Skip to main content
Log in

A comparative study on the frequency of prophages among natural isolates of Salmonella and Escherichia coli with emphasis on generalized transducers

  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Several collections of natural isolates of the genus Salmonella and of the species Escherichia coli were studied for the release of viable temperate phages. The results indicated that functional prophage genomes may be a common constituent of all bacterial genomes of the investigated strains. About 99% of the Salmonella phages are capable of generalized transduction of chromosomal host markers and plasmids. The ratio of transducing E. coli phages is significantly lower.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beltran P, Plock SA, Smith NH, Whittam TS, Old DC & Selander RK (1991) Reference collection of strains of the Salmonella typhimuriumcomplex from natural populations. J. Gen. Microbiol. 137: 601-606

    Google Scholar 

  • Bertani G & Weigle JJ (1953) Host controlled variation in bacterial viruses. J. Bacteriol. 65: 113-121

    Google Scholar 

  • Boyd EF, Wang FS, Beltran P, Plock SA, Nelson K & Selander RK (1993) Salmonellareference collection B (SARB): strains of 37 serovars of subspecies I. J. Gen. Microbiol. 139: 1125-1132

    Google Scholar 

  • Bullas LR & Ryu JI (1983) Salmonella typhimuriumLT2 strains which are r-m+ for all three chromosomally located systems of DNA restriction and modification. J. Bacteriol. 156: 471-474

    Google Scholar 

  • Jackson EN, Jackson DA & Deans RJ (1978) EcoRI analysis of bacteriophage P22 DNA packaging. J. Mol. Biol. 118: 365-388

    Google Scholar 

  • Kinkeldey U, von Lieres D & Schmieger H (1978) Bacterial donor mutants affecting the efficiency of generalized transduction by Salmonellaphage P22. J. Gen. Microbiol. 108: 227-237

    Google Scholar 

  • Mills DM, Bajaj V & Lee CA (1995) A 40 kb chromosomal fragment encoding Salmonella typhimuriuminvasion genes is absent from the corresponding region of the Escherichia coliK-12 chromosome. Mol. Microbiol. 15: 749-759

    Google Scholar 

  • Ochman H & Selander RK (1984) Standard reference strains of Escherichia colifrom natural populations. J. Bacteriol. 157: 690-693

    Google Scholar 

  • Sambrook J, Fritsch EF & Maniatis T (1989) Molecular cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Schicklmaier P & Schmieger H (1995) Frequency of generalized transducing phages in natural isolates of the Salmonella typhimuriumcomplex. Appl. Environ. Microbiol. 61: 1637-1640

    Google Scholar 

  • Shea JE, Hensel M, Gleeson C & Holden DW (1996) Identification of a virulence locus encoding a second type III secretion system in Salmonella typhimurium. Proc. Natl. Acad. Sci. U.S.A. 93: 2593-2597.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schicklmaier, P., Moser, E., Wieland, T. et al. A comparative study on the frequency of prophages among natural isolates of Salmonella and Escherichia coli with emphasis on generalized transducers. Antonie Van Leeuwenhoek 73, 49–54 (1998). https://doi.org/10.1023/A:1000748505550

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1000748505550

Navigation