Abstract
Tetraploid wheatgrass, Thinopyrum junceiforme(2n = 4x = 28; J1J1J2J2), a wild relative of wheat, is an excellent source of resistance to Fusarium head blight. Intergeneric F1 hybrids (2n = 4x = 28; ABJ1J2) between durum wheat (Triticum turgidum; 2n = 4x = 28; AABB) cultivars Lloyd or Langdon and Th. junceiforme were synthesized. Most of the pairing in F1 hybrids was between the J1- and J2-genome chromosomes. Some pairing occurred between wheat chromosomes and alien chromosomes, resulting in segmental exchange that was confirmed by fluorescent in situ hybridization (FISH). The F1hybrids were largely male-sterile and were backcrossed, as the female parent, to the respective durum cultivar. Backcrosses from Lloyd × Th. junceiforme hybrids yielded fertile partial amphiploids (2n = 6x = 42; AABBJ1J2) as a result of functioning of unreduced female gametes of the hybrid. Lloyd proved to be a more useful durum parent than Langdon in crosses with Th. junceiforme designed to transfer scab resistance genes. Pairing in the amphiploids was characterized by preferential pairing,which resulted in bivalent formation. However, some intergeneric pairing also occurred. Several fertile hybrid derivatives were produced by further backcrossing and selfing. The introduction of alien chromatin into the durum complement was confirmed by FISH. Hybrid derivative lines had significantly lower mean infection scores (p = 0.01), the best showing 10.93% infection, whereas the parental durum cultivars had 70.34% to 89.46% infection. Hybridization with wild relatives may offer an excellent means of introducing scab resistance into durum wheat.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dewey, D.R., 1984. The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. Stadler Genet Symp 16: 209–279.
Heslop-Harrison, J.S., & T. Schwarzacher, 1996. Genomic Southern and in situ hybridization for plant genome analysis. In: P.P Jauhar (Ed.), Methods of Genome Analysis in Plants, pp. 163–179. CRC Press, Boca Raton, Florida.
Jasc Software, 1998. Paint Shop Pro Version 5, 336 pages. Jasc Software, Inc., Minnetonka, Minnesota.
Jauhar, P.P., 1990. Multidisciplinary approach to genome analysis in the diploid species, Thinopyrum bessarabicum and Th. elongatum (Lophopyrum elongatum), of Triticeae. Theor Appl Genet 80: 523–536.
Jauhar, P.P., 1991. Hybrid between durum wheat and diploid Thinopyrum bessarabicum. Genome 34: 283–287.
Jauhar, P.P., 1992. High chromosome pairing in hybrids between hexaploid bread wheat and tetraploid crested wheatgrass (Agropyron cristatum). Hereditas 116: 107–109.
Jauhar, P.P., 1993. Cytogenetics of the Festuca-Lolium Complex: Relevance to Breeding. Springer-Verlag, Heidelberg, Germany.
Jauhar, P.P., 1995. Meiosis and fertility of F1 hybrids between hexaploid bread wheat and decaploid tall wheatgrass (Thinopyrum ponticum). Theor Appl Genet 90: 865–871.
Jauhar, P.P. & A.B. Almouslem, 1998. Production and meiotic analyses of intergeneric hybrids between durum wheat and Thinopyrum species. In: A.A. Jaradat (Ed.), Proc Third International Triticeae Symposium, pp. 119–126. Scientific Publishers, Enfield, New Hampshire, USA.
Jauhar, P.P. & L.R. Joppa, 1996. Chromosome pairing as a tool in genome analysis: merits and limitations. In: P.P. Jauhar (Ed.), Methods of Genome Analysis in Plants, pp. 9–37. CRC Press, Boca Raton, Florida.
Jauhar, P.P. & T.S. Peterson, 1996. Thinopyrum and Lophopyrum as sources of genes for wheat improvement. Cereal Res Commun 24: 15–21.
Jauhar, P.P. & T.S. Peterson, 1998. Wild relatives of wheat as sources of Fusarium head blight resistance. Proc 1998 National Fusarium Head Blight (Scab) Forum, pp. 77–79. Michigan State University, East Lansing, Michigan, USA.
Jauhar, P.P, A.B. Almouslem, T.S. Peterson & L. R. Joppa, 1999. Inter-and intragenomic chromosome pairing in haploids of durum wheat. J Hered 90: 437–445.
Jauhar, P.P., O. Riera-Lizarazu, W.G. Dewey, B.S. Gill, C.F. Crane & J.H. Bennett, 1991. Chromosome pairing relationships among the A, B, and D genomes of bread wheat. Theor Appl Genet 82: 441–449.
Mujeeb-Kazi A., S. Roldan, D.Y. Suh, L.A. Sitch & S. Farooq, 1987. Production and cytogenetic analysis of hybrids between Triticum aestivum and some caespitose Agropyron species. Genome 29: 537–553.
Mukai, Y., 1996. Multicolor fluorescence in situ hybridization: A new tool for genome analysis. In: P.P. Jauhar (Ed.), Methods of Genome Analysis in Plants, pp. 163–179. CRC Press, Boca Raton, Florida.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Pienaar R.de V., G.M. Littlejohn & E.R. Sears, 1988. Genomic relationships in Thinopyrum. S Afr J Bot 54: 541–550.
Schwarzacher, T., K. Anamthawat-Jónsson, G.E. Harrison, A.K.M.R. Islam, J.Z. Jia, I.P. King, A.R. Leitch, T.E. Miller, S.M. Reader, W.J. Rogers, M. Shi & J.S. Heslop-Harrison, 1992. Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat. Theor Appl Genet 84: 778–786.
Sears, E.R., 1976. Genetic control of chromosome pairing in wheat. Ann Rev Genet 10: 31–51.
Stack, R.W. & M.P. McMullen, 1994. A visual scale to estimate severity of Fusarium head blight in wheat. North Dakota State University Extension Service, Publication, PP-1095.
Wang, R.R.-C., 1989. An assessment of genome analysis bases on chromosome pairing in hybrids of perennial Triticeae. Genome 32: 179–189.
Wang, R.R.-C., & C. Hsiao, 1989. Genome relationship between Thinopyrum bessarabicum and T. elongatum: revisited. Genome 32: 802–809.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jauhar, P.P., Peterson, T.S. Hybrids between durum wheat and Thinopyrum junceiforme: Prospects for breeding for scab resistance. Euphytica 118, 127–136 (2001). https://doi.org/10.1023/A:1004070006544
Issue Date:
DOI: https://doi.org/10.1023/A:1004070006544