Skip to main content
SpringerLink
Log in

Sucrose-Phosphate Synthase, Sucrose Synthase, and Invertase in Sugar Beet Leaves

  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

The activity of sucrose-phosphate synthase (SPS) in sugar beet (Beta vulgaris L.) leaves was shown to exceed considerably the synthesizing activity of sucrose synthase (SS). The rise in SPS activity was related to the daylight period; i.e., it was associated with the rate of photosynthesis. The highest SPS activity was characteristic of fully expanded source leaves. In young developing leaves (leaves expanded to less than half of their final size), which represent the sink organs, the SPS activity was 2.5 times lower. At all stages of leaf development, the synthesizing SS activity was rather low. The diurnal change of SS activity was independent of photosynthesis and showed a slight rise from 6:00–8:00 p.m. Under field conditions, the highest SPS activity was found in leaves in the terminal stage of their development (105-day-old plants); the synthesizing activity of SS showed little changes during this period. The activity of soluble acid invertase was characteristic of young leaves. In mature leaves, the activity of this enzyme correlated with the daylight period. These changes occurred on the background of low sucrose content in leaves. The regulation of SPS, SS, and invertase activity is discussed. It is supposed that compartmentation of these enzymes in the photosynthesizing cell is important for transport, metabolism, and the osmotic function of sucrose in leaves.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Kursanov, A.L., Transport assimilyatov v rasteniti, Moscow: Nauka, 1976. Translated under the title Assimilate Transport in Plants, Amsterdam: Elsevier, 1984.

    Google Scholar 

  2. Kursanov, A.L., Prasolova, M.F., and Pavlinova, O.A., The Pathways of Enzymatic Sucrose Transformation in the Sugar Beet Root as Related to Its Sink Function, Fiziol. Rast. (Moscow), 1989, vol. 36, pp. 629-641 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  3. Pavlinova, O.A. and Prasolova, M.F., Physiological Role of Sucrose Synthase in the Sugar Beet Roots, Fiziol. Rast. (Moscow), 1972, vol. 19, pp. 920-925 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  4. Huber, S.C., Nielsen, T.H., Huber, J.L.A., and Pharr, D.M., Variation among Species in Light Activation of Sucrose-Phosphate Synthase, Plant Cell Physiol., 1989, vol. 30, pp. 277-285.

    Google Scholar 

  5. Pavlinova, O.A., Prasolova, M.F., and Balakhontsev, E.N., Study on the Enzymes of Sucrose Synthesis and Cleavage in Sugar Beet Leaves, Fiziologicheskie i tekhnologicheskie aspekty povysheniya produktivnosti sel'skokhozyaistvennykh kul'tur (Physiological and Technological Aspects of Improved Productivity of Crop Plants), Ufa: Bashkir. Nauchn. Tsentr, Ural. Otd. Ross. Akad. Nauk, 1992, pp. 3-9.

    Google Scholar 

  6. Kursanov, A.L. and Pavlinova, O.A., About Site of Sucrose Synthesis in Sugar Beet Plant, Biokhimiya, 1952, vol. 17, pp. 446-455.

    Google Scholar 

  7. Pavlinova, O.A., Application of Isotope Method to the Study of Sucrose Synthesis in the Sugar Beet Plant, Biokhimiya, 1954, vol. 19, pp. 364-372.

    Google Scholar 

  8. Kursanov, A.L. and Turkina, M.V., About Transport Forms of Sugars in the Conductive System of Sugar Beet Plant, Dokl. Akad. Nauk SSSR, 1954, vol. 95, pp. 885-888.

    Google Scholar 

  9. Turkina, M.V., The Synthesis and Transport of Sucrose in the Sugar Beet Plant, Biokhimiya, 1954, vol. 19, pp. 357-363.

    Google Scholar 

  10. Cardini, C.E., Leloir, L.F., and Chiriboga, J., The Biosynthesis of Sucrose, J. Biol. Chem., 1955, vol. 214, pp. 149-155.

    Google Scholar 

  11. Leloir, L.F. and Cardini, C.E., The Biosynthesis of Sucrose Phosphate, J. Biol. Chem., 1955, vol. 214, pp. 157-165.

    Google Scholar 

  12. Heber, U. and Walker, D.A., The Chloroplast Envelope-Barrier or Bridge? Trends Biochem. Sci., 1979, vol. 4, pp. 252-256.

    Google Scholar 

  13. Fry, S.C. and Bidwell, G.S., An Investigation of Photosynthetic Sucrose Production in Bean Leaves, Can. J. Bot., 1977, vol. 55, pp. 1457-1464.

    Google Scholar 

  14. Huber, S.C. and Huber, J.L., Role of Sucrose-Phosphate Synthase in Sucrose Metabolism in Leaves, Plant Physiol., 1992, vol. 99, pp. 1275-1278.

    Google Scholar 

  15. Weiner, H., McMichael, R.W., Jr., and Huber, S.C., Identification of Factors Regulating the Phosphorylation Status of Sucrose-Phosphate Synthase in vivo, Plant Physiol., 1992, vol. 99, pp. 1435-1442.

    Google Scholar 

  16. Worrell, A.C., Bruneau, J.-M., Summerfelt, K., Boersig, M., and Voelker, T.A., Expression of a Maize Sucrose-Phosphate Synthase in Tomato Alters Leaf Carbohydrate Partitioning, Plant Cell, 1991, vol. 3, pp. 1121-1130.

