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Heterogeneity of the Nucleotide Sequences of the 16S rRNA Genes of the Type Strain of Desulfotomaculum kuznetsovii

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Abstract

Two copies of the 16S rRNA gene, rrnAand rrnB, of the type strain 17Tof the thermophilic sulfate-reducing bacterium Desulfotomaculum kuznetsoviiwere cloned and completely sequenced. The comparison of the determined sequences revealed considerable heterogeneity (8.3%) of the two genes, rrnAand rrnB.The main differences were associated with superlong inserts located in the variable 5"- and 3"-terminal regions of the 16S rRNA genes. Comparative analysis that involved analogous genes from the phylogenetically closest representatives of the genus Desulfotomaculumshowed that disregard of the heterogeneity of the two gene copies distorts the position of the bacterium studied in the phylogenetic tree.

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Authors and Affiliations

  1. Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    T. P. Tourova, E. V. Novikova & T. N. Nazina

  2. Bioengineering Center, Russian Academy of Sciences, Moscow, Russia

    B. B. Kuznetzov

  3. Engelhardt Institute of Molecular Biology, ul. Vavilova 32, Moscow, GSP-1, 117984, Russia

    A. B. Poltaraus

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  1. T. P. Tourova
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  2. B. B. Kuznetzov
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  3. E. V. Novikova
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  4. A. B. Poltaraus
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  5. T. N. Nazina
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Correspondence to T. P. Tourova.

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Tourova, T.P., Kuznetzov, B.B., Novikova, E.V. et al. Heterogeneity of the Nucleotide Sequences of the 16S rRNA Genes of the Type Strain of Desulfotomaculum kuznetsovii. Microbiology 70, 678–684 (2001). https://doi.org/10.1023/A:1013135831669

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