Abstract
Plant regeneration via tissue culture varies with the genotype and is an important factor in establishing cell selection and genetic transformation systems. To select genotypes – especially Japanese ones – with a high regeneration capability, we screened 107 wheat genotypes (78 domestic, 29 foreign) for callus induction and regeneration capability from anther and immature embryo cultures.
For anther culture, 83 of 107 genotypes tested induced calli and 45 regenerated plants. Only 9 genotypes, however, produced green plants, 25 produced only albino plants, and 11 produced both green and albino plants. Glennson 81 was the highest in callus induction, followed by Orofen, Danchi–komugi and Chris. The genotypes with a relatively high regeneration capability were Framala 80 at 24% and Glennson 81 at 19%, these two genotypes produced only green plants. For immature embryo culture, 97 genotypes showed a 90% callus induction rate and 74 genotypes regenerated plants. Very few genotypes produced albino plants. The genotypes with a high regeneration capability were Genaro 81 at 90%, Chinese Spring at 80%, and Norin 75 at 75%.
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References
Becker D, Brettschneider R & Lorz H (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant Journ, 5: 299-307
Bullock WP, Baenziger PS, Schaeffer GW & Bottino PJ (1982) Anther culture of wheat (Triticum aestivumL.) F1's and their reciprocal crosses. Theor. Appl. Genet. 65: 155-159
Buyser J & Henry Y (1979) Androgenese sur des Bles tendres en cours de selection I. L'obtension des plantes in vitro. Z. Pflanzenzuecht 83: 49-56
Chin JC & Scott KJ (1977) Studies on the formation of roots and shoots in wheat callus cultures. Ann. Bot. 41: 473-481
Chu CC, Wang CC, Sun CS, Chien NP, Yin KC & Hsu C (1973) Investigation on the induction and morphogenesis of wheat (Triticum vulgare) pollen plants. Acta Botanica Sinica 15: 1-11
Henry Y & De Buyser J (1990) Wheat anther culture. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol. 13 (pp 285-350). Wheat. Springer Verlag, Berlin Heidelberg.
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497
Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M & Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant Journ. 5: 285-297
Ouyang J, Hu H, Chuang CC & Tseng CC (1973) Induction of pollen plants from anthers of Triticum aestivumL. cultured in vitro. Sci. Sin. 16: 79-95
Vasil V, Castillo AM, Fromm ME & Vasil IK (1992) Herbicide-resistant fertile transgenic wheat plants obtained by micro-projectile bombardment of regeneratable embryogenic callus. Bio/Technol. 10: 667-674
Vasil V, Srivastava V, Castillo AM, Fromm ME & Vasil IK (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technol. 11: 1553-1558
Wang P & Chen Y (1986) A study on the application of C17 medium for anther culture. Acta Botanica Sinica 28: 38-45
Weeks JT, Anderson OD & Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol. 102: 1077-1084
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Machii, H., Mizuno, H., Hirabayashi, T. et al. Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cultures. Plant Cell, Tissue and Organ Culture 53, 67–74 (1998). https://doi.org/10.1023/A:1006023725640
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DOI: https://doi.org/10.1023/A:1006023725640