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Development of Systems for Targeting the Regional Lymph Nodes for Diagnostic Imaging: In Vivo Behaviour of Colloidal PEG-Coated Magnetite Nanospheres in the Rat Following Interstitial Administration

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Abstract

Purpose. Nanoparticles can be utilised for targeting drugs to the regional lymph nodes or as diagnostic agents. The surface modification of magnetite nanospheres with poly(ethylene glycol) (PEG) has been assessed by in vitro characterisation and in vivo studies following subcutaneous administration to the rat.

Methods. Magnetite nanospheres were prepared with a grafted PEG layer using various PEG lengths from 350 to 1000 Da. Thermogravimetric analysis was utilised to measure the adsorbed amount of PEG. Colloid stability was confirmed by measurement of the particle size and electrophoretic mobility. The kinetics of injection site drainage and lymph node retention were determined 2 hours after subcutaneous administration, for nanospheres coated with PEG lengths of 350, 550, 750, and 1000 Da. For the 750 PEG coated nanospheres, the kinetics of distribution was determined over a 48–hour time course.

Results. The distribution of the nanospheres was modified and the lymph node localisation enhanced by altering the surface coverage of PEG on the magnetic surface.

Conclusions. PEG–coated magnetite nanospheres with different surface characteristics can be utilised to target a diagnostic agent to regional lymph nodes.

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Authors and Affiliations

  1. Institute of Pharmaceutical Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK

    L. Illum

  2. West Pharmaceutical Services, Drug Delivery & Clinical Research Centre Ltd., Albert Einstein Centre, Nottingham Science & Technology Park, Nottingham, NG7 2TN, UK

    L. Illum & A. E. Church

  3. Institute of Pharmaceutical Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK

    M. D. Butterworth, A. Arien, J. Whetstone & S. S. Davis

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  1. L. Illum
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  2. A. E. Church
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  3. M. D. Butterworth
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  4. A. Arien
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  5. J. Whetstone
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  6. S. S. Davis
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Illum, L., Church, A.E., Butterworth, M.D. et al. Development of Systems for Targeting the Regional Lymph Nodes for Diagnostic Imaging: In Vivo Behaviour of Colloidal PEG-Coated Magnetite Nanospheres in the Rat Following Interstitial Administration. Pharm Res 18, 640–645 (2001). https://doi.org/10.1023/A:1011081210142

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