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Callus induction and plant regeneration in Miscanthus x ogiformis Honda 'Giganteus' as influenced by benzyladenine

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Abstract

Leaf sections of greenhouse-grown Miscanthus x ogiformis Honda 'Giganteus' plants and leaf sections or shoot apices of in vitro shoot cultures were grown on Murashige and Skoog medium containing various concentrations of benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid. On leaf sections, the callus induction decreased with increasing BA concentration. The percentage of embryogenic callus was increased, the percentage of root-forming callus decreased, and a new shoot-forming callus type was formed by inclusion of BA during callus induction. A higher percentage of shoot-forming callus was formed on shoot apices compared with leaf sections of in vitro-grown shoots when cultured on 0.4 µM BA. The largest number of plants per callus piece was regenerated from shoot-forming callus, but maintenance of the high regeneration capacity proved difficult.

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Authors and Affiliations

  1. Research Group Plant Breeding and Propagation, Department of Ornamentals, Research Centre Aarslev, Danish Institute of Plant and Soil Science, Kirstinebjergvej 10, DK-5792, Aarslev

    Karen Koefoed Petersen

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  1. Karen Koefoed Petersen
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Petersen, K.K. Callus induction and plant regeneration in Miscanthus x ogiformis Honda 'Giganteus' as influenced by benzyladenine. Plant Cell, Tissue and Organ Culture 49, 137–140 (1997). https://doi.org/10.1023/A:1005808329685

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  • DOI: https://doi.org/10.1023/A:1005808329685

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