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Water-retentive polymers increase nodulation of actinorhizal plants inoculated with Frankia

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Abstract

Actinorhizal plants form a nodular, nitrogen-fixing root symbiosis with the actinomycete Frankia and are economically and ecologically important due to their ability to improve the nitrogen fertility of disturbed and infertile substrates. In this study, water-retentive polymer inoculum carriers were applied as a root dip. This treatment significantly increased nodulation and in some cases early growth of Alnus glutinosa (L.) Gaertn. and Casuarina equisetifolia var. equisetifolia Forst. & Forst. in a controlled environment and also of A. glutinosa under field conditions. Nodule number and nodule dry weight per plant were at least two to three times greater after 56 to 140 days for plants inoculated with Frankia carried in a water-retentive polymer base compared with plants inoculated with Frankia in water. Nodules on the roots of the plants that were inoculated with Frankia in a polymer slurry were distributed throughout the entire root system, rather than concentrated near the root collar. When amended with water-retentive polymers, actinorhizal plants inoculated with 5- to 10-fold lower titers of Frankia exhibited early growth and nodule numbers equal to or greater than those plants inoculated with standard titers without polymers. The water-retentive, superabsorbent polymers clearly increased the nodulation of two actinorhizal plant species.

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Authors and Affiliations

  1. Department of Natural Resources and Environmental Sciences, University of Illinois, W-503 Turner Hall, 1102, S. Goodwin, Urbana, Illinois, 61801, USA

    Steven J. Kohls & Jeffrey O. Dawson

  2. New Chemical Entities, Inc., 11804 North Creek Parkway South, Bothell, Washington, 98011-8805, USA

    Dwight D. Baker

  3. MiTech Research and Development Inc, 3522 Labore Rd., St. Paul, Minnesota, 55101, USA

    Douglas A. Kremer

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  1. Steven J. Kohls
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  2. Dwight D. Baker
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  3. Douglas A. Kremer
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  4. Jeffrey O. Dawson
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Kohls, S.J., Baker, D.D., Kremer, D.A. et al. Water-retentive polymers increase nodulation of actinorhizal plants inoculated with Frankia. Plant and Soil 214, 105–115 (1999). https://doi.org/10.1023/A:1004634804354

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