Skip to main content
SpringerLink
Log in

A new bio-formulation containing plant growth promoting rhizobacterial mixture for the management of sheath blight and enhanced grain yield in rice

  • Published:
BioControl Aims and scope Submit manuscript

Abstract

Three plant growth promoting rhizobacterial (PGPR) strains, PF1,FP7 and PB2, were tested alone and in combinations for suppression ofrice sheath blight disease and promotion of plant growth underglasshouse and field conditions. The mixture of PGPR strainssignificantly reduced the sheath blight incidence when applied as eitherbacterial suspension through seed, root, foliar and soil application inglasshouse conditions, or as talc-based formulation under fieldconditions, compared to the respective individual strains. The averagemean of disease reduction was 29.2% for single strains and45.1% for mixtures. In addition to disease suppression, treatmentwith mixture of PGPR strains promoted plant growth in terms of increasedplant height and number of tillers, and ultimately grain yield. Theaverage increases in yield for single strains were 17.7%, and25.9% in case of mixture. Mixture of three PGPR strains reduceddisease and promoted growth to a level equivalent to two strainmixtures. Though seed treatment of either single strain or strainmixtures alone could reduce the disease, subsequent application to root,leaves or soil further reduced the disease and enhanced the plantgrowth. The mixture consisting of PF1 plus FP7 was the most effective inreducing the disease and in promoting plant growth and grainyield.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Budge, S.P., M.P. McQuilken, J.S. Fenlon and J.M. Whipps, 1995. Use of Coniothyrium minitans and Gliocladium virens for biological control of Sclerotinia sclerotiorum in glasshouse lettuce. Biol. Control 5: 513-522.

    Google Scholar 

  • Chatterjee, A., V. Valasubramanian, A.K. Ma, W.L. Vachhani, S.S. Gnanamanickam and A.K. Chatterjee, 1996. Isolation of ant mutants of Pseudomonas fluorescens strain Pf 7-14 altered in antibiotic production, cloning of ant+ DNA and evaluation of the role of antibiotic production in the control of blast and sheath blight of rice. Biol. Control 7: 185-195.

    Google Scholar 

  • Cook, R.J., D.M. Weller and E.N. Bassett, 1988. Effect of bacterial seed treatments on growth of recropped wheat in western Washington. Biol. Cult. Tests Control Plant Dis. 3: 53.

    Google Scholar 

  • Dalisay, R.F. and J.A. Kuc, 1995. Persistence of reduced penetration by Colletotrichum lagenarium into cucumber leaves with induced systemic resistance and its relation to enhanced peroxidase and chitinase activities. Physiol. Mol. Plant Pathol. 47: 329-338.

    Google Scholar 

  • Datnoff, L.E., S. Nemec and K. Pernezny, 1995. Biological control of Fusarium crown and root rot of tomato in Florida using Trichoderma harzianum and Glomus intraradices. Biol. Control 5: 427-431.

    Google Scholar 

  • De Meyer, G. and M. Hofte, 1997. Salicylic acid production by the rhizobacterium Pseudomonas aeruginosa 7NSK2 induces resistance to leaf infection by Botrytis cinerea on bean. Phytopathology 87: 588-593.

    Google Scholar 

  • Dowling, D.N. and F. O'Gara, 1994. Metabolites of Pseudomonas involved in the biocontrol of plant disease. Trends Biotechnol. 12: 133-141.

    Google Scholar 

  • Dubeikovsky, A.N., E.A. Mordukhova, V.V. Kochethov, F.Y. Polikarpova and A.M. Boronin, 1993. Growth promotion of black current soft wood cuttings by recombinant strain Pseudomonas fluorescens BSP53a synthesizing an increased amount of indole-3-acetic acid. Soil Biol. Biochem. 25: 1277-1281.

    Google Scholar 

  • Duffy, B.K. and D.M. Weller, 1995. Use of Gaeumannomyces graminis var. graminis alone and in combination with fluorescent Pseudomonas spp. to suppress take-all of wheat. Plant Dis. 79: 907-911.

    Google Scholar 

  • Duffy, B.K., A. Simon and D.M. Weller, 1996. Combination of Trichoderma koningii with fluorescent pseudomonads for control of take-all on wheat. Phytopathology 86: 188-194.

