Abstract
A bitter controversy had existed as to the minimum number of quanta required for the evolution of one molecule of oxygen in photosynthesis: Otto Warburg had insisted since 1923 that this value was 3–4, whereas Robert Emerson and others continued to obtain a value of 8–12 since the 1940s. It is shown in this letter that the 1931 Nobel-laureate of Physiology & Medicine Otto Warburg published, in his last and final paper, just before his death in 1970, a measured minimum quantum requirement of oxygen evolution of 12 at the lowest intensities of light he used. Although using his theory on photolyte, Warburg calculated a value of 3–4 for the quantum requirement, this is the first confirmation by Warburg of the higher measured quantum requirement. However, it has remained unknown to most investigators. It is expected that this information will be of general interest not only to those interested in the history and research on photosynthesis, but to the entire sci entific community, especially the writers of text books in biology, biochemistry and biophysics.
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References
Arnold W (1935) Investigations in Photosynthesis. PhD thesis, Harvard University, Cambridge, MA, USA, 48 pp
Arnold W(1949) A calorimetric determination of the quantum yield in photosynthesis. In: Franck J and Loomis WE (eds) Photosynthesis in Plants, pp 273–276. The Iowa State College Press, Ames, IA
Arnold WA (1991) Experiments. Photosynth Res 27: 73–82
Duysens LNM (1989) The discovery of the two photosynthetic systems: a personal account. Photosynth Res 21: 61–79
Emerson R (1927) Ñber die Wirkung von Blausauere, Schwefel-Wasserstoff und Kohlenoxyd auf die Atmung versscheidner Algen. 27 pages. Inaugaral Dissertation zur Erlangung Doktorwuerde. Philosophischen Fakültat der Friedrich-Wilhelms-Universität zu Berlin, 11 October, 1927, Druck von Paul Berlin SW 48
Emerson R (1958) The quantum yield of photosynthesis. Ann Rev Plant Physiol 9: 1–24
Emerson R and Arnold WA (1932a) A separation of the reactions in photosynthesis by means of intermittent light. J Gen Physiol 15: 391–420
Emerson R and Arnold WA (1932b) The photochemical reaction in photosynthesis J Gen Physiol 16: 191–205
Emerson R and Lewis CM (1939) Factors influencing the efficiency of photosynthesis. Am J Bot 26: 808–822
Emerson R and Lewis CM (1941) Carbon dioxide exchange and the measurement of quantum yield of photosynthesis. Am J Bot 28: 789–804
Emerson R and Lewis CM (1943a) Factors influencing the efficiency of photosynthesis. Am J Bot 26: 808–822
Emerson R and Lewis CM (1943b) The dependence of quantum yield of Chlorella photosynthesis on wavelength of light. Am J Bot 30: 165–178
Emerson R, Chalmers RV and Cederstrand CN (1957) Some factors influencing the longwave limit of photosynthesis. Proc Natl Acad Sci USA 43: 133–143
Govindjee R, Rabinowitch E and Govindjee (1968) Maximum quantum yield and action spectrum of photosynthesis and fluorescence in Chlorella. Biochim Biophys Acta 162: 539–544
Hill R and Bendall F (1960) Function of two cytochrome components in chloroplats: a working hypothesis. Nature 186: 136–137
Joliot P, Barbieri G and Chabaud R (1969) Un nouveau modèle des centres photochimiques du système II. Photochem Photobiol 10: 309–329
Malkin S and Fork DC (1996) Bill Arnold and calorimetric measurements of the quantum requirement of photosynthesis – once again ahead of (his) time. Photosynth Res 48: 41–46
Manning WM, Stauffer JF, Duggar BM and Daniels F (1938) Quantum efficiency of photosynthesis in Chlorella. J Am Chem Soc 60: 266–274
Myers J (1994) The 1932 experiments. Photosynth Res 40: 303–310
Rabinowitch E (1961) Robert Emerson. Natl Acad Sci USA Biographical Memoirs. XXXV: 112–131
Walker D (1993) Energy, Plants and Man. Oxygraphics, East Sussex, UK
Warburg E (1918) Ñber die Energieumsatz bei photochemischen. Vorgaengen in Gasen VII. Photolyse die Jodwasserstoffs. Sitzungsberichte Preuss Akad Wissensch 26: 300–317
Warburg E and Rump W (1928) Ueber die Photolyse von Jodwasserstoffloesungen in Hexan und in Wasser. Z Physik 47: 305–322
Warburg O (1958) Photosynthesis experiments at the Max Planck Institute for Cell Physiology, Berlin-Dahlem. Science 128: 68–74
Warburg O (1963) The photolyte of photosynthesis. In: La Photosynthese (President du Colloque, Wurmser MR), pp 221–228. Editions du Centre National de la Recherche Scientifique, Paris
Warburg O and Negelein E (1923) Ñber den Einfluss der Wellenlänge auf den Energie Umsatz bei der Kohlensaureassimilationen. Z Physik Chem (Leipzig) 106: 191–218
Warburg O, Krippahl G and Lehman A (1969) Chlorophyll catalysis and Einstein's law of photochemical equivalence in photosynthesis. Am J Bot 56: 961–971
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Govindjee On the requirement of minimum number of four versus eight quanta of light for the evolution of one molecule of oxygen in photosynthesis: A historical note. Photosynthesis Research 59, 249–254 (1999). https://doi.org/10.1023/A:1006122501487
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DOI: https://doi.org/10.1023/A:1006122501487