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Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer–Villiger monooxygenase reactions

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Abstract

Cyclohexanone monooxygenase was produced from Acinetobacter calcoaceticus grown on a medium containing both glutamate (30 g l−1) and cyclohexanol (1 g l−1). Productivity was increased to 650 U l−1, an order of magnitude greater than previous production methods, thereby enhancing the potential commercial utility of this enzyme.

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Authors and Affiliations

  1. Department of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK

    Scott S. Barclay, John M. Woodley & Malcolm D. Lilly

  2. Process Research and Development Department, Pfizer Central Research, Pfizer Ltd, Ramsgate Road, Sandwich, Kent, CT13 9NJ, UK

    Peter L. Spargo & Alan J. Pettman

Authors
  1. Scott S. Barclay
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  2. John M. Woodley
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  3. Malcolm D. Lilly
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  4. Peter L. Spargo
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  5. Alan J. Pettman
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Barclay, S.S., Woodley, J.M., Lilly, M.D. et al. Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer–Villiger monooxygenase reactions. Biotechnology Letters 23, 385–388 (2001). https://doi.org/10.1023/A:1005610815727

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  • DOI: https://doi.org/10.1023/A:1005610815727

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