Abstract
Activation of soluble acid invertase by cytokinin was shown using as a model a detached sugar-beet leaf, one half (sink) of which was treated with benzyladenine and the other half (source) of which was sprayed with water. Acid invertase was assumed to mediate the hormone-induced sink properties of the cells. The influx of 14C-sucrose to the leaf half treated with benzyladenine was induced to much greater extent than that of potassium (86Rb). This suggests different pathways or mechanisms of translocation of these substances to the induced sink.
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REFERENCES
Kursanov, A.L., Prasolova, M.F., and Pavlinova, O.A., The Pathways of Enzymatic Sucrose Transformation in the Sugar Beet Root as Related to Its Sink Function, Fiziol. Rast. (Moscow), 1989, vol. 36, pp. 629-641 (Sov. Plant Physiol., Engl. Transl.).
Mothes, K., Engelbrecht, L., and Kulajewa, O., Ñber die Wirkung des Kinetins auf Stickstoffverteilung und Eiweißynthese in isolierten Blättern, Flora, Ser. A, 1959, vol. 147, pp. 445-464.
Mothes, K. and Engelbrecht, L., On the Activity of a Kinetin-Like Root Factor, Life Sci., 1963, vol. 11, pp. 852-857.
Sokolova, S.V. and Balakshina, N.O., Phytohormone Effect on the Transport and Distribution of 14C-Sucrose in Detached Leaves of Sugar Beet, Fiziol. Rast. (Moscow), 1992, vol. 39, pp. 1088-1097 (Sov. Plant Physiol., Engl. Transl.).
Sokolova, S.V., Phytohormone Involving in the Regulation of Transport and Distribution of Substances in Plants, Peredvizhenie assimilyatov v rasteniyakh i problema sakharonakopleniya (Assimilate Transport in Plants as Related to Sugar Accumulation), Kursanov, A.L. and Pechenov, V.A., Eds., Frunze: Ilim, 1986, pp. 233-256.
Ron'zhina, E.S., Sokolova, S.V., and Mokronosov, A.T., Action of Cytokinins on Transport and Partitioning of Substances in Detached Leaves: 2. Conditions for Developing the Cytokinin Effects, Fiziol. Rast. (Moscow), 1995, vol. 42, pp. 61-67 (Russ. J. Plant Physiol., Engl. Transl.).
Roitsch, T. and Tanner, W., Cell Wall Invertase: Bridging the Gap, Bot. Acta, 1996, vol. 109, pp. 90-93.
Tymowska-Lalanne, Z. and Kreis, M., The Plant Invertases: Physiology, Biochemistry and Molecular Biology, Adv. Bot. Res., 1998, vol. 28, pp. 71-117.
Ehneß, R. and Roitsch, T., Coordinated Induction of mRNAs for Extracellular Invertase and a Glucose Transporter in Chenopodium rubrum by Cytokinins, Plant J., 1997, vol. 11, pp. 539-548.
Pavlinova, O.A. and Prasolova, M.F., Physiological Role of Sucrose Synthase in Sugar Beet Roots, Fiziol. Rast. (Moscow), 1972, vol. 19, pp. 920-927 (Sov. Plant Physiol., Engl. Transl.).
Turkina, M.V. and Sokolova, S.V., The Methods for Determination of Mono-and Oligosaccharides, Biokhimicheskie metody v fiziologii rastenii (Biochemical Methods in Plant Physiology), Pavlinova, O.A., Ed., Moscow: Nauka, 1971, pp. 7-34.
Lowry, O.H., Rosebrough, N.I., Farr, A.L., and Randall, R.J., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, pp. 265-271.
Giaquinta, R., Source and Sink Metabolism in Relation to Phloem Translocation, Plant Physiol., 1978, vol. 61, pp. 380-385.
Morris, D.A. and Arthur, E.D., An Association between Acid Invertase Activity and Cell Growth during Leaf Expansion in Phaseolus vulgaris L., J. Exp. Bot., 1984, vol. 35, pp. 1369-1379.
Pollock, C.J. and Lloyd, E.J., The Distribution of Acid Invertase in Developing Leaves of Lolium temulentum L., Planta, 1977, vol. 133, pp. 197-200.
