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Effects of nutrient solution zinc activity on net uptake, translocation, and root export of cadmium and zinc by separated sections of intact durum wheat (Triticum turgidum L. var durum) seedling roots

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Abstract

Cd accumulation in durum wheat presents a potential health risk to consumers. In an effort to understand the physiological mechanisms involved with Cd accumulation, this study examined the effects of Zn on Cd root uptake and phloem translocation in a split– root system. Durum wheat seedlings were grown in chelate-buffered nutrient solution with intact root systems divided into two sections. Each root section grew in a separate 1 l pot, one of which contained 0.2 μM CdSO4. In addition, each two-pot system contained ZnSO4 in the following combinations (in μm) (for -cd root system: +cd root system): 1:1, 1:10, 10:1,10:10, 1:19, and 19:1. Harvested plant material was analyzed for Cd and Zn. In addition, rates of Cd and Zn net uptake, translocation to the shoot, and root export (translocation from one root segment to the other) between days 8 and 22 were calculated. Results show that Zn was not translocated from one root section to its connected root section. Uptake rates of Cd increased as solution Zn concentrations increased. Cd translocation from one root section to the other decreased significantly when the Zn concentration in either pot was greater than 1 μM. These results show the potential of Zn to inhibit movement of Cd via the phloem, and suggests that providing adequate Zn levels may limit phloem loading of Cd into wheat grain. Increasing the rhizosphere activity of Zn2+ in Cd-containing soils may therefore result in reduced Cd accumulation in grain even while net Cd uptake is slightly enhanced.

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References

  • Anonymous 1994Status Report. Co-operative Risk Reduction Activities for Certain Dangerous Chemicals. Cadmium. Organization for Economic Co-operation and Development, Paris, France. p. 1–149.

  • Burau R G, Jopling W F, Martin C V and Snow G F 1996Monterey Basin Pilot Monitoring Project. University of California, Davis, California. p. A–1–M–46.

    Google Scholar 

  • Cataldo D A, Garland T R and Wildung R E 1983 Cadmium uptake kinetics in intact soybean plants. Plant Physiol 73, 844–848.

    PubMed  CAS  Google Scholar 

  • Choudhary M, Bailey L D and Grant C A 1994 Effect of zinc on cadmium concentration in the tissue of durum wheat. Can. J. Plant Sci. 74, 549–552.

    CAS  Google Scholar 

  • Codex Alimentarius Commission 1993 Risk Assessment Procedures Used by the Codex Alimentarius Commission, and its subsidiary and advisory bodies. Joint FAO/WHO Food Standards Programme, Rome, Twentieth Session, 28 June-7 July 1993, International Conference Centre, Geneva, Switzerland. p. 1–21.

  • Cutler J M and Rains D W 1974 Characterization of cadmium uptake by plant tissue. Plant Physiol. 54, 67–71.

    PubMed  CAS  Google Scholar 

  • Dudka S, Piotrowska M and Chlopecka A 1994 Effect of elevated concentrations of Cd and Zn in soil on spring wheat yield and the metal contents of the plants. Water Air Soil Poll. 76, 333–341.

    Article  CAS  Google Scholar 

  • Francis C W and Rush S G 1974 Factors affecting uptake and distribution of cadmium in plants. In Trace Substances in Environmental Health – VII. Ed. D D Hemphill. pp 75–81. University of Missouri, Columbia.

    Google Scholar 

  • Garrett R G 1994 The distribution of cadmium in A horizon soils in the prairies of Canada and adjoining United States. Current Research; Geological Survey of Canada 1994-B, 73–82.

  • Gerritse R G, van Driel W, Smilde K W and van Luit B 1983 Uptake of heavy metals by crops in relation to their concentration in the soil solution. Plant Soil 75, 393–404.

    Article  CAS  Google Scholar 

  • Graham R D, Ascher J S and Hynes S C 1992 Selecting zincefficient cereal genotypes for soils of low zinc status. Plant Soil 146, 241–250.

