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CeReS-18, a novel cell surface sialoglycopeptide, induces cell cycle arrest and apoptosis in a calcium-sensitive manner

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Abstract

Very few growth inhibitors have been identified whichcan inhibit the proliferation of a broad spectrumof human breast cancer cell lines. CeReS-18, anovel cell surface sialoglycopeptide growth inhibitor, can reversiblyinhibit the proliferation of both estrogen receptor positive(MCF-7) and negative (BT-20) human breast cancer celllines. In addition, at concentrations above those requiredfor the reversible inhibition of cell proliferation, CeReS-18can also induce cell death in MCF-7 cells.Changes in nuclear and cytoplasmic morphology, characteristic ofapoptosis, were detected in MCF-7 cells treated witha cytotoxic concentration of CeReS-18, and internucleosomal DNAcleavage was also observed. The sensitivity of MCF-7and BT-20 cells to the biological properties ofCeReS-18 could be influenced by altering the calciumconcentration in the extracellular growth medium, such thatwhen the calcium concentration in the environment wasdecreased, an increased sensitivity to CeReS-18-induced growth inhibitionand cytotoxicity were observed. The addition of thecalcium chelating agent EGTA to MCF-7 cells, culturedin a normal calcium environment, could mimic theincreased sensitivity to the biological effects of CeReS-18observed under reduced calcium conditions.

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Authors and Affiliations

  1. Center for Basic Cancer Research, Division of Biology, Kansas State University, Manhattan, Kansas, 66506, USA

    Natalie A. Betz, Heideh K Fattaey, Brenda A. Westhoff, Avelina Q. Paulsen & Terry C. Johnson

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  1. Natalie A. Betz
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  2. Heideh K Fattaey
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  3. Brenda A. Westhoff
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  4. Avelina Q. Paulsen
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  5. Terry C. Johnson
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Betz, N.A., Fattaey, H.K., Westhoff, B.A. et al. CeReS-18, a novel cell surface sialoglycopeptide, induces cell cycle arrest and apoptosis in a calcium-sensitive manner. Breast Cancer Res Treat 42, 137–148 (1997). https://doi.org/10.1023/A:1005735723808

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