Kingston-Smith, A.H., Walker, R.P., and Pollock, C.J., Invertase in Leaves: Conundrum or Control Point?, J. Exp. Bot., 1999, vol. 50, pp. 735-743.
Turgeon, R., The Sink-Source Transition in Leaves, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1989, vol. 40, pp. 119-139.
Avigad, G., Sucrose and Disaccharides, Encycl. Plant Physiol., New Ser., Lowns, F.A., Tanner W., Eds., Berlin: Springer-Verlag, 1982, vol. 13A, pp. 217-347.
Sturm, A., Molecular Characterization and Functional Analysis of Sucrose-Cleaving Enzymes in Carrot (Daucus carota L.), J. Exp. Bot., 1996, vol. 47, pp. 1187-1192.
Sturm, A., Sebkova, V., Lorenz, K., Hardegger, M., Lienhard, S., and Unger, C., Developmental-and Organ-Specific Expression of the Genes for Sucrose Synthase and Three Isoenzymes of Acid b-Fructofuranosidase in Carrot, Planta, 1995, vol. 195, pp. 601-610.
Carlson, S.J. and Chowrey, P.S., A Reevaluation of the Relative Roles of Two Invertases, INCW2 and IVR1, in Developing Maize Kernels and Other Tissues, Plant Physiol., 1999, vol. 121, pp. 1025-1035.
Chin, C.K. and Weston, G.D., Distribution in Excised Lycopersicon esculentum Roots of the Principal Enzymes Involved in Sucrose Metabolism, Phytochemistry, 1973, vol. 12, pp. 1229-1235.
Roitsch, T., Bittner, M., and Gadt, D.E., Induction of Apoplastic Invertase of Chenopodium rubrum by D-Glucose and a Glucose Analog and Tissue-Specific Expression Suggest a Role in Sink-Source Regulation, Plant Physiol., 1995, vol. 108, pp. 285-294.
Von Schaewen, A., Stitt, M., Schmidt, R., Sonnewald, U., and Willmitzer, L., Expression of a Yeast-Derived Invertase in the Cell Wall of Tobacco and Arabidopsis Plants Leads to Accumulation of Carbohydrate and Inhibition of Photosynthesis and Strongly Influences Growth and Phenotype of Transgenic Tobacco Plants, EMBO J., 1990, vol. 9, pp. 3033-3044.
Dickinson, C.D., Altabella, T., and Chrispeels, M.J., Slow-Growth Phenotype of Transgenic Tomato Expressing Apoplastic Invertase, Plant Physiol., 1991, vol. 95, pp. 420-425.
Genkov, T., Tsoneva, P., and Ivanova, I., Effect of Cytokinins on Photosynthetic Pigments and Chlorophyllase Activity in in vitro Cultures of Axillary Buds of Dianthus caryophyllus L., J. Plant Growth Regul., 1997, vol. 16, pp. 169-172.
Ron'zhina, E.S., Mokronosov, A.T., and Sokolova, S.V., The Nature of Sink and Retention Effects of Cytokinins in Detached Leaves, Dokl. Akad. Nauk, 1994, vol. 336, pp. 283-286.
Blechschmidt-Schneider, S. and Eschrich, W., Microautoradiographic Localization of Imported 14C-Photosynthate in Induced Sink Leaves of Two Dicotyledonous C4 Plants in Relation to Phloem Unloading, Planta, 1985, vol. 163, pp. 439-447.
Gamalei, Yu.V., Floema lista (Leaf Phloem), Leningrad: Nauka, 1990.
Hayes, P.M. and Patrick, J.W., Photosynthate Transport in Stem of Phaseolus vulgaris L. Treated with Gibberellic Acid, Indole-3-Acetic Acid or Kinetin, Planta, 1985, vol. 166, pp. 371-379.
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Sokolova, S.V., Balakshina, N.O. & Krasavina, M.S. Activation of Soluble Acid Invertase Accompanies the Cytokinin-Induced Source–Sink Leaf Transition. Russian Journal of Plant Physiology 49, 86–91 (2002). https://doi.org/10.1023/A:1013768429467
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DOI: https://doi.org/10.1023/A:1013768429467