Abstract
Etiolated barley (Hordeum vulgare L.) seedlings were treated with heat shock (HS). The heat treatment was conducted daily for 1 h at 40°C over 6 days and led to shortening of leaves and coleoptiles, an increase in the etioplast volume and prothylakoid length, and to a decrease in the size of paracrystalline prolamellar bodies (PLB). As a result of HS treatment, stimulation of carotenoid and protochlorophyllide (Pchlide) synthesis as well as an increase in the relative content of the Pchlide short-wavelength form (Pchlide630) were observed in the leaf tissue of seven-day-old seedlings 12 h after the last HS treatment. HS had no effect on the overall amount of Pchlide-oxidoreductase (POR) in leaves and PLB membranes and did not suppress the Pchlide photoreduction in vivo. PLB membranes, isolated from the HS-treated seedlings, possessed a higher Pchlide and carotenoid content as calculated on total protein basis. These membranes showed more intense protein fluorescence than PLB from untreated plants, whereas hydrophobicity of the microenvironment of the fluorescent amino-acid residues remained unchanged. Studies using pyrene (lipophilic fluorescent probe emitted in Pchlide and carotenoid absorption bands) showed that HS increases the fluidity of membrane lipids in PLB membranes and that the pigments accumulated in these membranes are located in the region of lipid–protein contact site. The results are discussed in relation to the adaptive role of protein–protein and pigment–protein–lipid interactions in etioplast membranes under stress.
Similar content being viewed by others
REFERENCES
Neumann, D., Nover, L., Parthier, B., Rieger, R., Scharf, K.D., Wollgiehn, R., and Zufltieden, U., Heat Shock and Other Stress Response Systems of Plants, Biol. Zentralblat., 1989, vol. 108, pp. 1-156.
Alexandrov, V.Ya. and Kislyuk, I.M., Cell Response to Heat Shock: Physiological Aspect, Tsitologiya, 1994, vol. 36, pp. 5-58.
Veselova, T.V., Veselovskii, V.A., and Chernavskii, D.S., Stress u rastenii (Biofizicheskii podkhod) (Stress in Plants: A Biophysical Approach), Moscow: Mosk. Gos. Univ., 1993.
Leshem, Y.Y. and Kuiper., P.J.C., Is There a GAGS (General Adaptation) Response to Various Types of Environ-mental Stress? Biol. Plant., 1996, vol. 38, pp. 1-18.
Lichtenthaler, H.K., Vegetation Stress: An Introduction to the Stress Concept in Plants, J. Plant Physiol., 1996, vol. 148, pp. 4-14.
Taviadoraci, P., Kloppstech, K., and Argyroudi-Akoyunoglou, J., Circadian Rhythm in the Expression of the mRNA Coding for the Apoprotein of the Light-Harvesting Complex of Photosystem II, Plant Physiol., 1989, vol. 90, pp. 665-672.
Kloppstech, K., Otto, B., and Beator, J., Heat-Induced “Photo” Morphogenesis in Dark-Grown Plants and Circadian Rhythmicity-Is There a Connection? Regulation of Chloroplast Biogenesis. Nato Series, Argyroudi-Akoyunoglou, J., Ed., New York: Plenum, 1991, vol. 226, pp. 91-99.
Argyroudi-Akoyunoglou, J.H., Anastassiou, R., and Bei-Paraskevopoulou, T., Heat-Shock or Red Light-Pulse Induced Circadian Rhythm in the Capacity for Chlorophyll Synthesis in Etiolated Bean or Pea Leaves, Research in Photosynthesis, Murata, N., Ed., Dordrecht: Kluwer, 1992, vol. 3, pp. 327-330.
Kloppstech, K., Otto, B., and Sierralta, W., Cyclic Temperature Treatments of Dark-Grown Pea Seedlings Induce a Rise in Specific Transcript Levels of Light-Regulated Genes to Photomorphogenesis, Mol. Gen. Genet., 1991, vol. 225, pp. 468-473.
