirinogen Oxidase-Inhibiting Herbicides, Weed Sci., 1991, vol. 39, pp. 465-473.

    Google Scholar 

  • Ezhova, T.A.,Ondar, U.N.,Soldatova, O.P., andMamanova,L.B., Genetic and Physiological Studies of Dwarf Mutants of Arabidopsis thaliana (L.) Heynh., Ontogenez, 1997a, vol. 28, no. 5, pp. 344-351.

    Google Scholar 

  • Ezhova, T.A.,Soldatova, O.P.,Ondar, U.N., et al., Dwarf Mutants of Arabidopsis thaliana (L.) Heynh. Tolerant to Nor-flurazone as Objects of Studying Resistance against Oxidative Stress, Fiziol. Rast., 1997b, vol. 44, no. 5, pp. 665-670.

    Google Scholar 

  • Ezhova, T.A.,Soldatova, O.P.,Mamanova, L.B., et al., A Study of the Activity of Enzymes of the Antioxidant System in Ontogenesis of Arabidopsis thaliana Mutants Tolerant to Norflurazone, Ontogenez, 2000, vol. 31, no. 1, pp. 1-7.

    Google Scholar 

  • Fedtke, K., Biokhimiya i fiziologiya deistviya gerbitsidov (Biochemistry and Physiology of Herbicide Action), Moscow: Agropromizdat, 1985.

    Google Scholar 

  • Foy, C.D.,Chaney, R.L., andWhite, M.C., The Physiology of Metal Toxicity in Plants, Annu. Rev. Plant Physiol., 1978, vol. 29, pp. 511-566.

    Google Scholar 

  • Gol'dfel'd, M.G. andKarapetyan, N.V., Physicochemical Bases of Herbicides Action, Itogi Nauki Tekhn. Ser. Biol. Khim., Moscow: VINITI, 1989, vol. 30.

    Google Scholar 

  • Haughn, G.M. andSomerville, C.P., Sulfonurea-Resistant Mutants of Arabidopsis thaliana, Mol. Gen. Genet., 1986, vol. 204, pp. 430-434.

    Google Scholar 

  • Howden, R.,Andersen, C.R.,Goldsbrough, P.B., andCobbett, C.S., A Cadmium-Sensitive, Glutathion-Deficient Mutant of Arabidopsis thaliana, Plant Physiol., 1995, vol. 107, pp. 1067-1073.

    Google Scholar 

  • Jones, R.L., The Control of Plant Cell Elongation by Aux in and Gibberellin, Plant Growth Substances, Bopp, M., Ed., Berlin: Springer, 1985, pp. 275-283.

    Google Scholar 

  • Kliebenstein, D.J.,Monde, R.-A., andLast, R.L., Superoxide Dismutase in Arabidopsis, an Eclectic Enzyme Family with Disparate Regulation and Protein Localization, Plant Physiol., 1998, vol. 118, pp. 637-650.

    Google Scholar 

  • Kof, E.M., Plant Growth and Activity of Phytohormones in the Context of Mutant Genes Action, Doct. Sci. (Biol.) Dissertation, Kiev: Institute of Plant Physiology and Genetics Akad. Nauk USSR, 1991.

    Google Scholar 

  • Kvitko, K.V., Aceptic Culture of Arabidopsis thaliana (L.) Heynh. and Its Prospective Use in Botanical Studies, Vestn. LGU, 1960, vol. 15, no. 3, pp. 47-56.

    Google Scholar 

  • Larsen, P.B.,Tai, C.-Y.,Kochian, L.V., andHowell, S.H., Arabidopsis Mutants with Increased Sensitivity to Aluminium, Plant Physiol., 1996, vol. 110, pp. 743-751.

    Google Scholar 

  • Pinhero, R.G.,Rao, M.K.,Paliath, G., et al., Changes in Activities of Antioxidant Enzymes and Their Relationship to Genetic and Paclobutrazol-Induced Chiling Tolerance of Maize Seedling, Plant Physiol., 1997, vol. 114, pp. 695-704.

    Google Scholar 

  • Soldatova, O.P.,Ezhova, T.A.,Ondar, U.N., et al., Arabidopsis thaliana (L.) Heynh. Mutants Tolerant to the Inhibitor of Carotenoid Biosynthesis Norflurazone, Genetika, 1996, vol. 32, no. 7, pp. 956-961.

    Google Scholar 

  • Tanaka, K., Gene Structures and Expression Control of Active Oxygen Scavenging Enzymes in Rice, Stress Responses of Photosynthetic Organisms, Saton, K. andMurata, N., Eds., Amsterdam: Elsevier, 1998, pp. 53-68.

    Google Scholar 

  • Yanushkevich, S.I., Ispol'zovanie arabidopsis v prakticheskikh zanyatiyakh po obshchei genetike (The Use of Arabidopsis in Practical Studies on General Genetics), Moscow: Mosk. Gos. Univ., 1985.

    Google Scholar 

  • Zeevaart, J.A.D. andCreelman, R.A., Metabolism and Physiology of Abscisic Acid, Annu. Rev. Plant Physiol. Plant Molec. Biol., 1988, vol. 39, pp. 439-531.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ezhova, T.A., Soldatova, O.P., Mamanova, L.B. et al. Collection of Arabidopsis thalianaMutants with Altered Sensitivity to Oxidative Stress Inductors. Biology Bulletin 28, 449–458 (2001). https://doi.org/10.1023/A:1016736025409

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1016736025409

Keywords

Navigation