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Bleach-stable, alkaline protease from Bacillus sp.

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Abstract

A bleach-stable, thermotolerant, alkaline protease for detergent formulation from a newly isolated Bacillus SB5 is reported. Most (85%) activity of the enzyme was retained in the presence of 10% (v/v) H2O2 and 1% SDS (w/v) at 40 °C, after 1 h. The enzyme was optimal at pH 10 and 60 °C to 70 °C. Enzyme activity was enhanced 30 to 80% in presence of ionic and non-ionic detergents, surfactants and commercial detergents or bleach.

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Authors and Affiliations

  1. Department of Microbiology, University of Delhi, South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, 110 021, India

    Rani Gupta, Komal Gupta, R.K. Saxena & Seema Khan

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  1. Rani Gupta
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  2. Komal Gupta
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  3. R.K. Saxena
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  4. Seema Khan
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Gupta, R., Gupta, K., Saxena, R. et al. Bleach-stable, alkaline protease from Bacillus sp.. Biotechnology Letters 21, 135–138 (1999). https://doi.org/10.1023/A:1005478117918

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  • DOI: https://doi.org/10.1023/A:1005478117918

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