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Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli

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Abstract

A second promoter (P1) was inserted to the PHA (poly-β-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5α with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-β-hydroxybutyrate (PHB) with long chain length. When the inducer, IPTG was added to the culture at 0.4 mM, the average molecular weight was 1.1 × 106 Da. However, an even greater increase of the PHB average molecular weight to 2.5 × 107 Da was observed without IPTG being added.

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Authors and Affiliations

  1. Water Environment and Remediation Research Center, Korea Institute of Science and Technology, Seoul, 136-791, Korea

    Sang Jun Sim

  2. Metabolix, Inc., MA, 02142, USA

    Kristi D. Snell

  3. Department of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Korea

    Byung Woo Kim

  4. Biomaterials Science and Engineering Laboratory, USA

    Cho Kyun Rha

  5. Department of Biology, Massachusetts Institute of Technology, MA, 02139, USA

    Anthony J. Sinskey

Authors
  1. Sang Jun Sim
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  2. Kristi D. Snell
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  3. Byung Woo Kim
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  4. Cho Kyun Rha
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  5. Anthony J. Sinskey
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Sim, S.J., Snell, K.D., Kim, B.W. et al. Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli . Biotechnology Letters 23, 2057–2061 (2001). https://doi.org/10.1023/A:1013752000022

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  • DOI: https://doi.org/10.1023/A:1013752000022

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