    Google Scholar 

  17. Nguyen-Quoc, B., N'Tchobo, H., and Foyer, C.H., Over-expression of Sucrose-Phosphate Synthase Increases Sucrose Unloading in Transformed Tomato Fruit, J. Exp. Bot., 1999, vol. 50, pp. 785-791.

    Google Scholar 

  18. D'Aoust, M.-A., Yelle, S., Nguyen-Quoc, B., Antisense Inhibition of Tomato Fruit Sucrose Synthase Decreases Fruit Setting and Sucrose Unloading Capacity of Young Fruit, Plant Cell, 1999, vol. 11, pp. 2407-2418.

    Google Scholar 

  19. Signora, L., Galtier, N., Skot, L., Lucas, H., and Foyer, C.H., Overexpression of Sucrose Phosphate Synthase in Arabidopsis thaliana Results in Increased Foliar Sucrose/Starch Ratios and Favours Decreased Foliar Carbohydrate Accumulation in Plants after Prolonged Growth with CO2 Enrichment, J. Exp. Bot., 1998, vol. 49, pp. 669-680.

    Google Scholar 

  20. Hesse, H., Sonnewald, U., and Willmitzer, L., Cloning and Expression Analysis of Sucrose-Phosphate Synthase from Sugar Beet (Beta vulgaris L.), Mol. Gen. Genet., 1995, vol. 247, pp. 515-520.

    Google Scholar 

  21. Choiu, T.J. and Bursh, D.R., Sucrose Is a Signal Molecule in Assimilate Partitioning, Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 4784-4788.

    Google Scholar 

  22. Pavlinova, O.A. and Turkina, M.V., Biosynthesis and Physiological Role of Sucrose in Plants, Fiziol. Rast. (Moscow), 1978, vol. 25, pp. 1025-1041 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  23. Dubinina, I.M., Kudryavtseva, L.F., and Burakhanova, E.A., Product Compartmentation in the Vacuoles of Protoplasts Isolated from Beta vulgaris L. Mesophyll, Fiziol. Rast. (Moscow), 1992, vol. 39, pp. 1098-1107 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  24. Heineke, D., Wildenberger, K., Sonnewald, U., Willmitzer, L., and Heldt, H.W., Accumulation of Hexoses in Leaf Vacuoles: Studies with Transgenic Tobacco Plants Expressing Yeast-Derived Invertase in the Cytosol, Vacuole or Apoplast, Planta, 1994, vol. 194, pp. 29-33.

    Google Scholar 

  25. Kingston-Smith, A.H., Walker, R.P., and Pollock, C.J., Invertase in Leaves: Conundrum or Control Point? J. Exp. Bot., 1999, vol. 50, pp. 735-743.

    Google Scholar 

  26. Poolman, M.G., Fell, D.A., and Thomas, S., Modelling Photosynthesis and Control, J. Exp. Bot., 2000, vol. 51, pp. 319-328.

    Google Scholar 

  27. Lunn, J.E., Price, G.D., and Furbank, R.T., Cloning and Expression of a Prokaryotic Sucrose-Phosphate Synthase Gene from the Cyanobacterium Synechocystis sp. PCC 6803, Conf. Assimilate Transport and Partitioning. Abstr., Newcastle (Australia), 1999, p. 102.

  28. McLean, B., Whatley, J.M., and Juniper, B.E., Continuity of Chloroplast and Endoplasmic Reticulum Membranes in Chara and Equisetum, New Phytol., 1988, vol. 109, pp. 59-65.

    Google Scholar 

  29. Wenter, H., Huber, J.L., and Huber, S.C., Identification of Sucrose Synthase as an Actin-Binding Protein, FEBS Lett., 1998, vol. 430, pp. 205-208.

    Google Scholar 

  30. Delmer, D.P. and Amor, Y., Cellulose Biosynthesis, Plant Cell, 1995, vol. 7, pp. 987-1000.

    Google Scholar 

  31. Amor, Y., Haigler, C.H., Johnson, S., Wainscott, M., and Delmer, D.P., A Membrane-Associated Form of Sucrose Synthase and Its Potential Role in Synthesis of Cellulose and Callose in Plants, Proc. Natl. Acad. Sci. USA, 1995, vol. 92, pp. 9353-9357.

    Google Scholar 

  32. Kursanov, A.L., Enzyme Working in Living Cell, Fermenty (Enzymes), Moscow: Izd. Akad. Nauk SSSR, 1940, pp. 203-226.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    O. A. Pavlinova, M. F. Prasolova & M. V. Turkina

  2. Bashkir Research Center, Ural Division, Russian Academy of Sciences, Ufa, Bashkortostan, Russia

    E. N. Balakhontsev

Authors
  1. O. A. Pavlinova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. N. Balakhontsev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. F. Prasolova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. V. Turkina
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pavlinova, O.A., Balakhontsev, E.N., Prasolova, M.F. et al. Sucrose-Phosphate Synthase, Sucrose Synthase, and Invertase in Sugar Beet Leaves. Russian Journal of Plant Physiology 49, 68–73 (2002). https://doi.org/10.1023/A:1013712311720

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1013712311720

Search

Navigation