Abstract
Removal of border cells from pea roots synchronizes and induces root cap cell division, wall biogenesis and differentiation. Three messages which are expressed differentially in such induced root caps have been cloned. Sequence analyses showed that the PsHRGP1-encoded protein has high homology with a hydroxyproline-rich glycoprotein. The PsCaP23-encoded protein has high homology with an alfalfa callus protein or translationally controlled human or mouse tumor protein P23. The PsRbL41-encoded protein has high homology with a highly basic 60S ribosomal protein L41. In situ hybridization showed that PsHRGP1, PsCaP23 and PsRbL41 messages are localized within dividing cells of the root cap. PsHRGP1 is highly expressed in uninduced root caps, but its message is repressed by 10–11 times as soon as cell division and differentiation begin. Expression of PsHRGP1 recovers to higher than (180%) its initial level in 30 min. PsHRGP1 is root-specific. PsCaP23 and PsRbL41 messages increase ca. 3-fold within 15 min after root cap induction. All three genes represent small families of 3–5 closely related genes in the pea genome.
References
Barlow PW: The root cap. In: Torrey JG, Clarkson DT (eds) The Development and Function of Roots, pp. 21–54. Academic Press, London (1975).
Brigham L, Woo H-H, Wen F, Hawes MC: Repression of meristem-specific mitosis and a global switch in gene expression in the root cap of pea (Pisum sativum L.) by endogenous signals. In press (1997).
Brigham L, Woo H-H, Hawes MC: Root border cells as tools in plant cell studies. Meth Cell Biol 49: 377–387 (1995).
Chen J, Varner JE: An extracellular matrix protein in plants: characterization of a genomic clone for carrot extensin. EMBO J 4: 2145–2151 (1985).
Chitpatima ST, Makrides S, Bandyopadhyay R, Brawerman G: Nucleotide sequence of a major messenger RNA for a 21 kilo-dalton polypeptide that is under translational control in mouse tumor cells. Nucl Acids Res 16: 2350 (1988).
Dayhoff OM: Atlas of Protein Sequence and Structure, Vol. 5, Suppl.3. National Biomedical Research Foundation, Washington, DC (1978).
de Almeida Engler J de, Van Montagu M, Engler G: Hybridization in situ of whole-mount messenger RNA in plants. Plant Mol Biol Rep 12: 321–331 (1994).
Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J: mRNA transcripts of several plant genes are polyadenylated at the multiple site in vivo. Nucl Acids Res 14: 2229–2240 (1986).
Feldman LJ: Regulation of root development. Annu Rev Plant Physiol 35: 223–242 (1984).
Feldman LJ: The development and dynamics of the root apical meristem. Am J Bot 71: 1308–1314 (1984).
Gross B, Gaestel M, Bohm H, Bielka H: cDNA sequences coding for a translationally controlled human tumor protein. Nucl Acids Res 17: 8367 (1989).
Hawes MC, Lin HJ: Correlation of pectolytic enzyme activity with the programmed release of cells from the root cap of Pisum sativum. Plant Physiol 94: 1855–1859 (1990).
Hong JC, Cheong YH, Nagao RT, Bahk JD, Cho MJ, Jey JL: Isolation and characterization of three soybean extensin cDNAs. Plant Physiol. 104: 793–796 (1994).
Kieliszwski MJ, Lamport DTA: Extensin: repetitive motifs, functional sites, post-translational codes, and phylogeny. Plant J 5: 157–172 (1994).
Klaudiny J, Kammer H, Scheit KH: Characterization by cDNA cloning of the mRNA of a highly basic human protein homologous to the yeast ribosomal protein YL41. Biochem Biophys Res Comm 187: 901–906 (1992).
Memelink J, Swords KMM, Kam RJ, Schilperoort RA, Hoge HC, Staehelin LA: Structure and regulation of tobacco extensin. Plant J 4: 1011–1022 (1993).
Otaka E, Higo K-I, Itoh T: Yeast ribosomal proteins. Mol Gen Genet 195: 544–546 (1984).
Parmentier Y, Durr A, Marbach J, Hirsinger C, Criqui MC, Fleck J, Jamet E: A novel wound-inducible extensin gene is expressed early in newly isolated protoplasts of Nicotiana sylvestris. Plant Mol Biol 29: 279–292 (1995).
Pay A, Heberle-Bors E, Hirt H: An alfalfa cDNA encodes a protein with homology to translationally controlled human tumor protein. Plant Mol Biol 19: 501–503 (1992).
Popham RA: Zonation of primary and lateral root apices of Pisum sativum. Ann Bot 42: 267–273 (1955).
Rougier M: Secretory activity of the root cap. In: Janner W, Loewis FA (eds) Encyclopedia of Plant Physiology: New Series, Plant Carbohydrates II, vol. 13B, pp. 542–574. Springer-Verlag, Berlin (1981).
Sauer N. Corbin DR, Keller B, Lamb CJ: Cloning and characterization of a wound-specific hydroxyproline-rich glycoprotein in Phaseolus vulgaris. Plant Cell Envir 13: 257–266 (1990).
Sievers A, Hensel W: Root cap, structure and function. In: Waisel Y, Eshel A, Kafkafi U. (eds) Plant Roots: The Hidden Half, pp. 53–73. Marcel Dekker, New York, Basel, Hong Kong. (1991).
Turley RB, Ferguson DL, Meredith WR Jr.: Isolation and characterization of a cDNA encoding ribosomal protein L41 from cotton (Gossypium hirsutum L.). Plant Physiol 105: 1449–1450 (1994).
Woo H-H, Brigham L, Hawes MC: Differential expression of genes during the externally induced root cap cell differentiation of Pisum sativum L. Cold Spring Harbor Laboratory Symposium: Signaling in Plant Development, p. 127 (1995).
Woo H-H, Brigham L, Hawes MC: Primary Structure of the mRNA encoding a 16.5-kDa ubiquitin-conjugating enzyme of Pisum sativum. Gene 148: 369–370 (1994).
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Woo, HH., Hawes, M.C. Cloning of genes whose expression is correlated with mitosis and localized in dividing cells in root caps of Pisum sativum L.. Plant Mol Biol 35, 1045–1051 (1997). https://doi.org/10.1023/A:1005930625920
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DOI: https://doi.org/10.1023/A:1005930625920