Abstract
Protein stability in microarrays was improved using protein stabilizers. PEG 200 at 30% (w/v) was the most efficient stabilizer giving over 4-fold improvement in protein stability compared to without the stabilizer. PEG 200 above 10% (w/v) in the array solution prevented the evaporation of water in the sample and thereby improved protein stability in the microarray. When the streptavidin-biotin binding reaction was performed under optimized conditions, biotin-BSA-fluorescein isothiocyanate (FITC) was detected from 1 ng ml−1 to 5 μg ml−1 by fluorescence analysis.
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Lee, CS., Kim, BG. Improvement of protein stability in protein microarrays. Biotechnology Letters 24, 839–844 (2002). https://doi.org/10.1023/A:1015554705979
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DOI: https://doi.org/10.1023/A:1015554705979