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Array Biosensor: Optical and Fluidics Systems

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Abstract

Optical and fluidics systems have been developed as central components for an automated array biosensor. Disposable planar waveguides are patterned with immobilized capture antibodies using a physically isolated patterning (PIP) method. The PIP method enables simultaneous deposition of several antibodies and completely circumvents cross-immobilization problems encountered with other array deposition processes. A multi-channel fluidics cell allows numerous assays to be performed on the patterned waveguide. The sensing arrays are optically interrogated using a diode laser with a tailored output to optimize coupling to and maximize excitation uniformity within the waveguide. A patterned cladding is employed to optically isolate the waveguide from perturbations induced by the permanently attached flow cells. Compact optics image the evanescently excited fluorescence onto a large area, cooled CCD array. The image data is processed and automated signal analysis corrects for local background and noise variations.

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Authors and Affiliations

  1. Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, D.C, 20375-5348, USA

    Mark J. Feldstein, Joel P. Golden, Chris A. Rowe & Frances S. Ligler

  2. Physics Department, Dublin City University, Glasnevin, Dublin 9, Ireland

    Brian D. MacCraith

Authors
  1. Mark J. Feldstein
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  2. Joel P. Golden
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  3. Chris A. Rowe
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  4. Brian D. MacCraith
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  5. Frances S. Ligler
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Feldstein, M.J., Golden, J.P., Rowe, C.A. et al. Array Biosensor: Optical and Fluidics Systems. Biomedical Microdevices 1, 139–153 (1999). https://doi.org/10.1023/A:1009900608757

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  • DOI: https://doi.org/10.1023/A:1009900608757

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