Abstract
Experiments with free cell biomass (cells + exopolysaccharides) ofRhizobium BJVr 12 (mungbean isolate) showed that amount ofCr3+ ion sorbed is influenced by the amount of biomass toCr3+ concentration ratio and time of contact. A ratio of 0.5 gfresh biomass to 10.0 ml 5.03 ppm Cr3+ sorbed 0.0275 mg Crequivalent to an uptake of 2.86 mg Cr g-1 dry biomass and 1.0g: 10.0 ml sorbed 0.0366 mg Cr equivalent to an uptake of 1.9 mg Crg-1 biomass. Immobilized cell biomass in ceramic beads and inaquacel (a porous cellulose carrier with a charged surface) were moreefficient than free cell biomass in adsorbing Cr(III). A reduction of49.7percnt; of Cr(III) for free cells, 95.6% for cells immobilized inceramic beads and 94.6% for cells in aquacel was achieved after 48hours under shaken conditions. Sorption capacities of immobilized cellbiomass in ceramic beads and aquacel ranged from 5.01 to 5.06 mg Crg-1 dry cell biomass. The biosorption of Cr3+follows generally the Langmuir and Freundlich models of adsorption at lowCr3+ concentrations. The Langmuir constant for immobilizedcells in ceramic beads are: Q0, 0.065 mmol Crg-1 biomass; b (affinity constant), - 694 lmmol-1 Cr and for cells in aquacel Q, 0.07 mmol Crg-1 biomass; b, - 694 l mmol Cr g-1 Cr. TheFreundlich constants are: K, 0.071 mmol Cr g-1 biomass; n,0.13 g-1 biomass l-1 and for aquacel: K, 0.074mmol g-1 biomass; n, 0.13 g-1 biomass. Biotrapsmade up of immobilized cells in ceramic beads and aquacel were tested foradsorbing Cr(III) using two different flow rates: 0.5 ml/min and 1.5 ml/min.A significantly higher amount of Cr(III) was adsorbed at the lower flow rateof 0.5 ml/min. Biosorption of Cr3+ is competitive. Thetreatment of a waste water sample containing 6.03 ppm Cr3+ andother cations with the biomass reduced the Cr3+ concentrationto that much lower than for the test solution containing only Cr. Recoveryof biosorbed Cr(III) was by treatment at a different pH using dilute HClsolution. Recovery was higher for cells imbibed in ceramic beads thanaquacel. Percentage recoveries for cells in aquacel are 46.4% at pH1.0, 33.0% at pH 3.0 and 6.6% at pH 6.0–7.0. For cellsin ceramic beads, percentage recoveries are: 93.1% at pH 1.0,75.6% at pH 3.0 and 16.4% at pH 6.0–7.0. Biosorption ofCr3+ by cells immobilized in ceramic beads is reversible butonly partially for cells in aquacel.
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Mamaril, J.C., Paner, E.T. & Alpante, B.M. Biosorption and desorption studies of chromium (III) by free and immobilized Rhizobium (BJVr 12) cell biomass. Biodegradation 8, 275–285 (1997). https://doi.org/10.1023/A:1008213712910
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DOI: https://doi.org/10.1023/A:1008213712910