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Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley

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Abstract

Responses to drought and salinity in barley (Hordeum vulgare L. cv. Tokak) were monitored by microarray hybridization of 1463 DNA elements derived from cDNA libraries of 6 and 10 h drought-stressed plants. Functional identities indicated that many cDNAs in these libraries were associated with drought stress. About 38% of the transcripts were novel and functionally unknown. Hybridization experiments were analyzed for drought- and salinity-regulated sequences, with significant changes defined as a deviation from the control exceeding 2.5-fold. Responses of transcripts showed stress-dependent expression patterns and time courses. Nearly 15% of all transcripts were either up- or down-regulated under drought stress, while NaCl led to a change in 5% of the transcripts (24 h, 150 mM NaCl). Transcripts that showed significant up-regulation under drought stress are exemplified by jasmonate-responsive, metallothionein-like, late-embryogenesis-abundant (LEA) and ABA-responsive proteins. Most drastic down-regulation in a category was observed for photosynthesis-related functions. Up-regulation under both drought and salt stress was restricted to ESTs for metallothionein-like and LEA proteins, while increases in ubiquitin-related transcripts characterized salt stress. A number of functionally unknown transcripts from cDNA libraries of drought-stressed plants showed up-regulation by drought but down-regulation by salt stress, documenting how precisely transcript profiles report different growth conditions and environments.

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  1. Michael Deyholos, Christine B. Michalowski, David W. Galbraith & Hans J. Bohnert

    Present address: Departments of Plant Biology and of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL, 61801, USA

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, University of Arizona, Biosciences West, Tucson, AZ, 85721-0088, USA

    Z. Neslihan Ozturk, Valentina Talamé, Christine B. Michalowski & Hans J. Bohnert

  2. TUBITAK, Marmara Research Center, Research Institute for Genetic Engineering, Turkey

    Z. Neslihan Ozturk & Nermin Gozukirmizi

  3. Department of Agroenvironmental Science and Technology, University of Bologna, 40126, Bologna, Italy

    Valentina Talamé & Roberto Tuberosa

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Ozturk, Z.N., Talamé, V., Deyholos, M. et al. Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley. Plant Mol Biol 48, 551–573 (2002). https://doi.org/10.1023/A:1014875215580

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