    Google Scholar 

  • Dunne, C., Y. Moenne-Loccoz, J. McCarthy, P. Higgins, J. Powell, D.N. Dowling and F. O'Gara, 1998. Combining proteolytic and phloroglucinol producing bacteria for improved biocontrol of Pythium-mediated damping-off of sugar beet. Plant Pathol. 47: 299-307.

    Google Scholar 

  • Gomez, K.A. and A.A. Gomez, 1984. Statistical Procedure for Agricultural Research. John Wiley and Sons, New York.

    Google Scholar 

  • Janisiewicz, W.J., 1988. Biocontrol of postharvest diseases of apples with antagonist mixtures. Phytopathology 78: 194-198.

    Google Scholar 

  • Janisiewicz, W.J. and B. Bors, 1995. Development of microbial community of bacterial and yeast antagonists to control wound-invading postharvest pathogens of fruits. Appl. Environ. Microbiol. 61: 3261-3267.

    Google Scholar 

  • Janisiewicz, W.J., 1996. Ecological diversity, niche overlap, and coexistence of antagonists used in developing mixtures for biocontrol of postharvest diseases of apples. Phytopathology 86: 473-479.

    Google Scholar 

  • Johnson, K.B., 1994. Dose-response relationships and inundative biological control. Phytopathology 84: 780-784.

    Google Scholar 

  • King, E.O., M.K. Ward and D.E. Raney, 1954. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab Clin. Med. 4: 301-307.

    Google Scholar 

  • Leeman, M., F.M. den Ouden, J.A. van Pelt, C. Cornellissen, A. Matamala-Garros, P.A.H.M. Bakker and B. Schippers, 1996. Suppression of Fusarium wilt of radish by co-inoculation of fluorescent Pseudomonas spp. and root-colonizing fungi. Eur. J. Plant Pathol. 102: 21-31.

    Google Scholar 

  • Leibinger, W., B. Beuker, M. Hahn and K. Mendgen, 1997. Control of postharvest pathogens and colonization of the apple surface by antagonistic microorganisms in the field. Phytopathology 87: 1103-1110.

    Google Scholar 

  • Lifshitz, R., J.W. Kloepper, M. Kozlowski, C. Simonson, J. Cavison, E.M. Tipping and I.I. Zaleska, 1987. Growth promotion of canola (rapeseed) seedlings by a strain of Pseudomonas putida under gnotobiotic conditions. Can. J. Microbiol. 33: 390-395.

    Google Scholar 

  • Mew, T.W. and A.M. Rosales, 1986. Bacterization of rice plants for control of sheath blight caused by Rhizoctonia solani. Phytopathology 76: 1260-1264.

    Google Scholar 

  • M'Piga, P., R.R. Belanger, T.C. Paulitz and N. Benhamou, 1997. Increased resistance to Fusarium oxysporum f.sp. radicis-lycopersici in tomato plants treated with endophytic bacterium Pseudomonas fluorescens strain 63-28. Physiol. Mol. Plant Pathol. 50: 301-320.

    Google Scholar 

  • Nandakumar, R., S. Babu, R. Viswanathan, T. Raguchander and R. Samiyappan, 2000. Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens. Soil Biol. Biochem. (accepted for publication).

  • O'sullivan, D.J. and F. O'Gara, 1992. Traits of Pseudomonas spp. involved in suppression of plant root pathogens. Microbiol. Rev. 56: 662-676.

    Google Scholar 

  • Paulitz, T.C., J.S. Ahmad and R. Baker, 1990. Integration of Pythium nunn and Trichoderma harzianum isolate T-95 for the biological control of Pythium damping-off of cucumber. Plant and Soil 121: 243-250.

    Google Scholar 

  • Pierson, E.A. and D.M. Weller, 1994. Use of mixtures of fluorescent pseudomonads to suppress take-all and improve the growth of wheat. Phytopathology 84: 940-947.

    Google Scholar 

  • Raaijmakers, J.M., M. Leeman, M.M.P. Van Oorschot, I. Van der Sluis, B. Schippers and P.A.H.M. Bakker, 1995. Dose-response relationships in biological control of Fusarium wilt of radish by Pseudomonas spp. Phytopathology 85: 1075-1081.

    Google Scholar 

  • Rabindran, R. and P. Vidhyasekaran, 1996. Development of a formulation of Pseudomonas fluorescens PfALR2 for management of rice sheath blight. Crop Protect. 15: 715-721.

