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Estimating the contribution of Photosystem I to total leaf chlorophyll fluorescence

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Abstract

Chlorophyll a fluorescence characteristics were investigated in 12 species and 2 hybrids from the genus Flaveria exhibiting C3, C3–C4 intermediate, or C4 photosynthesis, and in the C4 species Zea mays. At room temperature, the variable fluorescence divided by the maximum fluorescence (FV/FM) of dark-adapted leaves decreased from C3 to C4 plants. This trend was qualitatively paralleled by an increase of the 735 nm peak relative to the 685 nm peak (F735/F685) of fluorescence emission spectra measured at low temperature (77 K). The variations were analysed using a quantitative model that takes into account higher PS I fluorescence in C4 plants than in C3 plants. The model predicts a linear correlation between 1/(FV/FM) and F735/F685, and was experimentally confirmed. From linear regression analysis, the FV/FM of PS II was calculated to be 0.88. By comparing the FV/FM of PS II with the FV/FM from leaves, the PS I contribution to total F0 fluorescence at wavelengths greater than 700 nm was determined to be about 30% and 50% in C3 and C4 plants, respectively. The corresponding values for the FM fluorescence were 6% and 12%. It is concluded that the effects of PS I fluorescence are significant and should be taken into account when analysing fluorescence data.

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References

  • Adams III WW, Demmig-Adams B, Winter K and Schreiber U (1990) The ratio of variable to maximum chlorophyll fluorescence from Photosystem II, measured in leaves at ambient temperature and at 77K, as an indicator of the photon yield of photosynthesis. Planta 180: 166–174

    Google Scholar 

  • Barber J, Malkin S and Telfer A (1989) The origin of chlorophyll fluorescence in vivo and its quenching by the Photosystem II reaction centre. Philos Trans R Soc London Ser B: 323: 227–239

    Google Scholar 

  • Bassi R (1985) Spectral properties and polypeptide composition of the chlorophyll-proteins of from thylakoids of granal and agranal chloroplasts of maize (Z. mays L.). Carlsberg Res Commun 50: 127–143

    Google Scholar 

  • Bilger W and Björkman O (1990) Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. Photosynth Res 25: 173–185

    Google Scholar 

  • Bradbury M and Baker NR (1981) Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of Photosystem II electron acceptors and fluorescence emission from Photosystem I and II. Biochim Biophys Acta 63: 542–551

    Google Scholar 

  • Briantais J-M, Vernotte C, Krause GH and Weis E (1986) Chlorophyll a fluorescence of higher plants: chloroplasts and leaves. In: Govindjee, Amesz J and Fork DJ (eds) Light Emission by Plants and Bacteria, pp 539–583. Academic Press, New York

    Google Scholar 

  • Brugnoli E, De Tullio MC, Monteverdi MC, Lauteri M and Scartazza A (1995) Xanthophyll cycle components and energy dissipation in sun and shade leaves of C3 and C4 plants. In: Mathis P (ed) Photosynthesis: From Light to Biosphere, Vol IV, pp 79–82. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Butler WL (1978) Energy distribution in the photochemical apparatus of photosynthesis. Ann Rev Plant Physiol 29: 345–378

    Google Scholar 

  • Croce R, Zucchelli G, Garlaschi FM, Bassi R and Jennings RC (1996) Excited state equilibrium in the Photosystem I-light-harvesting I complex: P700 is almost isoenergetic with its antenna. Biochemistry 35: 8572–8579

    Google Scholar 

  • Dau H (1994) Molecular mechanisms and quantitative models of variable Photosystem II fluorescence. Photochem Photobiol 60: 1–23

    Google Scholar 

  • Dekker JP, Hassoldt A, Pettersson Å, van Roon H, Groot M-L and van Grondelle R (1995) On the nature of the F695 and F685 emission of Photosystem II. In Mathis P (ed) Photosynthesis: From Light to Biosphere. Vol I, pp 53–56. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Demmig-Adams B (1990) Carotenoids and photoprotection in plants: A role for the xanthophyll zeaxanthin. Biochim Biophys Acta 1020: 1–24

    Google Scholar 

  • Edwards G and Walker D (1983) C3, C4: Mechanisms, and Cellular and Environmental Regulation, of Photosynthesis. Blackwell Scientific Publications, Oxford

