Abstract
Aromatic amines were analysed by normal phase and reverse phase high performance liquid chromatography employing silica gel and octadecyl silane (ODS) columns, respectively. A number of mobile phases were used. The results obtained on silica gel column were compared with those achieved on reverse phase column. Excellent results were obtained on ODS and shoulder peaks were eliminated when a mixture of methyl alcohol and sodium perchlorate was used as mobile phase.
Similar content being viewed by others
References
Ajmal, M., Mohammad, A. and Anwar, S.: 1990a, ‘Separation of aromatic amines on thin layer plats inpregnated with picric acid’, J. Planar. Chromatogr. 3, 511–514.
Ajmal, M., Mohammad, A. and Anwar, S.: 1990b, ‘Separation of diphenyl amine from carbazole, Indole and p-dimethylamino benzaldehyde by TLC’, J. Planar. Chromatogr. 3, 336–339.
Ajaml, M., Mohammad, A. and Anwar, S.: 1990c, ‘Some observations on thin layer chromatography for identification and separation of amino compounds on mixed adsorbents with benzene containing eluents’, Microchem. J. 42, 206–217.
Dhillon, R. S., Singh, J., Gautam, V. K. and Chhobra, B. R.: 1988, ‘Detection of aminophenols of aromatic amines on thin-layer plates containing nitrite’, J. Chromatogr. 435, 256–257.
Eaton, J. L. and Mullins, D. E.: 1988, ‘Quantitative high performance thin layer chromatography of dansyl derivatives of biogenic amines’, Anal. Biochem. 172, 484–487.
Evans, C. H. S., Gray, S. and Kazim, N. O.: 1988, ‘Analysis of commercially available cheeses for the migraine inducer tyramine by thin layer chromatography and spectrophotometry’, Analyst 113.
Ewing, G. W.: 1985, Instrumental Methods of Chemical Analysis, 5th ed., McGraw-Hill, Singapore, pp. 246.
Hunt, D. C. and Grosby, M. T.: 1977, ‘Pthalimidopropylsilane. A new chemically bonded stationary phase for the determination of polynuclear aromatic hydrocarbons by high performance liquid chromatography’, J. Chromatogr. 130, 320–323.
Kikuchi, M., Tokai, A. and Yoshida, T.: 1986, ‘Determination of trace levels of linear alkyl benzene sulfonates in the marine environment by high performance liquid chromatography’, Water Res. 20, 643–650.
Matsueda, T., Osaki, Y. and Shigee, S.: 1982, ‘Determination of alkyl benzene sulphonate in water by high performance liquid chromatography’, Bunseki Kagaku 31, 59–63.
Mohammad, A., Ajmal, M. and Anwar, S.: 1995b, ‘Sorption behaviour of aromatic amines on impregnated silica gel layers’, J. Chromatogr. Sci. 33, 383–385.
Mahammad, A., Ajmal, M. and Anwar, S.: 1995, ‘Selective separation of p-Dimethylaminobenzaldehyde from some secondary and tertiary amines’, J. Planar. Chromatogr. 8, 216–218.
Nakae, A. and Kunihiro, K.: 1978, ‘Separation of homologous alkybenzenesulphonates by highperformance liquid chromatography’, 152, 137–144.
Organ, K., Katz, E. and Slavin, W.: 1979, ‘Determination of polycyclic aromatic hydrocarbons in aqueous samples by reversed phase liquid chromatography’, Anal. Chem. 51, 1315–1320.
Tanaka, Y. and Nakanishi, H.: 1981, ‘Purification on sephadex LH-20 and high performance liquid chromatographic determination of dodecylbenzene sulphonate in sediment and biological samples’, Bunseki Kagaku, 30, 569–573.
Thruston, A. D.: 1978, ‘High pressure liquid chromatography techniques for the isolation and identification of organics in drinking water extracts’, J. Chromatogr. Sci. 16, 254–259.
Yoshikawad, S., Sano, H. and Harada, T.: 1984, ‘Determination as LAS in river water by high peformance liquid chromatography’, Jap. J. Water Pollut. Res. 7, 191–194
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rao, R.A.K., Ajmal, M., Siddiqui, B.A. et al. Separation and determination of trace levels of aromatic amines by HPLC. Environ Monit Assess 54, 289–299 (1999). https://doi.org/10.1023/A:1005999418810
Issue Date:
DOI: https://doi.org/10.1023/A:1005999418810