Abstract
Gelatin microspheres with a diameter less than 2 µm were synthesized by means of cross-linking with glutaraldehyde. When the microspheres were subjected to degradation in phosphate-buffered saline solution containing collagenase, the digestion of microspheres was found to decrease with increasing cross-linking. Interferon was incorporated in the microspheres at a high trapping efficiency, and the rate of interferon release from the microspheres was regulated by the extent of cross-linking with glutaraldehyde. Gelatin microspheres incorporating interferon-α were readily phagocytosed by macrophages, regardless of the extent of cross-linking, and the phagocytosed microspheres were observed to be degraded gradually in the interior of macrophages, resulting in the slow release of the incorporated interferon in the cells.
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Tabata, Y., Ikada, Y. Synthesis of Gelatin Microspheres Containing Interferon. Pharm Res 6, 422–427 (1989). https://doi.org/10.1023/A:1015991617704
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DOI: https://doi.org/10.1023/A:1015991617704