Skip to main content
Log in

Lead Solubilization and Accumulation by Two Strains of Pseudomonas Obtained from a Contaminated Alfisol's Effluent in Southwestern Nigeria

  • Published:
Environmental Monitoring and Assessment Aims and scope Submit manuscript

Abstract

Two strains of Pseudomonas species (B2 and D5)selected from an array of lead solubilizing and accumulatingbacteria obtained from the effluent contaminated soil samples of abattery manufacturing factory were studied. Increase in pH between 4.0 and 6.0 favoured the growth of isolates: Peaklog10 cfu mL−1 values of 7.1, 7.5 and 8.5 wereobtained at pH 4, 5 and 6, respectively.Cell bound lead concentrations for B2 (0.34 mg mL−1)and D5 (0.30 mg mL−1) obtained by direct contact withPbs were greater than lead concentrations of 0.89 and 0.25 mg mL−1 for B2 and D5, respectively,obtained in dialyzed cultures. These cell bound lead concentration in undialyzed cultures were alsogreater than lead concentrations of 0.03 and 0.07 mg mL−1 for B2 and D5 in culture supernatants. Glucose addition did nor improve lead accumulation in the isolates.Exploitation of such isolates for the biotreatment of lead ladeneffluent was conducted.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  • Alexander, M.: 1977, Introduction to Soil Microbiology, John Wiley & Sons, New York, 235 pp.

    Google Scholar 

  • Arrouays, D., Moench, M., Amans, V. and Gomez, A.: 1995, ‘Short range variability of fallout of lead in contaminated soil’, Can. J. Soil Sci. 76, 73–81.

    Google Scholar 

  • Benka-Coker, M. O.: 1991, ‘Studies of the Effect of Crude Oil and Heavy Metal Contamination of Soil and Water on the Microbial Populations at the Kokori Flare Site, Bendel State, Nigeria, Ph. D. Thesis, University of Benin, 165 pp.

  • Biney, C., Amuzu, A. Y., Calamari, D., Kaba, N., Mbome, I. L., Naeve, H., Ochumba, P. B., Osibanjo, O., Radegonde, V. and Saad, M. A.: 1994, ‘Review of Heavy Metals’, Review of Pollution in the African Aquatic Environment, CIFA Technical Paper 25, 33–51.

    Google Scholar 

  • Blackwell, K. J., Singleton, T. and Tobin, J.: 1995, ‘Metal cation uptake by Yast: A review’, Appl. Microbiol Biotechnol. 43, 579–584.

    Google Scholar 

  • Brady, D. and Duncan, A.: 1994, ‘Bioaccumulation of metal cations by Saccharomyces cerevisiae’, App. Microbiol Biotechnol. 41, 149–159.

    Google Scholar 

  • Buchanan, R. E. and Gibbons, N. E.: 1974, Bergey's Manual of Determinative Bacteriology, 8th edn., Williams and Wilkens Comp., Baltimore, 1268 pp.

    Google Scholar 

  • Chaudry, R., Ashish, P. and Mukerji, K.: 1989, ‘Effect of Some Atmospheric Pollutants on Micro-Organisms’, in K. Mukerji, V. Singh, and K. Garg (eds.), Frontiers in Applied Microbiology, Rastogi and Co., India, pp. 84–89.

    Google Scholar 

  • Christensen, E. R., Scherfig, J. and Dixon, P. S.: 1979, ‘Effects of manganese, copper and lead on Selenastrum capricornutum and Chlorella stigmatophora, Water Res. 13, 79–92.

    Google Scholar 

  • Cole, M.: 1979, ‘Solubilization of heavy metal sulfides by heterotrophic soil bacteria’, Soil Sci. 127, 313–317.

    Google Scholar 

  • Cowan, S. T. and Steel, K. J.: 1994, Manual for the Identification of Medical Bacteria, 2nd edn., Cambridge University Press, 238 pp.

  • Doelman, P. and Haanstra, L.: 1979, ‘Effect of lead on soil respiration and dehydrogenase’, Soil Biol. Biochem 11, 47–480.

    Google Scholar 

  • Francis, A. and Dodge, C.: 1986, ‘Anaerobic bacterial dissolution of lead oxide’, Arch. Environ. Contam. Toxicol. 15, 611–616.

    Google Scholar 

  • Gadd, G. M.: 1990, ‘Metal Tolerance’, in Microbes of Extreme Environments, Open University Press, Britain, 210 pp.

    Google Scholar 

  • Hanson, N. W.: 1973, Official Standardized and Recommended Methods of Analysis, Society for Analytical Chemistry, London, U.K., 495 pp.

    Google Scholar 

  • Huang, C. P., Westman, D., Quirk, K. and Huang, J. P.: 1988, ‘The removal of Cd2+ from dilute aqueous solution of fungal absorbent’, Water Sc. Tech. 20, 369–376.

    Google Scholar 

  • Hughes, M. and Poole, R.: 1989, Metals and Micro-Organisms, Chapman and Hall, U.K., 396 pp.

    Google Scholar 

  • Margesin, R. and Schinner, F.: 1996, ‘Baterial Heavy Metal Tolerance – Extreme Resistance to Nickel in Arthrobacter sp.’, J. Basic Microbiol. 36, 269–282.

    Google Scholar 

  • Saguir, M. F. and Nadia de Nanca, M. C.: 1996, ‘Organic acid metabolism under different glucose concentration of Leuconostoc oenes from Wine’, J. Appl. Bact. 81, 394–399.

    Google Scholar 

  • Summers, A. O. and Silver, S.: 1878, ‘Microbial transportation of metals’, Ann. Rev. Microbiol. 32, 637–672.

    Google Scholar 

  • Tornabene, T. and Edwards, H.: 1972, ‘Microbial uptake of lead’, Science 176, 1334–1335.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ekundayo, E.O., Killham, K. Lead Solubilization and Accumulation by Two Strains of Pseudomonas Obtained from a Contaminated Alfisol's Effluent in Southwestern Nigeria. Environ Monit Assess 71, 243–254 (2001). https://doi.org/10.1023/A:1011827716290

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1011827716290

Navigation