Schulz, R. and Senger, H., Protochlorophyllide Reductase: A Key Enzyme in the Greening Process, Pigment-Protein Complexes in Plastids. Synthesis and Assembly, Sundqvist, C. and Ryberg, M., Eds., San Diego: Academic, 1993, pp. 179-218.
Beator, J. and Kloppstech, K., The Effect of Heat Shock on Morphogenesis in Barley: Coordinated Circadian Regulation of mRNA Levels for Light-Regulated Genes and of the Capacity for Accumulation of Chlorophyll-Protein Complexes, Plant Physiol., 1992, vol. 100, pp. 780-786.
Beator, J. and Kloppstech, K., Impact of Circadian Rhytmicity on Development and Gene Expression in Etiolated Barley, Research in Photosynthesis, Murata, N., Ed., Dordrecht: Kluwer, 1992, vol. 3, pp. 331-334.
Sundqvist, C. and Dachlin, C., With Chlorophyll Pigments from Prolamellar Bodies to Light-Harvesting Complexes, Physiol. Plant., 1997, vol. 100, pp. 748-759.
Selstam, E. and Wigge, A.W., Chloroplast Lipids and the Assembly of Membranes, Pigment-Protein Complexes in Plastids: Synthesis and Assembly, Sundqvist, C. and Ryberg, M., Eds., San Diego: Academic, 1993, pp. 241-277.
Ryberg, H., Ryberg, M., and Sundqvist, C., Plastid Ultrastructure and Development, Pigment-Protein Complexes in Plastids: Synthesis and Assembly, Sundqvist, C. and Ryberg, M., Eds., San Diego: Academic, 1993, pp. 25-62.
Kis-Petic, K., Boddi, B., Kaposi, A.D., and Fidy, J., Protochlorophyllide Forms and Energy Transfer in Dark-Grown Wheat Leaves. Studies by Conventional and Laser Excited Fluorescence Spectroscopy between 10 K-100 K, Photosynth. Res., 1999, vol. 60, pp. 87-98.
Dobretsov, G.E., Fluorestsentnye zondy v issledovanii kletok, membran i lipoproteinov (Fluorescent Probes in Study of Cells, Membranes, and Lipoproteins), Moscow: Nauka, 1989.
Vanderkooi, J.M. and Gallis, I.B., Pyrene: A Probe of Lateral Diffusion in the Hydrophobic Region of Membranes, Biochemistry, 1974, vol. 13, pp. 4000-4006.
Radyuk, M.S. and Fradkin, L.I., Effects of High Concentrations of 5-Aminolevulinic Acid on the Plastid Ultra-structure and Spectral Properties of Protochlorophyllide in Barley Leaves, Fiziol. Rast. (Moscow), 1994, vol. 41, pp. 404-408 (Russ. J. Plant Physiol., Engl. Transl.).
Ryberg, M. and Sundqvist, C., Characterization of Prolamellar Bodies and Prothylakoids Fractionated from Wheat Etioplasts, Physiol. Plant., 1982, vol. 56, pp. 125-132.
Hampp, R. and Wellburn, A., Development of Photochemical Activities in Preparations of Unresolved Internal Membranes Enriched with Prolamellar Bodies and Prothylakoid Vesicles during Etioplast-Chloroplast Transformation, Ber. Dtsch. Bot. Ges., 1978, vol. 91, pp. 551-561.
Anderson, J.M. and Boardman, N.K., Studies on the Greening of Dark-Grown Bean Plants: 2. Development of Photochemical Activity, J. Biol. Sci., 1964, vol. 17, pp. 93-99.
Averina, N.G., Sidorko, V.V., and Shalygo, N.V., Spectrofluorimetric Method for Determination of Protoporphyrin IX in Leaves Treated with 5-Aminolevulinic Acid, Vestsi Akad. Navuk BSSR, Ser. Biyal., 1984, no. 4, pp. 102-105.