    Article  CAS  Google Scholar 

  • Grill E, Winnacker E-L and Zenk M H 1985 Phytochelatins: the principal heavy-metal complexing peptides of higher plants. Science 230, 674–676.

    CAS  PubMed  Google Scholar 

  • Holmgren G G S, Meyer M W, Chaney R L and Daniels R B 1993 Cadmium, lead, zinc, copper, and nickel in agricultural soils of the United States of America. J. Environ. Qual. 22, 335–348.

    Article  CAS  Google Scholar 

  • Homma Y and Hirata H 1978 Noticeable increase in cadmium absorption by zinc deficient rice plants.Soil Sci. Plant Nutr. 24, 295–297.

    Google Scholar 

  • Leopold I and Günther D 1997 Investigation of the binding properties of heavy-metal-peptide complexes in plant cell cultures using HPLC-ICP-MS. Fresenius J. Anal. Chem. 359, 364–370.

    Article  CAS  Google Scholar 

  • Lindsay W L 1991 Inorganic equilibria affecting micronutrients in soils. In Micronutrients in Agriculture. Eds. J J Mortvedt, F R Cox, L M Shuman and R M Welch. 2nd edition. pp 89–112. Soil Science Society of America, Madison, WI.

    Google Scholar 

  • Maitani T, Kubota H, Sato K and Yamada T 1996 The composition of metals bound to class III metallothionein (phytochelatin and its desglycyl peptide) induced by various metals in root cultures of Rubia tinctorum. Plant Physiol. 110, 1145–1150.

    PubMed  CAS  Google Scholar 

  • Marschner H 1995 Mineral Nutrition of Higher Plants. 2nd edition. Academic Press, London. 889 p.

    Google Scholar 

  • Norvell W A 1991 Reactions of metal chelates in soils and nutrient solutions. In Micronutrients in Agriculture. 2nd edition. Eds. J J Mortvedt, F R Cox, L M Shuman and R M Welch. pp 187–227. Soil Science Society of America, Madison, WI.

    Google Scholar 

  • Norvell W A and Welch R M 1993 Growth and nutrient uptake by barley (Hordeum vulgare L. cv Herta): Studies using an N-(2-hydroxyethyl)ethylenedinitrilotriacetic acid-buffered nutrient solution technique. I. Zinc ion requirements. Plant Physiol. 101, 619–625.

    PubMed  CAS  Google Scholar 

  • Oliver D P, Hannam R, Tiller K G, Wilhelm N S, Merry R H and Cozens G D 1994 Heavy metals in the environment: The effects of zinc fertilization on cadmium concentration in wheat grain. J. Environ. Qual. 23, 705–711.

    CAS  Google Scholar 

  • Oliver D P, Wilhelm N S, McFarlane J D, Tiller K G and Cozens G D 1997 Effect of soil and foliar applications of zinc on cadmium concentration in wheat grain. Aust. J. Exp. Agric. 37, 677–681.

    Article  CAS  Google Scholar 

  • Parker D R, Chaney R L and Norvell W A 1995a Chemical equilibrium models: Applications to plant nutrition research. Soil Sci Soc Am Special Pub. 42. Soil Sci. Soc. of Am., Am. Soc. Agron., Madison, WI. pp. 163–200.

    Google Scholar 

  • Parker D R, Norvell W A and Chaney R L 1995b GEOCHEM-PC-a chemical speciation program for IBM and compatible personal computers. Soil Sci. Soc. Am. Special Pub. 42. Soil Sci. Soc. of Am., Am. Soc. Agron., Madison, WI. pp. 253–269.

    Google Scholar 

  • Pearson J N and Rengel Z 1995 Uptake and distribution of 65Zn and 54Mn in wheat grown at sufficient and deficient levels of Zn and Mn. I. During vegetative growth. J. Exp. Bot. 46, 833–839.

