Abstract
A novel H2O2 sensor is achieved by immobilizing horseradish peroxidase (HRP) in an electropolymerized methylene green (PMG) redox film. The electropolymerization of MG is carried out in a neutral phosphate buffer solution with 5×10−5m methylene green (MG) by using a two-step method. The polymer film only occurs on glassy carbon (electrodes and the reason for this is identified. The critical factor for the electropolymerization of MG lies in the preanodization on a glassy carbon) electrode, because a large amount of positive charges are accumulated and used to create the cation radicals form the polymer film. The formal potentials of PMG is pH dependent with a slope of 57mV per pH unit between pH6.0 and 8.0, which is close to the anticipated Nernstian value of 59mV for a two-electron, two-proton process. The PMG itself and the PMG on the H2O2 sensor show electrochemical behaviour with a linear plot of peak current against scan rate in the range 20 to 100mVs−1.
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N. C. Foulds and C. R. Lowe, J. Chem. Soc., Faraday Trans. l 82 (1986) 1259.
M. Umana and J. Waller, Anal. Chem. 58 (1986) 2979.
W. Schuhmann and R. Kittsteiner, Biosens. Bioelectron. 6 (1991) 263.
T. Taksuma, M. Gordaira and T. Watanabe, Anal. Chem. 64 (1992) 1183.
C. G. J. Koopal and R. J. M. Nolte, Bioelectrochem. Bioenerg. 33 (1994) 45.
N. C. Foulds and C. R. Lowe, Anal. Chem. 60 (1988) 2473.
S. Mu, J. Kan and J. Zhou, J. Electroanal. Chem. Interfa. Electrochem. 334 (1992) 121.
P. N. Barlett, P. Tebbutt and C. H. Tyrrell, Anal. Chem. 64 (1992) 138.
P. N. Barlett and P. R. Birkin, ibid. 66 (1994) 1552.
M. O. M. Berners, M. G. Boutelle and M. Fillenz, ibid. 66 (1994) 2017.
J. Wang and L. Angnes, ibid. 64 (1992) 456.
Q. Chi and S. Dong, Anal. Chim. Acta 310 (1995) 429.
A. A. Karyakin, E. E. Karyakina, W. Schumhmann, H. L. Schmidt and S. D. Varfolomeyev, Electroanalysis 6 (1994) 821.
A. A. Karyakin, A. K. Strakhova, E. E. Karyakina, S. D. Varfolomeyev and A. K. Yatsmirsky, Bioelectrochem. Bioenerg. 32 (1993) 35.
S. Dong and Q. Chu; Electroanalysis 5 (1993) 135.
T. Ohsaka, K. Tanaka and K. Toknda, J.Chem. Soc., Chem. Commun. (1993) 222.
C. Cai and K. Xue, J. Electroanal. Chem. 427 (1997) 147.
A. A. Karyakin, O. A. Bobrova and E. E. Karyakina, J. Electroanal. Chem. 399 (1995) 179.
A. Torstensson and L. Gorton, ibid. 130 (1981) 199.
F. Pariente, E. Iorenzo and H. D. Abruna, Anal. Chem. 66 (1994) 4337.
J. M. Bauldreay and M. D. Archer, Electrochim. Acta. 28 (1983) 1515.
T. Ohsaka, M. Ohba, M. Sato and N. Oyama, J. Electroanal. Chem. 300 (1991) 51.
E. C. Hurdis and H. Jr. Romeyn, Anal. Chem. 26 (1954) 320.
R. Yang, C. Ruan and J. Deng, Electrochim. Acta, in press.
D. D. Schlereth and A. A. Karyakin, J. Electroanal. Chem. 395 (1995) 221.
R. C. Engstrom, Anal. Chem. 54 (1982) 2310.
L. J. Kepley and A. J. Bard, ibid. 60 (1988) 1459.
P. M. Kovach, M. R. Deakin and R. M. Whightman, J. Phys. Chem. 90 (1986) 4612.
J. Clavilier, V. Svetlicic and V. Zutic, J. Electroanal. Chem. 386 (1995) 157.
H. Durliat, A. Courteix and M. Comptat, Bioelectrochem. Bioenerg. 22 (1989) 197.
D. J. Danner, P. J. Jr. Brignac, D. Arceneaux and V. Patel, Arch. Biochem. and Biophys. 156 (1973) 759.
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Yang, R., Ruan, C. & Deng, J. A H2O2 biosensor based on immobilization of horseradish peroxidase in electropolymerized methylene green film on GCE. Journal of Applied Electrochemistry 28, 1269–1275 (1998). https://doi.org/10.1023/A:1003460431109
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DOI: https://doi.org/10.1023/A:1003460431109