Abstract
The development of etiolated wheat (Triticum aestivum L.) seedlings is necessarily accompanied by apoptosis in their coleoptiles and first leaves. Internucleosome DNA fragmentation, which is characteristic of apoptosis, was detected in the coleoptile as soon as six days after germination. After eight days of germination, DNA fragmentation was clearly expressed in the coleoptile and was noticeable in the apical part of the first-leaf blade. Growing of intact seedlings or incubation of their shoots in the presence of such phytohormones as benzyladenine, gibberellin A3, fusicoccin C, and 2,4-D at the concentration of 10–5 M did not essentially affect DNA fragmentation in the coleoptile. As distinct from antioxidants, none of the phytohormones used prevented apoptosis in wheat seedlings. In contrast, ABA (10–5 M) and an ethylene producer, ethrel (2-chloroethylphosphonic acid, 10–2–10–3 M), stimulated sharply DNA fragmentation in the coleoptile. An inhibitor of DNA methylation, 5-azacytidine, was very efficient in the stimulation of DNA fragmentation in the coleoptiles of eight-day-old seedlings at its concentration of 100 μg/ml. Thus, some phytohormones can regulate apoptosis, and DNA methylation is involved in this process. Our results indicate that apoptosis activation by some phytohormones may be mediated by their regulation of DNA methylation/demethylation, which is responsible for the induction of genes encoding apoptogenic proteins and/or the repression of antiapoptotic genes.
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REFERENCES
Vanyushin, B.F., Apoptosis of Plants, Usp. Biol. Khim., 2001, vol. 41, pp. 3–38.
Skulachev, V.P., The Programmed Death Phenomena, Aging, and the Samurai Law of Biology, Exp. Gerontol., 2001, vol. 36, pp. 995–1024.
Vanyushin, B.F. and Berdyshev, G.D., Molekulyarno-geneticheskie mekhanizmy stareniya (Molecular and Genetic Mechanisms of Aging), Moscow: Meditsyna, 1977.
Chailakhyan, M.Kh., Regulyatsiya tsveteniya vysshikh rastenii (Control of the Higher Plant Flowering), Moscow: Nauka, 1988.
Greenberg, J.T., Programmed Cell Death: A Way of Life for Plants, Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 12094–12097.
Kirnos, M.D., Aleksandrushkina, N.I., and Vanyushin, B.F., Apoptosis in the Cells of the First Leaf and Coleoptile in Wheat Seedlings: Internucleosomal DNA Fragmentation and the Synthesis of “Heavy” Oligonucleosome Fragments of DNA, Biokhimiya, 1997, vol. 62, pp. 1008–1014.
Kirnos, M.D., Aleksandrushkina, N.I., Shorning, B.Yu., Bubenshchikova, S.N., and Vanyushin, B.F., Overproduction of “Heavy” DNA in Non-Dividing Cells of the Aging Coleoptile in Wheat Seedlings during Apoptosis, Biokhimiya, 1997, vol. 62, pp. 1579–1589.
Kirnos, M.D., Aleksandrushkina, N.I., Shorning, B.Yu., Kudryashova, I.B., and Vanyushin, B.F., Internucleosomal Fragmentation and DNA Synthesis in Wheat Seedlings, Fiziol. Rast. (Moscow), 1999, vol. 46, pp. 48–57 (Russ. J. Plant Physiol., Engl. Transl.).
Bakeeva, L.E., Kirnos, M.D., Aleksandrushkina, N.I., Kazimirchuk, S.B., Shorning, B.Yu., Zamyatnina, V.A., Yaguzhinsky, L.S., and Vanyushin, B.F., Subcellular Reorganization of Mitochondria Producing Heavy DNA in Aging Wheat Coleoptiles, FEBS Lett., 1999, vol. 457, pp. 122–125.
Bakeeva, L.E., Zamyatnina, V.A., Shorning, B.Yu., Aleksandrushkina, N.I., and Vanyushin, B.F., The Effect of Ionol (BHT) as Antioxidant on the Growth and Development of Etiolated Wheat Seedlings: Control of Apoptosis, Cell Division, Organelle Ultrastructure, and Plastid Differentiation, Biokhimiya, 2001, vol. 66, pp. 1048–1059.
Wang, M., Oppedijk, B.J., Lu, X., van Duijn, B., and Schilperoort, R.A., Apoptosis in Barley Aleurone during Germination and Its Inhibition by Abscisic Acid, Plant Mol. Biol., 1996, vol. 32, pp. 1125–1134.
Bethke, P.C., Lonsdale, J.E., Fath, A., Jones, R.L., Young, T.E., and Gallie, D.R., Regulation of Programmed Cell Death in Maize Endosperm by Abscisic Acid, Plant Mol. Biol., 2000, vol. 42, pp. 397–414.
Liljeroth, E. and Bryngelsson, T., DNA Fragmentation in Cereal Roots Indicative of Programmed Root Cortical Cell Death, Physiol. Plant., 2001, vol. 111, pp. 365–372.
Wang, M., Hoekstra, S., van Bergen, S., Lamers, G.E., Oppedijk, B.J., van der Heijden, M.W., de Priester, W., and Schilperoort, R.A., Apoptosis in Developing Anthers and the Role of ABA in This Process during Androgenesis in Hordeum vulgare L., Plant Mol. Biol., 1999, vol. 39, pp. 489–501.