    Google Scholar 

  • Falbel TG, Staehelin LA and Adams III WW (1994) Analysis of xanthophyll cycle carotenoids and chlorophyll fluorescence in light intensity-dependent chlorophyll-deficient mutants of wheat and barley. Photosynth Res 42: 191–202

    Google Scholar 

  • Genty B, Briantais J-M and Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990: 87–92

    Google Scholar 

  • Genty B, Wonders J and Baker NR (1990) Non-photochemical quenching of F0 in leaves is emission wavelength dependent: Consequences for quenching analysis and its interpretation. Photosynth Res 26: 133–139

    Google Scholar 

  • Gilmore AM (1997) Mechanistic aspects of xanthophyll cycle-dependent photoprotection in higher plant chloroplasts and leaves. Physiol Plant 99: 197–209

    Google Scholar 

  • Govindjee (1995) Sixty-three years since Kautsky: Chlorophyll a fluorescence. Aust J Plant Physiol 22: 131–160

    Google Scholar 

  • Hatch MD (1987) C4 photosynthesis: A unique blend of modified biochemistry, anatomy and ultrastructure. Biochim Biophys Acta 895: 81–106

    Google Scholar 

  • Holzwarth AR (1991) Excited-state kinetics in chlorophyll systems and its relationship to the functional organization of the photosystems. In: H Scheer (ed) Chlorophylls, pp 1125–1151. CRC Press, Boca Raton, FL

    Google Scholar 

  • Horton P and Ruban AV (1994) The role of light-harvesting complex in energy quenching. In: Baker NR and Bowyer JR (eds) Photoinhibition of Photosynthesis, pp 111–128. Bios Scientific Publishers, Oxford

    Google Scholar 

  • Klughammer C and Schreiber U (1994) An improved method, using saturating light pulses, for the determination of Photosystem I quantum yield via P700+-absorbance changes at 830 nm. Planta 192: 261–268

    Google Scholar 

  • Koroleva OY, Brüggemann W and Krause GH (1994) Photoinhibition, xanthophyll cycle and in vivo chlorophyll fluorescence quenching of chilling-tolerant Oxyria digyna and chillingsensitive Zea mays. Physiol Plant 92: 577–584

    Google Scholar 

  • Krause GH and Weis E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Physiol Plant Mol Biol 42: 313–349

    Google Scholar 

  • Krause GH, Laasch H and Weis E (1988) Regulation of thermal dissipation of absorbed light energy in chloroplasts indicated by energy-dependent fluorescence quenching. Plant Physiol Biochem 26(4): 445–452

    Google Scholar 

  • Ku MSB, Jingrui W, Dai Z, Scott RA, Chu, C and Edwards GE (1991) Photosynthetic and photorespiratory characteristics of Flaveria species. Plant Physiol 96: 518–528

    Google Scholar 

  • Machold O (1991) The structure of light-harvesting complex II as deduced from its polypeptide composition and stoichiometry. I: Studies with Vicia faba. J Plant Physiol 138: 678–684

    Google Scholar 

  • Melis A (1991) Dynamics of photosynthetic membrane composition and function. Biochim Biophys Acta 1058: 87–106

    Google Scholar 

  • Melis A (1996) Excitation energy transfer: functional and dynamic aspects of Lhc (cab) proteins. In: Ort DR and Yocum CF (eds) Oxygenic Photosynthesis: The Light Reactions, pp 523–538. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Mukerji I and Sauer K (1989) Temperature-dependent steady-state and picosecond kinetic fluorescence measurements of a Photosystem I preparation from spinach. In: Briggs WR (ed) Photosynthesis, pp 105–122. Alan R. Liss, Inc, New York

    Google Scholar 

  • Owens TG (1994) Excitation energy transfer between chlorophylls and carotenoids. A proposed molecular mechanism for non-photochemical quenching. In: Baker NR and Bowyer JR (eds) Photoinhibition of Photosynthesis, pp 95–107. Bios Scientific Publishers, Oxford

    Google Scholar 

  • Peterson RB (1994) Regulation of electron transport in Photosystem I and II in C3, C3–C4, and C4 species of Panicum in response to changing irradiance and O2 levels. Plant Physiol 105: 349–356

    Google Scholar 

  • Pfündel E (1995) PS I fluorescence at room temperature: possible effects on quenching coefficients. In: Mathis P (ed) Photosynthesis: From Light to Biosphere, Vol II, pp 855–858. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Pfündel E and Meister A (1996) Flow cytometry of mesophyll and bundle sheath chloroplast thylakoids of maize (Zea mays L.). Cytometry 23: 97–105

