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Evidence for the Utility of the Bm86 Antigen from Boophilus Microplus in Vaccination Against Other Tick Species

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Abstract

The Bm86 antigen, as originally identified in Boophilus microplus, is the basis of commercial tick vaccines against this tick species. The potential for using this antigen or homologues of the antigen in vaccination against other tick species has been assessed. We have conducted vaccine trials in cattle using the B. microplus-derived recombinant Bm86 vaccine (TickGARD™) using pairs of vaccinated calves and control calves. These were infested with B. microplus and Boophilus decoloratus larvae simultaneously. For both species, the numbers of engorged female adult ticks, their weight and egg-laying capacity were all reduced, leading to a reduction in reproductive capacity of 74% for B. microplus and 70% for B. decoloratus. Hyalomma anatolicum anatolicum ticks were fed both as immatures as well as adults on vaccinated calves and non-vaccinated controls. There was an overall 50% reduction in the total weight of nymphs engorging on vaccinated calves, and a suggestion of a subsequent effect on feeding adults. For Hyalomma dromedarii there was a 95% reduction in the number of nymphs engorging and a further 55% reduction in weight of those ticks surviving. Rhipicephalus appendiculatus and Amblyomma variegatum ticks were fed simultaneously both as immatures and subsequently as adults. There was no evidence for a significant vaccination effect. Finally, the amino acid sequence of a Bm86 homologue found in H. a. anatolicum unequivocally demonstrated the conservation of this molecule in this species. Our strategy for the development of multivalent anti-tick vaccines is discussed in relation to these findings.

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References

  • Ambrose, N., Lloyd, D. and Maillard J-C, 1999. Immune responses to Dermatophilus congolensis infections. Parasitol Today 15(7): 295-300.

    Google Scholar 

  • Canales, M., Enríquez, A., Ramos, E., Cabrera, D., Dandie, H., Soto, A., Falcón, V., Rodríguez, M. and de la Fuente, J. 1997. Large-scale production in Pichia pastoris of the recombinant vaccine GavacTM against cattle tick. Vaccine 15: 414-422.

    Google Scholar 

  • Cobon, G.S. 1997 An anti-arthropod vaccine: TickGARD-A vaccine to prevent cattle tick infestations. In: New generation vaccines, M.M. Levine, G.C. Woodrow, J.B. Kaper. and G.S. Cobon, (eds.), Marcel Dekker, Inc., New York, Basel, Hong Kong.

    Google Scholar 

  • Cobon, G.S., Moore, J.T., Johnston, Law A.Y., Willadsen, P., Kemp, D.H., Sriskantha, A., Riding, G.A., Rand, K.N. 1996. DNA encoding a cell membrane glycoprotein of a tick gut. US Patent & Trademark Office Appl. No. 325071 435/240.2.

  • Floyd, R.B., Sutherst, R.W. and Hungerford, J. 1995. Modelling the field efficacy of a genetically engineered vaccine against the cattle tick, Boophilus microplus. Int. J. Parasitol. 25: 285-291.

    Google Scholar 

  • Fragoso, H., Rad, P.H., Ortiz, M., Rodriguez, M., Redondo, M., Herrera, L. and de la Fuente, J. 1998. Protection against Boophilus annulatus infestations in cattle vaccinated with the B. microplus Bm86-containing vaccine GAVAC. Vaccine 16(20): 1990-1992.

    Google Scholar 

  • Garcia-Garcia, J.C., Gonzalez, I.L., Gonzalez, D. M., Valdes, M., Mendez, L., Lamberti, J., D'Agostino, B., Citroni, D., Fragoso, H., Ortiz, M., Rodriguez, M. and de la Fuente, J. 1999. Sequence variations in the Boophilus microplus Bm86 locus and implications for immunoprotection in cattle vaccinated with this antigen. Exp. Appl. Acarol. 23: 883-895.

    Google Scholar 

  • Garcia-Garcia, J.C., Montero, C., Redondo, M., Vargas, M., Canales, M., Boue, O., Rodriguez, M., Joglar, M., Machado, H., Gonzalez, I.L., Valdes, M., Mendez, L. and de la Fuente, J. 2000. Control of ticks resistant to immunization with Bm86 in cattle vaccinated with the recombinant antigen Bm95 isolated from the cattle tick, Boophilus microplus. Vaccine 18(21): 2275-2287.

    Google Scholar 

  • Hamilton, S.E., Kemp, D.H., McKenna, R.V. and Willadsen, P. 1991. Gut cells of the tick Boophilus microplus: the effects of vaccination on digest cells and experiments on blood meal absorption by these cells. In: Modern acarology, F. Dusbabek and V. Bukva (eds.), Vol. 1, pp. 341-351. SPB Academic Publishing, The Hague.

    Google Scholar 

  • Johnston, L.A.Y., Kemp, D.H. and Pearson, R.D. 1986. Immunisation of cattle against Boophilus microplus using extract derived from adult female ticks: effects of induced immunity on tick populations. Int. J. Parasitol. 16: 27-34.

    Google Scholar 

  • Jongejan F. and Uilenberg G. 1994. Ticks and control methods. Rev. Sci. Tech. 13(4): 1201-1226.

    Google Scholar 

  • Khalaf-Allah S.S. 1999. Control of Boophilus microplus ticks in cattle calves by immunization with a recombinant Bm86 glucoprotein antigen preparation. Dtsch Tierarztl Wochenschr. 106(6): 248-251.

