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Morphological and molecular analyses of larval taeniid species in small mammals from contrasting habitats in Denmark

Published online by Cambridge University Press:  28 October 2013

M.N.S. Al-Sabi ,
P.M. Jensen ,
M.U. Christensen  and
C.M.O. Kapel
M.N.S. Al-Sabi*
Affiliation:
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871Frederiksberg C, Denmark National Veterinary Institute, Technical University of Denmark, DK-1870Frederiksberg C, Denmark
P.M. Jensen
Affiliation:
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871Frederiksberg C, Denmark
M.U. Christensen
Affiliation:
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871Frederiksberg C, Denmark
C.M.O. Kapel
Affiliation:
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871Frederiksberg C, Denmark
*
*E-mail: moals@vet.dtu.dk
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Abstract

Taeniid infections in intermediate hosts manifest themselves as extraintestinal larval stages which, in early development, lack species-specific characteristics. The inability to distinguish infections of zoonotic importance such as Echinococcus multilocularis from other taeniid infections that have mainly veterinary significance stimulated the development of species-specific molecular diagnostics. In this study, the prevalence of taeniid infections in potential intermediate hosts was evaluated using both morphological diagnosis and a newly described multiplex polymerase chain reaction (PCR) for species determination. Small mammals (N= 719) were trapped in three different types of habitats in north-east Zealand, Denmark. The sensitivity of the multiplex PCR (90.5%) exceeded that of morphological examination (57.9%) for identifying 95 taeniid infections. The use of the multiplex PCR resulted in higher prevalence rates due to improved detection of immature liver infections with Hydatigera taeniaeformis and Versteria mustelae, but did not affect the observed prevalence rates of peritoneal metacestodes of Taenia polyacantha. The prevalence of taeniid infections showed a significant difference according to habitat type, potentially identifying a ‘sylvatic’ transmission and an ‘urban’ transmission, with marked variation among different taeniid species. Versteria mustelae and T. polyacantha were more prevalent in rural forests, while infections with H. taeniaeformis were dominant in urban parks/forests and in residential and farm gardens. The multiplex PCR facilitated a better utilization of wildlife samples by yielding a higher number of definitive diagnoses of ambiguous taeniid infections in liver lesions, allowing for more accurate epidemiological data and, hence, a more accurate risk assessment.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 1 , January 2015 , pp. 112 - 117
Copyright
Copyright © Cambridge University Press 2013 

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