    Google Scholar 

  • Pfündel E and Pfeffer M (1997) Modification of Photosystem I light-harvesting occurred during the evolution of (NADP-malic enzyme) C4 photosynthesis. Plant Physiol 114: 145–152

    Google Scholar 

  • Pfündel E, Nagel E and Meister A (1996) Analyzing the light energy distribution in the photosynthetic apparatus of C4 plants using highly purified mesophyll and bundle sheath thylakoids. Plant Physiol 112: 1055–1070

    Google Scholar 

  • Quick WP and Horton P (1984) Studies on the induction of chlorophyll fluorescence quenching by redox state and transthylakoid pH gradient. Proc R Soc London Ser B 220: 371–382

    Google Scholar 

  • Quick WP and Stitt M (1989) An examination of factors contributing to non-photochemical quenching of chlorophyll fluorescence in barley leaves. Biochim Biophys Acta 977: 287–296

    Google Scholar 

  • Roelofs TA, Lee C-H and Holzwarth AR (1992) Global target analysis of picosecond chlorophyll fluorescence kinetics from pea chloroplasts. A new approach to the characterization of the primary processes in Photosystem II α-and β-units. Biophys J 61: 1147–1163

    Google Scholar 

  • Satoh K and Fork DC (1983) A new mechanism for adaptation to changes in light intensity and quality in the red alga Porphyra perforata. III. Fluorescence transients in the presence of 3-(3,3-dichlorophenyl)-1,1-dimethylurea. Plant Physiol 71: 673–676

    Google Scholar 

  • Schatz G, Brock H and Holzwarth AR (1988) Kinetic and energetic model for the primary processes in Photosystem II. Biophys J 54: 397–405

    Google Scholar 

  • Schreiber U, Bilger W and Neubauer C (1994) Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze E-D and Caldwell mm (eds) Ecophysiology of Photosynthesis, pp 49–70. Springer-Verlag, Berlin

    Google Scholar 

  • Schreiber U, Schliwa U and Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10: 51–62

    Google Scholar 

  • Siefermann-Harms D (1985) Carotenoids in photosynthesis. I. Location in photosynthetic membranes and light-harvesting function. Biochim Biophys Acta 811: 325–355

    Google Scholar 

  • Thayer SS and Björkman O (1992) Carotenoid distribution and deepoxidation in thylakoid pigment–protein complexes from cotton leaves and bundle-sheath cells of maize. Photosynth Res 33: 213–225

    Google Scholar 

  • van Grondelle R, Dekker JP, Gillbro T and Sundstrom V (1994) Energy transfer and trapping in photosynthesis. Biochim Biophys Acta 1187: 1–65

    Google Scholar 

  • Vogelmann TC (1993) Plant tissue optics. Annu Rev Physiol Plant Mol Biol 44: 231–251

    Google Scholar 

  • Walker DA (1992) Concerning oscillation. Photosynth Res 34: 387–395

    Google Scholar 

  • Walz H (1994) New emitter-detector unit ED-101BL with blue measuring light for the PAM fluorimeter. Walz News 4: 4

    Google Scholar 

  • Weis E (1985) Chlorophyll fluorescence at 77K in intact leaves: characterization of a technique to eliminate artifacts related to self-absorption. Photosynth Res 6: 73–86

    Google Scholar 

  • Weis E and Berry JA (1987) Quantum efficiency of Photosystem II in relation to 'energy'-dependent quenching of chlorophyll fluorescence. Biochim Biophys Acta 894: 198–208

    Google Scholar 

  • Wittmershaus BP, Woolf VM and Vermaas WFJ (1992) Temperature dependence and polarization of fluorescence from Photosystem I in the cyanobacteria Synechocystis sp. PCC 6803. Photosynth Res 31: 75–87

    Google Scholar 

Download references

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Authors and Affiliations

  1. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstraße 3, D-06466, Gatersleben, Germany

    Erhard Pfündel

  2. Universität Würzburg, Lehrstuhl Botanik II, Julius-von-Sachs-Platz 3, 97082, Würzburg, Germany

    Erhard Pfündel

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Pfündel, E. Estimating the contribution of Photosystem I to total leaf chlorophyll fluorescence. Photosynthesis Research 56, 185–195 (1998). https://doi.org/10.1023/A:1006032804606

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