Abstract
It was found that production of superoxide (O2 – ·) is crucial for normal morphogenesis of etiolated wheat seedlings in the early stages of plant development. The development of etiolated wheat seedlings was shown to be accompanied with cyclic changes in the rate of O2 – · production both in the entire intact seedling and in its separated organs (leaf, coleoptile). First increase in the rate of O2 – · production was clearly observed in the period from two to four days of seedling development, then the rate of O2 – · production decreased to the initial level, and then it increased again for two days to a new maximum. An increase in O2 – · production in the period of the first four days of seedling development correlates with an increase in DNA and protein contents in the coleoptile. The second peak of increased rate of O2 – · production observed on the sixth or seventh day of seedling development coincides with a decrease in DNA and protein contents and apoptotic internucleosomal nuclear DNA fragmentation in the coleoptile. Incubation of seedlings in the presence of the antioxidant BHT (ionol) strongly affects their development but it does not influence the increase in DNA and protein contents for the initial four days of seedling life, and it slows down the subsequent age-dependent decrease in protein content and fully prevents the age-dependent decrease in DNA content in the coleoptile. A decrease in the O2 – · amount induced by BHT distorts the seedling development. BHT retards seedling growth, presumably by suppression of cell elongation, and it increases the life span of the coleoptile. It seems that O2 – · controls plant growth by cell elongation at the early stages of seedling development but later O2 – · controls (induces) apoptotic DNA fragmentation and protein disintegration.
Similar content being viewed by others
REFERENCES
Brar, S. S., Kennedy, T. P., Whorton, A. R., Murphy, T. M., Chitano, P., and Hoidal, J. R. (1999) J.Biol.Chem., 274, 20017-20026.
Yeh, L. H., Park, Y. J., Hansalia, R. J., Ahmed, I. S., Deshpande, S. S., Goldshmidt-Clermont, P. J., Irani, K., and Alevriadou, B. R. (1999) Am.J.Physiol., 276, 838-847.
Lee, S. L., Wang, W. W., Lanzillo, J., Gillis, C. N., and Fanburg, B. L. (1998) Biochem.Pharmacol., 56, 527-533.
Jabs, T. (1999) Biochem.Pharmacol., 57, 231-245.
Jabs, T., Dietrich, R. A., and Dangl, J. (1996) Science, 273, 1853-1856.
Levine, A., Pennell, R. I., Alvarez, M. E., Palmer, R., and Lamb, C. (1996) Curr.Biol., 6, 427-437.
Mittler, R., and Lam, E. (1996) Trends Microbiol., 4, 10-15.
Jacobson, M. D., and Raff, M. (1995) Nature, 374, 814-816.
Shimizu, S., Eguchi, Y., Kosaka, H., Kamiike, W., Matsuda, H., and Tsujimoto, Y. (1995) Nature, 374, 811-813.
Salgo, M. G., and Pryor, W. A. (1996) Arch.Biochem.Biophys., 333, 482-488.
Mittler, R., Feng, X., and Cohen, M. (1998) Plant Cell., 10, 461-473.
Kirnos, M. D., Aleksandrushkina, N. I., Shorning, B. Yu., Kudryashova, I. B., and Vanyushin, B. F. (1999) Fiziol.Rast., 46, 38-46.
Kirnos, I. D., Volkova, S. A., Ganicheva, N. I., Kudryashova, I. B., and Vanyushin, B. F. (1983) Biokhimiya, 48, 1587-1595.
May, M. J., Hammond-Kosack, K. E., and Jones, D. G. (1996) Plant Physiol., 110, 1367-1379.
Auclair, C., and Voisin, E. (1987) in Handbook of Methods for Oxygen Radical Research (Greenwald, R. A., ed.) CRC Press, Boca Raton, pp. 123-132.
Kirnos, M. D., Aleksandrushkina, N. I., and Vanyushin, B. F. (1981) Biokhimiya, 46, 1458-1474.
Bradford, M. M. (1976) Anal.Biochem., 72, 248-254.
Walker, P. R., and Sikorska, M. (1997) Biochem.Cell Biol., 75, 287-299.
Koukalova, B., Kovarik, A., Fajkus, J., and Siroky, J. (1997) FEBS Lett., 414, 289-292.
Kirnos, M. D., Aleksandrushkina, N. I., Shorning, B. Yu., Bubenshchikova, S. N., and Vanyushin, B. F. (1997) Biochemistry (Moscow), 62, 1587-1597(Russ).
Bakeeva, L. E., Kirnos, M. D., Aleksandrushkina, N. I., Kazimirchyuk, S. B., Shorning, B. Yu., Zamyatnina, V. A., Yaguzhinsky, L. S., and Vanyushin, B. F. (1999) FEBS Lett., 457, 122-125.
Lippman, R. D. (1981) J.Gerontol., 36, 550-557.
Harman, D. (1992) Mutat.Res., 275, 257-266.
Sharma, S. P., and Wadhwa, R. (1983) Mech.Ageing Dev., 23, 67-71.
Emanuel, N. M. (1976) Q.Rev.Biophys., 9, 283-308.
Ferreira, J., Coloma, L., Fones, E., Letelier, M. E., Repetto, Y., Morello, A., and Aldunate, J. (1988) FEBS Lett., 234, 485-488.
Bolton, J. L., and Thompson, J. A. (1991) Drug.Metab.Dispos., 19, 467-472.
Shorning, B. Yu., Poleshchyuk, S. V., Gorbatenko, I. Yu., and Vanyushin, B. F. (1999) Izv.RAN.Ser.Biol., 26, 30-38.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shorning, B.Y., Smirnova, E.G., Yaguzhinsky, L.S. et al. Necessity of Superoxide Production for Development of Etiolated Wheat Seedlings. Biochemistry (Moscow) 65, 1357–1361 (2000). https://doi.org/10.1023/A:1002892520658
Issue Date:
DOI: https://doi.org/10.1023/A:1002892520658