Shlyk, A.A., Determination of Chlorophylls and Carotenoids in Extracts from Green Leaves, Biokhimicheskie metody v fiziologii rastenii (Biochemical Methods in Plant Physiology), Pavlinova, O.A., Ed., Moscow: Nauka, 1971, pp. 154-170.
Markwell, M.A.K., Hass, S.M., Bicker, L.L., and Tortbert, N.E., A Modification of the Lowry Procedure to Simplify Protein Determination in Membrane and Lipoprotein Samples, Anal. Biochem., 1978, vol. 87, pp. 206-210.
Stocker, J.W., Malavasi, F., and Trucco, M.M., The Determination of Hybridomas Products with Enzyme Immunoassay, Metody immunologii (Methods for Immunology), Moscow: Mir, 1983, pp. 329-338.
Savchenko, G.E., Klyuchareva, E.A., Abramchik, L.M., Serdyuchenko, E.V., Minkov, I.N., and Chaika, M.T., Protochlorophyllide Oxidoreductase and Chlorophyll Biosynthesis in Greening Barley Seedlings, Fiziol. Rast. (Moscow), 1995, vol. 42, pp. 559-566 (Russ. J. Plant Physiol., Engl. Transl.).
Rokitskii, P.F., Biologicheskaya statistika (Biological Statistics), Minsk: Vysheishaya Shkola, 1967.
Sharkova, V.E., Bubolo, L.S., and Kamentseva, I.E., Cell Thermotolerance and the Formation of Heat-Shock Granules in Wheat Leaves, Fiziol. Rast. (Moscow), 1996, vol. 43, pp. 87-93 (Russ. J. Plant Physiol., Engl. Transl.).
Joyard, J., Block, M.A., and Douce, R., Molecular Aspects of Plastid Envelope Biochemistry, J. Biochem., 1991, vol. 199, pp. 489-509.
French, C.S., Fluorescence Spectrophotometry of Photosynthetic Pigments, The Luminiscence of Biological Systems. Proc. Conf. on Luminiscence, Johnson, F.M., Ed., Washington (DC): Am. Assoc. Advanc. Sci., 1955.
Chernitskii, E.A., Lyuminestsentsiya i strukturnaya labil'nost' belkov v rastvore i kletke (Luminescence and Structure Lability of Proteins in the Solution and in the Cell), Minsk: Nauka i Tekhnika, 1972.
Savchenko, G.E., Klyuchareva, E.A., Denev, I.I., and Stupak, A.P., Study on the Pyrene Fluorescence in Galactolipid Liposomes and in the Native Membranes of Etioplasts, Zh. Prikl. Spektroskop., 2000, vol. 67, pp. 342-349.
Young, A.J. and Frank, H.A., Energy Transfer Reactions Involving Carotenoids: Quenching of Chlorophyll Fluorescence, Photochem. Photobiol., 1996, vol. 30, pp. 3-15.
Stress of Life. Stress and Adaptation from Molecules to Man, Abstracts Int. Congr. Stress (1–5 July 1997), Budapest, Hungary, 1997.
Rebeiz, C.A., Montazer-Zouhoor, A., Hopen, H.J., and Wu, S.M., Photodynamic Herbicides: 1. Concept and Phenomenology, Enzyme Microbiol. Technol., 1984, vol. 6, pp. 390-401.
Susek, R.E. and Chory, J., A Tale of Two Genomes: Role of a Chloroplast Signal in Coordinating Nuclear and Plastid Genome Expression, Aust. J. Plant Physiol., 1992, vol. 19, pp. 387-399.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Savchenko, G.E., Klyuchareva, E.A., Abramchik, L.M. et al. Effect of Periodic Heat Shock on the Inner Membrane System of Etioplasts. Russian Journal of Plant Physiology 49, 349–359 (2002). https://doi.org/10.1023/A:1015592902659
Issue Date:
DOI: https://doi.org/10.1023/A:1015592902659