Abstract
The coat protein (CP) genes CP1, CP2 and CP3 of Rice tungro spherical virus (RTSV) were introduced individually or together to indica and/or japonica rice cells by particle bombardment and transgenic plants were produced. Plants derived from selfed progeny of the primary transformants were subjected to virus inoculation via leafhoppers, the natural vector of the virus. Sixteen out of the nineteen selected transgenic plant lines, as well as their R1, R2 and/or R3 progeny that contained the target gene, accumulated transcripts of the chimeric CP gene(s) by RNA blot analysis. We obtained evidence of moderate levels of protection to RTSV infection, ranging from 17% to 73% of seedlings that escaped infection and a significant delay of virus replication under greenhouse conditions in plant lines that expressed the RTSV-CP1, CP2 and CP3 genes singly or together. There was not an additive effect on resistance when more than one CP gene is expressed. This study is the first to report pathogen-derived resistance to infection by RTSV, one of the two viruses that are involved in rice tungro disease. It is also the first example of CP-mediated protection against a virus that contains more than one CP gene from the same virus.
Similar content being viewed by others
References
Baulcombe DC: Mechanisms of pathogen derived resistance to viruses in transgenic plants. Plant Cell 8: 1833–1844 (1996)
Beachy RN: Mechanisms and applications of pathogenderived resistance in transgenic plants. Curr Opin Biotechnol 8: 215–220 (1997).
Bendahmane M, Fitchen JH, Zhang G, Beachy RN: Studies of coat protein-mediated resistance to tobacco mosaic tobamovirus: correlation between assembly of mutant coat protein and resistance. J Virol 71: 7942–7950 (1997).
Cabauatan PQ, Hibino H: Transmission of rice tungro bacilliform and spherical viruses by Nephotettix virescens Distant. Philipp. Phytopathology 21: 103–109 (1985).
Chen L, Zhang S, Beachy RN, Fauquet C: A protocol for consistent, large scale production of fertile transgenic rice plants. Plant Cell Rep 18: 25–31 (1998).
Christensen AH, Quail P: Ubiquitin promoter-based vectors for high level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res 5: 213–218 (1996).
Cooley J, Ford T, Christou P: Molecular and genetic characterization of elite transgenic rice plants produced by electricdischarge particle acceleration. Theor Appl Genet 90: 97–104 (1995).
Dasgupta I, Hull R, Eastop S, Poggi PC, Blakebrough M, Boulton MI, Davies JW: Rice tungro bacilliform virus DNA independently infects rice after Agrobacterium mediated transfer. J Gen Virol 72: 1215–1221 (1991).
Dellaporta SL, Wood J, Hicks JB: A plant DNA minipreparation: version II. Plant Mol Biol Rep 14: 19–21 (1983).
Dougherty WG, Lindbo JA, Smith HA, Parks TD, Swaney S, Proebsting WM: RNA-mediated virus resistance in transgenic plants: exploitation of a cellular pathway possibly involved in RNA degradation. Mol. Plant-Microbe Interact 7: 544–552 (1994).
English JJ, Mueller E, Baulcombe DC: Suppression of virus accumulation in transgenic plants exhibiting silencing of nuclear genes. Plant Cell 8: 179–188 (1996).
Fitchen JH, Beachy RN: Genetically engineered protection against viruses in transgenic plants. Annu Rev Microbiol 47: 739–763 (1993).
Haffner MH, Chin MB, Lane BG: Wheat embryo ribonucleates. XII. Formal characterization of terminal and penultimate nucleoside residues at the 5′-ends of ‘capped’ RNA from imbibing wheat embryos. Can J Biochem 56: 729–733 (1978).
Hayakawa T, Zhu Y, Itoh K, Kimura Y, Izawa T, Shimamoto K, Toriyama S: Genetically engineered rice resistant to rice stripe virus, an insect transmitted virus. Proc Natl Acad Sci USA 89: 9865–9869 (1992).
Herdt RW: Research priorities for biotechnology. Rice Biotechnology. In: Khush GS, Toennissen GH (eds) Biotechnology in Agriculture, pp. 19–54. CAB International, Wallingford, UK (1991).
Herdt RW: Equity considerations in setting priorities for third world rice biotechnology. Dev Seeds Change 4–19 (1988).
Hibino H, Cabautan PQ: Infectivity neutralization of rice tungro-associated virses acquired by vector leafhoppers. Phytopathology 77: 473–476 (1987).
Ibrahim N, Chen YM: Enzyme-linked immunosorbent assay untuk penyakit merah virus padi. Department of Agriculture, Malaysia (1989).
Jones MC, Gough K, Dasgupta I, Subba Rao BL, Cliffe J, Qu R, Shen P, Kaniewska M, Blakebrough M, Davies JW, Beachy RN, Hull R: Rice tungro disease is caused by an RNA and a DNA virus. J Gen Virol 72: 757–761 (1991).
Lomonossoff GP: Pathogen-derived resistance to plant viruses. Annu Rev Phytopath 33: 323–343 (1995).
Nelson RS, McCormic SM, Delannay X, Dube P, Layton J, Anderson EJ. Kaniewska M, Proksch RK, Rogers SG, Fraley RT, Beachy RN: Virus tolerance, plant growth, and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/technology 6: 403–409 (1988).
Ngon A Yassi M: Caractérisation moléculaire du virus de la panachure jaune du riz (RYMV) et production de riz transgénique. Thèse de doctorat, Université Montpellier II, France (1993).
Powell-Abel P, Nelson RS, De B, Hoffman N, Rogers SG, Fraley RT, Beachy RN: Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743 (1986).
Qu R, de Kochko A, Zhang L, Marmey P, Li L, Tian W, Zhang S, Fauquet CM, Beachy RN: Analysis of a large number of independent transgenic rice plants produced by the biolistic method. In Vitro Cell Dev Biol Plant 32: 233–240 (1996).
Rogers SG, Klee HJ, Horsch RB, Farley RT: Improved vectors for plant transformation: expression cassette vectors and new selectable markers. Meth Enzymol 153: 253–277 (1987).
Sanford JC, Johnston SA: The concept of parasite derived resistance: deriving resistance genes from the parasite's own genome. J Theor Biol 115: 395–405 (1985).
Shen P, Kaniewska M, Smith C, Beachy RN: Nucleotide sequence and genomic organization of rice tungro spherical virus. Virology 193: 621–630 (1993).
Sivamani E, Shen P, Opalka N, Beachy RN, Fauquet CM: Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method. Plant Cell Rep 15: 322–327 (1996).
Sivamani E, Shen P, Wang J, Fauquet CM, Beachy RN: Rice transformation with cDNA constructs of coat protein genes of rice tungro spherical virus (RTSV) for resistance against the tungro disease. In: Proceedings of the Seventh Annual Meeting of the Rockefeller Foundation's International Program on Rice Biotechnology, Bali, Indonesia, p. 228 (1994).
Thomas PE, Kaniewski WK, Lawson EC: Reduced field spread of potato leafroll virus in potatoes transformed with the potato leafroll virus coat protein gene. Plant Dis 81: 1447–1453 (1997).
Van Kammen A: Virus-induced gene silencing in infected and transgenic plants. Trends Plant Sci 2: 409–411 (1997).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sivamani, E., Huet, H., Shen, P. et al. Rice plant (Oryza sativa L.) containing Rice tungro spherical virus (RTSV) coat protein transgenes are resistant to virus infection. Molecular Breeding 5, 177–185 (1999). https://doi.org/10.1023/A:1009633816713
Issue Date:
DOI: https://doi.org/10.1023/A:1009633816713