Abstract
Sorghum mitochondrial atp9 is polymorphic among male-sterile cytoplasms, but each cytoplasm is characterized by a major 650 nt transcript, regardless of fertility status. The gene is positioned 323 bp 3′ to trnfM. Primer extension revealed multiple atp9 5′ transcript termini, distributed from +1 to +28 3′ to trnfM; the termini could be labeled with polynucleotide kinase, suggesting that they result from the maturation of trnfM. Guanylyltransferase experiments, however, showed that four of the termini were capable. The juxtaposition of a putative promoter 3′ to trnfM results in a unique atp9 transcript population consisting of primary and processed transcripts.
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References
Binder S, Brennicke A: A tRNA gene transcription initiation site is similar to mRNA and rRNA promoters in plant mitochondria. Nucl Acids Res 21: 5012-5019 (1993).
Binder S, Hatzack F, Brennicke A: A novel pea mitochondrial in vitrotranscription system recognizes homologous and heterologous mRNA and tRNA promoter. J Biol Chem 270: 22182-22189 (1995).
Binder S, Marchfelder A, Brennicke A: Regulation of gene expression in plant mitochondria. Plant Mol Biol 32: 303-314 (1996).
Bonhomme S, Budar F, Lancelin D, Small I, Defrance MC, Pelletier G: Sequence and transcript analysis of the Nco2.5 Oguraspecific fragment correlated with cytoplasmic male sterility in Brassicacybrids. Mol Gen Genet 235: 340-348 (1992).
Coulthart MB, Spencer DF, Gray MW: Comparative analysis of a recombining-repeat-sequence family in the mitochondrial genomes of wheat (Triticum aestivumL.) and rye (Secale cerealeL.). Curr Genet 23: 255-264 (1993).
Dewey RE, Levings CS III, Timothy DH: Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell 44: 439-449 (1986).
Giese A, Thalheim C, Brennicke A, Binder C: Correlation of nonanucleotide motifs with transcript initiation of 18S rRNA genes in mitochondria of pea, potato and Arabidopsis. Mol Gen Genet 252: 429-436 (1996).
Itadani H, Wakasugi T, Sugita M, Sugiura M, Nakazono M, Hirai A: Nucleotide sequence of a 28-kbp portion of rice mitochondrial DNA: the existence of many sequences that correspond to parts to mitochondrial genes in intergenic regions. Plant Cell Physiol 35: 1239-1244 (1994).
Kaleikau EK, Andre CP, Walbot V: Sequence of the Fo-atpase (atp9) gene from rice mitochondria. Nucl Acids Res 18: 1990 (1990).
Makaroff CA, Apel IJ, Palmer JD: The atp6coding region has been disrupted and a novel reading frame generated in the mitochondrial genome of cytoplasmic male-sterile radish. J Biol Chem 264: 11706-11713 (1989).
Marchfelder A, Schuster W, Brennicke A: In vitroprocessing of mitochondrial and plastid derived rRNA precursors in a plant mitochondrial extract. Nucl Acids Res 18: 1401-1406 (1990).
Nakazono M, Tsutsumi N, Suguira M, Hirai A: A small repeated sequence contains the transcription initiation sites for both trnfMand rrn26in rice mitochondria. Plant Mol Biol 28: 343-346 (1995).
Parks DT, Dougherty WG, Levings CS III, Timothy DH: Identification of two methionine transfer RNA genes in the maize mitochondrial genome. Plant Physiol 76: 1079-1082 (1984).
Pring DR, Tang HV, Schertz KF: Cytoplasmic male sterility and organelle DNAs sorghum. In: Levings CS III, Vasil IK (eds) The Molecular Biology of Plant Mitochondria, pp. 461- 495. Kluwer Academic Publishers, Dordrecht, Netherlands (1995).
Rapp WD, Stern DB: A conserved 11 nucleotide sequence contains an essential promoter element of the maize mitochondrial atp1gene. EMBO J 11: 1065-1073 (1992).
Rapp WD, Lupold DS, Mack S, Stern DB: Architecture of the maize mitochondrial atp1promoter as determined by linker-scanning and point mutagenesis. Mol Cell Biol 13: 7232-7238 (1993).
Rapp WD, Stern DB: Mitochondrial transcription and translation. In: Levings CS III, Vasil IK (eds) TheMolecular Biology of Plant Mitochondria, pp. 185-205. Kluwer Academic Publishers, Dordrecht, Netherlands (1995).
Salazar RA, Pring DR, Kempken F: Editing of mitochondrial atp9 transcripts from two sorghum lines. Curr Genet 20: 483- 486 (1991).
Singh M, Brown GG: Suppression of cytoplasmicmale sterility by nuclear genes alters expression of a novel mitochondrial gene region. Plant Cell 3: 1349-1362 (1991).
Tang HV, Pring DR, Muza FR, Yan B: Sorghum mitochondrial orf25and a related chimeric configuration of a male-sterile cytoplasm. Curr Genet 29: 265-274 (1996).
Tang HV, Pring DR, Shaw LC, Salazar RA, Muza FR, Yan B, Schertz KF: Transcript processing internal to a mitochondrial open reading frame is correlated with fertility restoration in male-sterile sorghum. Plant J 10: 123-133 (1996).
Worstell JV, Kidd HJ, Schertz KF: Relationships among male-sterility inducing cytoplasms of sorghum. Crop Sci 24: 186- 189 (1984).
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Yan, B., Salazar, R. & Pring, D. Transcription initiation sites for sorghum mitochondrial atp9 are positioned immediately 3′ to trnfM. Plant Mol Biol 34, 823–829 (1997). https://doi.org/10.1023/A:1005814812438
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DOI: https://doi.org/10.1023/A:1005814812438