    Google Scholar 

  • Kemp, D.H., Pearson, R.D., Gough, J.M. and Willadsen, P. 1989. Vaccination against Boophilus microplus: localization of antigens on tick gut cells and their interaction with the host immune system. Exp. Appl. Acarol. 7(1): 43-58.

    Google Scholar 

  • Labarta, V., Rodríguez, M., Penichet, M., Lleonart, R., Lorenzo Luaces, L. and de la Fuente, J. 1996. Simulation of control strategies for the cattle tick Boophilus microplus employing vaccination with a recombinant Bm86 antigen preparation. Vet. Parasitol. 63: 131-160.

    Google Scholar 

  • Nolan, J. 1990. Acaricide resistance in single and multi-host ticks and strategies for control. Parasitol. 32: 145-153.

    Google Scholar 

  • Pruett, J.H. 1999. Immunological control of arthropod ectoparasites-a review. Int J Parasitol. 29(1): 25-32.

    Google Scholar 

  • Rand, K., Moore, T., Srikatha, A., Spring, K., Tellam, R., Willadsen, P. and Cobon, G.S. 1989. Cloning and expression of a protective antigen from cattle tick Boophilus microplus. Proc. Natl. Acad. Sci. USA 86: 9657-9661.

    Google Scholar 

  • Riding, G.A., Jarmey, J., McKenna, R.V., Pearson, R., Cobon, G.S. and Willadsen, P. 1994. A protective ‘concealed’ antigen from Boophilus microplus: purification, localization and possible function. J. Immunol. 153: 5158-5166.

    Google Scholar 

  • Rodríguez, M., Rubiera, R., Penichet, M., Montesino, R., Cremata, J., Falcon, V., Sanchez, G., Bringas, R., Cordoves, C., Valdez, M., Lleonart, R., Herrera, L and de la Fuente, J. 1994. High level expression of the Boophilus microplus Bm86 antigen in the yeast P. pastoris forming highly immunogenic particle for cattle. J. Biotech. 33: 135-146.

    Google Scholar 

  • Rodríguez, M., Penichet, M., Mouris, A. E., Labarta, V., Lorenzo Luaces, L., Rubiera, R., Cordoves, C., Sanchez, P., Ramos, E., Soto, A., Canales, M., Palenzuela, D., Triguero, A., Lleonart, R., Herrera, L. and de la Fuente, J. 1995. Control of Boophilus microplus populations in grazing cattle vaccinated with a recombinant Bm86 antigen preparation. Vet. Parasitol. 57: 339-349.

    Google Scholar 

  • Rodríguez, M., Massard, C., Henrique da Fonseca, A, Fonseca, R. N., Machado, H., Labarta, V. and de la Fuente, J. 1995. Effect of vaccination with a recombinant Bm86 antigen preparation on natural infestations of Boophilus microplus in grazing dairy and beef pure and cross-bred cattle in Brazil. Vaccine 13: 1804-1808.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. In: Molecular cloning, a laboratory manual, Cold Spring H Laboratory Press, Cold Spring Harbour, NY.

    Google Scholar 

  • Tellam, R.L., Smith, D., Kemp, D.H. and Willadsen, P., 1992. Vaccination against ticks. In: Animal parasite control utilizing biotechnology, W.K. Yong (ed.), pp. 303-331. CRC Press, Boca Raton.

    Google Scholar 

  • Willadsen, P. 1997. Novel vaccine for ectoparasites. Vet. Parasitol. 71: 209-222.

    Google Scholar 

  • Willadsen, P. The molecular revolution in the development of vaccines against ectoparasites. Vet. Parasitol. In press.

  • Willadsen, P. and Kemp, D.H., 1988. Vaccination with concealed antigens for tick control. Parasitol. Today 4: 196-198.

    Google Scholar 

  • Willadsen, P., Riding, G.A., McKenna, R.V., Kemp, D.H., Tellam, R.L., Nielsen, J.N., Lahstein, J., Cobon, G.S. and Gough, J.M. 1989. Immunological control of a parasitic arthropod: identification of a protective antigen from Boophilus microplus. J. Immunol. 143: 1346-1351.

    Google Scholar 

  • Willadsen, P., Bird, P., Cobon, G.S. and Hungerford, J. 1995. Commercialisation of a recombinant vaccine against Boophilus microplus. Parasitol. 110: S43-50.

    Google Scholar 

  • Willadsen, P., Smith, D., Cobon, G. and McKenna, R.V. 1996. Comparative vaccination of cattle against Boophilus microplus with recombinant antigen Bm86 alone or in combination with recombinant Bm91. Parasite Immunol. 18: 241-246.

    Google Scholar 

  • Willadsen, P. and Jongejan, F. 1999. Immunology of the tick-host interaction and the control of ticks and tick-borne diseases. Parasitol Today 15(7): 258-62.

    Google Scholar 

  • Wikel, S.K. 1988. Immunological control of hematophagous arthropod vectors: utilization of novel antigens. Vet. Parasitol. 29: 235-264.

    Google Scholar 

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De Vos, S., Zeinstra, L., Taoufik, A. et al. Evidence for the Utility of the Bm86 Antigen from Boophilus Microplus in Vaccination Against Other Tick Species. Exp Appl Acarol 25, 245–261 (2001). https://doi.org/10.1023/A:1010609007009

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