Xu, Y. and Hanson, M.R., Programmed Cell Death during Pollination-Induced Petal Senescence in Petunia, Plant Physiol., 2000, vol. 122, pp. 1323–1333.
Gunawardena, A.H.L.A.N., Pearce, D.M., Jackson, M.B., Hawes, C.R., and Evans, D.E., Characterisation of Programmed Cell Death during Aerenchyma Formation Induced by Ethylene or Hypoxia in Roots of Maize (Zea mays L.), Planta, 2001, vol. 212, pp. 205–214.
Fukuda, H., Programmed Cell Death of Tracheary Elements as a Paradigm in Plants, Plant Mol. Biol., 2000, vol. 44, pp. 245–253.
Jabs, T., Reactive Oxygen Intermediates as Mediators of Programmed Cell Death in Plants and Animals, Biochem. Pharmacol., 1999, vol. 57, pp. 231–245.
Kirnos, M.D., Aleksandrushkina, N.I., Zagorskaya, G.Ya., Kireev, I.I., and Vanyushin, B.F., Superproduction of Heavy Minicircular Mitochondrial DNA in Aging Wheat Coleoptiles, FEBS Lett., 1992, vol. 298, pp. 109–112.
Yen, C.H. and Yang, C.H., Evidence for Programmed Cell Death during Leaf Senescence in Plants, Plant Cell Physiol., 1998, vol. 39, pp. 922–927.
Shorning, B.Yu., Smirnova, E.G., Yaguzhinsky, L.S., and Vanyushin, B.F., Requirement in the Superoxide Formation for the Development of Wheat Seedlings, Biokhimiya, 2000, vol. 65, pp. 1612–1617.
Kulaeva, O.N., Tsitokininy, ikh structura i funktsiya (Cytokinins: Their Structure and Functions), Moscow: Nauka, 1973.
Zhou, J., Zhuo, H.Z., and Dai, Y.R., Effect of Ethrel on Apoptosis in Carrot Protoplasts, Plant Growth Regul., 1999, vol. 27, pp. 119–123.
Young, T.E. and Gallie, D.R., Analysis of Programmed Cell Death in Wheat Endosperm Reveals Differences in Endosperm Development between Cereals, Plant Mol. Biol., 2000, vol. 42, pp. 397–414.
Vanyushin, B.F., Replicative DNA Methylation in Animals and Higher Plants, Curr. Top. Microbiol. Immunol., 1984, vol. 108, pp. 99–114.
Vanyushin, B.F. and Kirnos, M.D., DNA Methylation in Plants, Gene, 1988, vol. 74, pp. 117–121.
Kirnos, M.D., Aleksandrushkina, N.I., Vlasova, T.I., and Vanyushin, B.F., Structural and Functional Arrangement of Methylation in Replicated Genome, Mol. Biol. (Moscow), 1995, vol. 29, pp. 1242–1257.
Kirnos, M.D., Artyukhovskaya, N.A., Aleksandrushkina, N.I., Ashapkin, V.V., and Vanyushin, B.F., Phytohormone Effect on the Nuclear DNA Methylation at Replication and Post-Replication in the S-Phase of the Cell Cycle in the First Leaf of Etiolated Wheat Seedlings, Biokhimiya, 1986, vol. 51, pp. 1875–1885.
Jackson-Grusby, L., Beard, C., Possemato, R., Tudor, M., Fambrough, D., Csankovszki, G., Dausman, J., Lee, P., Wilson, C., Lander, E., and Jaenish, R., Loss of Genomic Methylation Causes p53-Dependent Apoptosis and Epigenetic Deregulation, Nature Genet., 2001, vol. 27, pp. 31–39.
Stancheva, I., Hensey, C., and Meehan, R.R., Loss of the Maintenance Methyltransferase, xDnmt1, Induces Apoptosis in Xenopus Embryos, EMBO J., 2001, vol. 20, pp. 1963–1973.
Vanyushin, B.F., Sevost'yanova, S.S., Kirnos, M.D., and Saidova, N.S., Some Increase in Protein Content Induced by 5-Azacytidine—an Inhibitor of DNA Methylation, Izv. Akad. Nauk SSSR, Ser. Biol., 1990, no. 1, pp. 75–83.
Finnegan, E.J., Genger, R.K., Kovač, K., Peacock, W.J., and Dennis, E.S., DNA Methylation and the Promotion of Flowering by Vernalization, Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 5824–5829.
Aleksandrushkina, N.I., Kirnos, M.D., Rotaenko, E.P., and Vanyushin, B.F., Inhibition of Nuclear DNA Synthesis and Methylation by 5-Azacytidin during Successive Cell Cycles in Dividing Cells of the First Leaf of Etiolated Wheat Seedlings, Biokhimiya, 1989, vol. 54, pp. 355–360.
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Vanyushin, B.F., Shorning, B.Y., Seredina, A.V. et al. The Effects of Phytohormones and 5-Azacytidine on Apoptosis in Etiolated Wheat Seedlings. Russian Journal of Plant Physiology 49, 501–506 (2002). https://doi.org/10.1023/A:1016312009518
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DOI: https://doi.org/10.1023/A:1016312009518