    CAS  Google Scholar 

  • Salt D E and Rauser W E 1995 MgATP-dependent transport of phytochelatins across the tonoplast of oat roots. Plant Physiol. 107, 1293–1301.

    PubMed  CAS  Google Scholar 

  • Shuman LM 1991 Chemical forms of micronutrients in soils. In Micronutrients in Agriculture. 2nd edition. J J Mortvedt, F R Cox, L M Shuman and R M Welch. pp 113–144. Soil Science Society of America, Madison, WI.

    Google Scholar 

  • Smith R M and Martell A E 1989 Critical Stability Constants. Vol. 6: Second Supplement. Plenum Press, New York.

    Google Scholar 

  • Webb M J and Loneragan J F 1990 Zinc translocation to wheat roots and its implications for a phosphorus/zinc interaction in wheat plants. J. Plant Nutr. 13, 1499–1512.

    Article  CAS  Google Scholar 

  • Welch R M, Webb M J and Loneragan J F 1982 Zinc in membrane function and its role in phosphorus toxicity.In Plant Nutrition 1982. Proc. of the 9th Intl. Plant Nutrition Colloquium, Warwick University, England, August 22–27. Ed. A Scaife. pp 710-715. Commonwealth Agricultural Bureau, Farnham House, Farnham Royal, UK.

    Google Scholar 

  • Welch R M, Allaway W H, House W A and Kubota J 1991 Geographic distribution of trace element problems. In Micronutrients in Agriculture.2nd edition Eds. J J Mortvedt, F R Cox, L M Shuman and R M Welch. pp 31–57. Soil Science Society of America, Madison, WI.

    Google Scholar 

  • Welch R M and Norvell W A 1993 Growth and nutrient uptake by barley (Hordeum vulgare L. cv Herta): Studies using an N-(2-hydroxyethyl)ethylenedinitrilotriacetic acid-buffered nutrient solution technique. II. Role of zinc in the uptake and root leakage of mineral nutrients. Plant Physiol. 101, 627–631.

    PubMed  CAS  Google Scholar 

  • Welch R M 1995 Micronutrient nutrition of plants. Crit. Rev. Plant Sci. 14, 49–82.

    CAS  Google Scholar 

  • Williams C H and David D J 1976 The accumulation in soil of cadmium residues from phosphate fertilizers and their effect on the cadmium content of plants. Soil Sci. 121, 86–93.

    CAS  Google Scholar 

  • Williams R F 1948 The effects of P supply on the rates of intake of P and nitrogen and upon certain aspects of P metabolism in graminaceous plants. Aust. J. Sci. Res. (B)1, 333–361.

    CAS  Google Scholar 

  • Wolnik K A, Fricke F L, Capar S G, Braude G L, Meyer M W, Satzger R D and Bonnin E 1983 Elements in major raw agricultural crops in the United States. 1. Cadmium and lead in lettuce, peanuts, potatoes, soybeans, sweet corn, and wheat. J. Agric. Food Chem. 31, 1240–1244.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. U.S. Plant, Soil and Nutrition Laboratory, United States Department of Agriculture, Agricultural Research Service, USA

    R.M. Welch, J.J. Hart, W.A. Norvell & L.V. Kochian

  2. Department of Soil, Crop and Atmospheric Sciences, Cornell University, Ithaca, NY, 14853

    J.J. Hart

  3. Goizueta Business School, Emory University, Atlanta, GA, 30322, USA

    L.A. Sullivan

Authors
  1. R.M. Welch
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  2. J.J. Hart
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  3. W.A. Norvell
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  4. L.A. Sullivan
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  5. L.V. Kochian
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Welch, R., Hart, J., Norvell, W. et al. Effects of nutrient solution zinc activity on net uptake, translocation, and root export of cadmium and zinc by separated sections of intact durum wheat (Triticum turgidum L. var durum) seedling roots. Plant and Soil 208, 243–250 (1999). https://doi.org/10.1023/A:1004598228978

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