    Google Scholar 

  • Raupach, G.S. and J.W. Kloepper, 1998. Mixtures of plant growth promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology 88: 1158-1164.

    Google Scholar 

  • Rosales, A.M., L. Thomashow, R.J. Cook and T.W. Mew, 1995. Isolation and identification of antifungal metabolites produced by rice-associated antagonistic Pseudomonas spp. Phytopathology 85: 1028-1032.

    Google Scholar 

  • Schisler, D.A., P.J. Slininger and R.J. Bothast, 1997. Effects of antagonist cell concentration and two-strain mixtures on biological control of Fusarium dry rot of potatoes. Phytopathology 87: 177-183.

    Google Scholar 

  • Samiyappan, R., T. Raguchander, K. Jayashree and R. Nandakumar, 1999. Management of major rice diseases with fluorescent pseudomonads. In: M. Subramanian (ed), Vistas of Rice. Tamil Nadu Rice Research Institiute, Tamil Nadu Agricultural University, Tamil Nadu, India. pp. 111-121.

    Google Scholar 

  • Singh, P.P., Y.C. Shin, C.S. Park and Y.R. Chung, 1999. Biological control of Fusarium wilt of cucumber by chitinolytic bacteria. Phytopathology 89: 92-99.

    Google Scholar 

  • Sriram, S., T. Raguchander, P. Vidhyasekaran, S. Muthukrishnan and R. Samiyappan, 1997. Genetic relatedness with special reference to virulence among the isolates of Rhizoctonia solani causing sheath blight in rice. J. Plant Dis. Protect. 104: 260-271.

    Google Scholar 

  • Van Peer, R. and B. Schippers, 1992. Lipopolysaccharides of plant growth-promoting Pseudomonas sp. strain WCS417r induce resistance in carnation to Fusarium wilt. Neth. J. Plant Pathol. 98: 129-139.

    Google Scholar 

  • Vasanthadevi, T., R. Malarvizhi, N. Sakthivel and S.S. Gnanamanickam, 1989. Biological control of sheath blight of rice in India with antagonistic bacteria. Plant and Soil 119: 325-330.

    Google Scholar 

  • Vidhyasekaran, P. and M. Muthamilan, 1995. Development of a formulation of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis. 79: 782-786.

    Google Scholar 

  • Vidhyasekaran, P., R. Rabindran, M. Muthamilan, K. Nayar, K. Rajappan, N. Subramanian and K. Vasumathi, 1997. Development of powder formulation of Pseudomonas fluorescens for control of rice blast. Plant Pathol. 46: 291-297.

    Google Scholar 

  • Vidhyasekaran, P. and M. Muthamilan, 1999. Evaluation of powder formulations of Pseudomonas fluorescens Pf1 for control of rice sheath blight. Biocon. Sci. Technol. 9: 67-74.

    Google Scholar 

  • Wei, G., J.W. Kloepper and S. Tuzan, 1996. Induced systemic resistance to cucumber diseases and increased plant growth by plant growth promoting rhizobacteria under field conditions. Phytopathology 86: 221-224.

    Google Scholar 

  • Weller, D.M. and L.S. Thomashow, 1994. Current challenges in introducing beneficial microorganisms into the rhizosphere. In: O'Gara, D.N. Dowling and B. Boesten (eds), Molecular Ecology of Rhizosphere Microorganisms: Biotechnology and the Release of GMO's. VCH publishers, Weinheim, Germany. pp. 1-18.

    Google Scholar 

  • Zdor, R.E. and A.J. Anderson, 1992. Influence of root colonizing bacteria on the defence responses in bean. Plant and Soil 140: 99-107.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, 641003, India

    R. Nandakumar, S. Babu, T. Raguchander & R. Samiyappan

  2. Sugarcane Breeding Institute, Coimbatore, 641007, India

    R. Viswanathan

  3. Agricultural Research Station, Aliyar Nagar, India

    J. Sheela

Authors
  1. R. Nandakumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Babu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Viswanathan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Sheela
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Raguchander
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. Samiyappan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Samiyappan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nandakumar, R., Babu, S., Viswanathan, R. et al. A new bio-formulation containing plant growth promoting rhizobacterial mixture for the management of sheath blight and enhanced grain yield in rice. BioControl 46, 493–510 (2001). https://doi.org/10.1023/A:1014131131808

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014131131808

Search

Navigation